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  4. Recombinant Mouse Coronin-1A (Coro1a)

Recombinant Mouse Coronin-1A (Coro1a)

  • 货号：
    CSB-YP005813MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005813MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005813MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005813MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005813MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Coro1a
  • Uniprot No.：
  • 别名：
    Coro1a; Coro1; Coronin-1A; Coronin-like protein A; Clipin-A; Coronin-like protein p57; Tryptophan aspartate-containing coat protein; TACO
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-461
  • 氨基酸序列
    SRQVVRSSK FRHVFGQPAK ADQCYEDVRV SQTTWDSGFC AVNPKFMALI CEASGGGAFL VLPLGKTGRV DKNVPLVCGH TAPVLDIAWC PHNDNVIASG SEDCTVMVWE IPDGGLVLPL REPVITLEGH TKRVGIVAWH PTAQNVLLSA GCDNVILVWD VGTGAAVLTL GPDVHPDTIY SVDWSRDGAL ICTSCRDKRV RVIEPRKGTV VAEKDRPHEG TRPVHAVFVS EGKILTTGFS RMSERQVALW DTKHLEEPLS LQELDTSSGV LLPFFDPDTN IVYLCGKGDS SIRYFEITSE APFLHYLSMF SSKESQRGMG YMPKRGLEVN KCEIARFYKL HERKCEPIAM TVPRKSDLFQ EDLYPPTAGP DPALTAEEWL GGRDAGPLLI SLKDGYVPPK SRELRVNRGL DSARRRATPE PSGTPSSDTV SRLEEDVRNL NAIVQKLQER LDRLEETVQA K
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.
  • 基因功能参考文献：
    1. our findings indicate that coronin 1A is a pivotal component that regulates lysosomal fusion and the secretion pathway in osteoclast-lineage cells and may provide a novel therapeutic target for bone diseases. PMID: 28300073
    2. these data suggest that coronin 1 is dispensable for mouse NK cell homeostasis and function. PMID: 27717523
    3. Results suggest the existence of a hitherto unrecognized coronin 1-dependent decision switch early during life that is responsible for peripheral naive T cell survival and homeostasis. PMID: 28821585
    4. Coro1A represents an important novel player in integrin biology, with key functions in polymorphonuclear neutrophils trafficking during innate immunity. PMID: 28615221
    5. the phenotype of T cell-specific Coronin 1a deletion resembles the phenotype observed with conventional (whole body) Coronin 1a knock-out mice. In summary, our findings provide formal proof of the predominant T cell-intrinsic role of Coronin 1A. PMID: 27566541
    6. s report that this mutation induces pleiotropic effects in Coro1A protein, including the exacerbation of Coro1A-dependent actin-binding and -bundling activities. PMID: 26108624
    7. Together, these findings both in Jurkat T cells as well as in primary T cells indicate a regulatory role of Coro1A on PKCtheta; recruitment and function downstream of the TCR leading to NF-kappaB transactivation. PMID: 25889880
    8. An essential role for 14-3-3 zeta in the regulation of macropinocytosis in macrophages upon cytokine stimulation through modulation of the localization of coronin 1. PMID: 25645340
    9. this work suggests that periods before and after NGF-TrkA-induced Coronin-1 expression (and likely other factors) defines two distinct axon growth states PMID: 25740518
    10. Absence of coronin 1A is associated with severe combined immunodeficiency in humans, hypomorphic mutations lead to a profound defect in naive T cells, expansion of oligoclonal memory T cells, and susceptibility to Epstein Barr B lymphoproliferation. PMID: 25269405
    11. Our results indicate that Abp1 negatively regulates BCR signaling by coupling actin remodeling to B-cell contraction and activation of inhibitory signaling molecules PMID: 24958882
    12. Altered T-cell entry and egress in the absence of Coronin 1A attenuates murine acute graft versus host disease. PMID: 24752751
    13. These results suggest that coronin 1, while important for naive T cell survival, is dispensable for other leukocyte function under inflammatory conditions in vivo. PMID: 23856257
    14. The results of this study establish Coronin-1 as an essential component of a feedback loop that mediates NGF-TrkA endosome stability, recycling and signaling as a critical mechanism governing developmental competition for survival. PMID: 24270184
