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  4. Recombinant Mouse Cyclin-dependent kinase 6 (Cdk6)

Recombinant Mouse Cyclin-dependent kinase 6 (Cdk6)

  • 货号：
    CSB-YP005074MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005074MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005074MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005074MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005074MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cdk6; Cdkn6; Crk2Cyclin-dependent kinase 6; EC 2.7.11.22; CR2 protein kinase; CRK2; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-326
  • 氨基酸序列
    MEKDSLSRAD QQYECVAEIG EGAYGKVFKA RDLKNGGRFV ALKRVRVQTS EEGMPLSTIR EVAVLRHLET FEHPNVVRLF DVCTVSRTDR ETKLTLVFEH VDQDLTTYLD KVPEPGVPTE TIKDMMFQLL RGLDFLHSHR VVHRDLKPQN ILVTSSGQIK LADFGLARIY SFQMALTSVV VTLWYRAPEV LLQSSYATPV DLWSVGCIFA EMFRRKPLFR GSSDVDQLGK ILDIIGLPGE EDWPRDVALP RQAFHSKSAQ PIEKFVTDID ELGKDLLLKC LTFNPAKRIS AYGALNHPYF QDLERYKDNL NSHLPSNQST SELNTA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases.
  • 基因功能参考文献：
    1. The downregulation of Cdkn1a and the upregulation of Cdk6. PMID: 29357734
    2. data indicate that Gli3 controls the onset of cortical neurogenesis by determining the levels of Cdk6 expression, thereby regulating neuronal output and cortical size. PMID: 30093555
    3. Cdk6 interacts with a number of proteins involved in cytoskeleton organization. Cdk6 regulates the transcription of a panel of genes involved in actin (de-)polymerization. PMID: 28255017
    4. CDK6-mediated suppression of CD25 is required for initiation of T-ALL by activated Notch1. . Pharmacologic inhibition of CDK6 kinase induces CD25 and RUNX1 expression, cell cycle arrest and apoptosis in mouse and human T-ALL. PMID: 26707936
    5. Fbxo7-deficient immature thymocytes failed to undergo expansion in the thymus due to a lack of Cdk6 activity, while mature T cells showed enhanced proliferative capacity upon T-cell receptor engagement due to reduced p27 levels. These studies reveal differential cell cycle regulation by Fbxo7 at different stages in T-cell development. PMID: 27915416
    6. Taken together, our results suggested that microRNA-33 enhanced the replicative senescence of MEFs potentially by suppressing CDK6 expression. PMID: 27059142
    7. Data show that E11/glycoprotein 38(GP38) was up-regulated upon SEMA3A stimulation, and cyclin-dependent kinase 6 (CDK6) was down-regulated in a time-dependent manner. PMID: 27107080
    8. Notch3 transcription and growth of lymphoma cells was dependent on CDK6. PMID: 25483078
    9. CDK6 is an important regulator of stem cell activation and an essential component of a transcriptional complex that suppresses Egr1 in hematopoietic and leukemic stem cell activation PMID: 25342715
    10. Cdk6 deficiency prevents the expansion of neuronally committed precursors by lengthening G(1) phase duration, reducing concomitantly the production of newborn neurons PMID: 21319271
    11. a mechanism for LPS signaling which involves a decrease in miR-107 leading to an increase in CDK6 PMID: 21628465
    12. an important role for CDK6 kinase activity in thymocyte development that operates partially through modulating Notch target gene expression. PMID: 21508411
    13. we identify CDK6 as relevant and critical target of AP-1-regulated DNA methylation on BCR-ABL-induced transformation, thereby accelerating leukemogenesis PMID: 21300982
    14. These studies provide in vivo evidence that CDK4 and CDK6 play a similar role as a mediator of keratinocyte proliferation but differ in apoptosis activation and skin tumor development. PMID: 21224071
    15. This study propose that chronic inflammation decreases spinal microglial/macrophage GRK2, which prevents silencing of microglia/macrophage activity and thereby contributes to prolonged hyperalgesia. PMID: 20609517
    16. Data show that IO treatment provoked an increase in CDK6, Cyclin E, p27kip1, and p21waf1 expression, and after 6BIO treatment, the expression of most of these proteins decreased. PMID: 19860623
    17. PU.1 coordinates proliferation and differentiation in immature erythroid cells by inhibiting the GATA-1-mediated gene expression program and also by regulating expression of cdk6 and genes that control progression through the G(1) phase of the cell cycle PMID: 19955566
    18. Evidence for coordinated interaction of cyclin D3 with p21 and cdk6 in directing the development of uterine stromal cell decidualization and polyploidy during implantation PMID: 11804782
    19. p18(INK4C) inhibits CD40-signaled cell cycle progression by preferential inhibition CDK6 PMID: 12196289
    20. adapter Crk-II is constitutively associated with C3G, a GDP to GTP exchange factor for several small GTP-binding proteins PMID: 12215429
    21. CDK6 blocks differentiation, coupling cell proliferation to the block to differentiation in leukemic cells PMID: 12833137
    22. expression of cdk6 resulted in a distinct and rapid morphology change from multipolar to bipolar PMID: 12861051
    23. oocytes regulators amounts and localization undergo dramatic changes as the oocytes grow, meiotically mature, and transit into embryogenesis and CDK4, CDK6, cyclin D2, and p27 appear in unusual, most likely posttranslationally modified, forms. PMID: 13679319
    24. Cdk6 is a critical regulator of BMP-2-induced osteoblast differentiation and its Smads-mediated down-regulation is essential for efficient osteoblast differentiation PMID: 15254224
    25. Cdk6 may be a regulator of chondrocyte differentiation and that its p38-mediated downregulation is involved in the efficient differentiation PMID: 15795936
    26. cdk6 expression in primary astrocytes favored the expression of progenitor cell markers PMID: 16767702
    27. Results show a critical requirement for CDK6 in Notch/Akt-dependent T-cell development and tumorigenesis. PMID: 19155308
    28. These findings identified Znhit1 as a CDK6 regulator that plays an important role in cell proliferation. PMID: 19501046
    29. A majority of cdk6 is localized to the cytoplasm with concentrations of cdk6 in the edges of the cytoplasm and in the cytoplasmic extensions of cells. PMID: 19667758

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell projection, ruffle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
  • 组织特异性：
    Expressed in subgranular zone (SGZ) of the hippocampal dentate gyrus (DG) and the subventricular zone (SVZ) of the lateral ventricles whose neural precursor cells (NPC) give rise to dentate granule neurons and olfactory bulb (OB) interneurons, respectivel
  • 数据库链接：

    KEGG: mmu:12571

    STRING: 10090.ENSMUSP00000037925

    UniGene: Mm.31672