    15. Data suggest that coronin 1 plays a differential role in CD8(+) versus CD4(+) T cell responses and activation. PMID: 23933558
    16. Coronin 1 is dispensable for antigen processing and presentation by dendritic cells. PMID: 23099476
    17. These results suggest that Coro1a inhibits autophagosome formation in alveolar macrophages, thereby facilitating Mycobacterium tuberculosis survival within the lung. PMID: 22256790
    18. Coro1A controls actin association with endocytic organelles, thereby negatively regulating endo-lysosomal delivery, degradation of modified lipoproteins and cholesterol deposition in macrophages. PMID: 22223354
    19. GRAIL functions via the ubiquitination and degradation of actin cytoskeleton-associated proteins, in particular Arp2/3-5 and coronin 1A PMID: 22016387
    20. required for quiescence and maintenance of adult hematopoietic stem cells PMID: 21885021
    21. Differential regulation of mast cell degranulation versus cytokine secretion by the actin regulatory proteins Coronin1a and Coronin1b. PMID: 21844203
    22. Coronin 1 is crucial for maintenance of the naive T-cell pool and for development of murine experimental autoimmune encephalomyelitis. PMID: 21289301
    23. Coronin-1A activity is not required for mast cell function. PMID: 20643816
    24. expressed 10-23 DNAzyme targeting taco gene can reduce TACO expression both at mRNA level (by 78.26%) and at protein level (by 75.30%). PMID: 20059315
    25. The leukocyte plasma membrane associates with the actin cytoskeleton through coronin-1. PMID: 15800061
    26. coronin-1 is required for an early step in phagosome formation, consistent with a role in actin polymerization PMID: 15829569
    27. Analysis of charged and conserved surface residues of coronin-1 delineate possible binding sites for F-actin on the beta propeller PMID: 16407068
    28. results show coronin 1 exerted an inhibitory effect on cellular steady-state F-actin formation by an Arp2/3-dependent mechanism in T lymphocytes; it was required for chemokine-mediated migration but dispensable for T cell antigen receptor functions PMID: 16902139
    29. These results demonstrate a role for coronin 1 in activating Ca(2+) dependent signaling processes in macrophages and reveal a function for calcineurin in the regulation of phagosome-lysosome fusion upon mycobacterial infection. PMID: 17632055
    30. These findings provide further support for the relevance of the LpdC-coronin-1 interaction in phagosome maturation arrest. PMID: 17652161
    31. Therefore, these results show that in J774 cells coronin 1 has a specific role in modulating phagosome-lysosome transport upon mycobacterial infection and that it is dispensable for most F-actin-mediated cytoskeletal rearrangements. PMID: 18162581
    32. a lupus-suppressing locus is caused by a nonsense mutation of the filamentous actin-inhibiting Coronin-1A gene PMID: 18199416
    33. Coronin-1, through activation of Ca2+ release from intracellular stores, is an essential regulator of peripheral lymphocyte survival. PMID: 18345003
    34. Findings establish a function for coronin 1A in T cell egress, identify a surface of coronin involved in Arp2/3 regulation. PMID: 18836449
    35. Coronin-1A links cytoskeleton plasticity with the functioning of discrete TCR signaling components PMID: 18941544
    36. Coronin 1 is dispensable for F-actin-dependent processes and oxidase activity in neutrophils; instead, it is involved in the regulation of calcium dependent signaling reactions. PMID: 19234169

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Cytoplasmic vesicle, phagosome membrane. Note=In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification.
  • 蛋白家族：
    WD repeat coronin family
  • 组织特异性：
    Expressed in spleen, lymph nodes, thymus, brain and at very lower levels in lung. Also expressed in cells of the lymphoid/myeloid lineage. Not expressed in Kuffper cells.
  • 数据库链接：

    KEGG: mmu:12721

    STRING: 10090.ENSMUSP00000032949

    UniGene: Mm.290482