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  4. Recombinant Mouse Cyclin-dependent kinase inhibitor 1B (Cdkn1b)

Recombinant Mouse Cyclin-dependent kinase inhibitor 1B (Cdkn1b)

  • 货号：
    CSB-YP005087MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005087MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005087MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005087MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005087MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cdkn1b; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-197
  • 氨基酸序列
    MSNVRVSNGS PSLERMDARQ AEHPKPSACR NLFGPVNHEE LTRDLEKHCR DMEEASQRKW NFDFQNHKPL EGRYEWQEVE RGSLPEFYYR PPRPPKSACK VLAQESQDVS GSRQAVPLIG SQANSEDRHL VDQMPDSSDN PAGLAEQCPG MRKRPAAEDS SSQNKRANRT EENVSDGSPN AGTVEQTPKK PGLRRQT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
  • 基因功能参考文献：
    1. after irradiation, loss of p27 is associated to accumulation of residual DNA damage and increased number of mitotic aberration. We show that p27 expression is necessary to preserve genomic integrity and for correct recognition and clearing-out of aberrant cells. PMID: 28377607
    2. Although expressed in all retinal cell types, p27 is required to maintain the quiescence of specific cell types including bipolar cells, Muller glia, and cones while it is dispensable for preventing cell cycle reentry in other cell types PMID: 28931408
    3. Findings demonstrate functional interplay between a transcriptional regulator and p27(kip1) to mediate neuroprogenitor cell cycle exit, as well as to promote radial migration through a molecular mechanism consistent with suppression of RhoA signaling. PMID: 28506232
    4. Data show that both bone marrow-derived and gastric epithelial cells contribute to the increased gastric cancer susceptibility of p27-deficient Helicobacter pylori (H. pylori) -infected mice. PMID: 27655701
    5. Our results revealed that CKS1 is involved in normal glutamatergic synapse development and dendritic spine maturation in adult hippocampus through modulating p27 stability. PMID: 28365778
    6. p27kip1 is involved in the decisive step around cell-cycle exit and plays an important role in activity-regulated and functionally relevant adult hippocampal neurogenesis. PMID: 27790794
    7. p27(Kip1)-target genes are involved in cell adhesion and cell signaling in mouse embryonic fibroblasts. PMID: 29155860
    8. This study demonstrates that LMP2A uses the role of MYC in the cell cycle, particularly in the p27(kip1) degradation process, to accelerate lymphomagenesis in vivo. PMID: 29074502
    9. Our findings demonstrate a direct role for microRNAs in controlling the rate of liver regeneration after injury. PMID: 28232457
    10. systems-level control of cell cycle arrest by pRB-E2F and p27-CDK regulation, is reported. PMID: 27821477
    11. Results demonstrated that the addition of oncogenic mutations, such as loss of p27 or p53, promotes ovarian tumor development from the benign adenomas of germ cell-deficient ovaries. PMID: 27354067
    12. p27 inactivation promotes injury islet graft loss via the elevation of proliferation and inflammatory cytokines secretion in infiltrating macrophages which induced nonspecific inflammation independent of TNF-alpha/nuclear factor-kappa b pathway. T PMID: 27631551
    13. Knockout of p27 enhances arterial collateralization in response to hindlimb ischemia through enlargement of a new collateral pathway. PMID: 25701497
    14. Cdkn1b exert its effects via the inhibition of proliferation and is mediated by miR-24 and targeted by the transcription factors FOXO4 and AP4 in the peroxidasin (Pxdn) mutation-induced eye disorders, such as glaucoma. PMID: 27121343
    15. These results suggest that fad24 may have an important role in the S phase re-entry of quiescent C2C12 cells through the regulation of p27(Kip1) at the protein level PMID: 26902224
    16. The down-regulation of p27 and the activation of mTOR pathway may be involved in miR-222-induced heart failure and autophagy inhibition. PMID: 27614440
    17. Used LDLr knockout mice to identify the effects of Kiom-18 as a preliminary result in an atherosclerosis animal model; the level of p27(kip1) expression was upregulated in the presence of the Kiom-18 extract. PMID: 27465365
    18. Nanog enhances proliferation of fibroblasts through transcriptional regulation of cell cycle inhibitor p27 gene. PMID: 26795560
    19. Mice depleted for p27Kip1 in a Cdk4 R24C background develop pituitary tumors with complete penetrance and very short latencies. PMID: 26292757
    20. autophagy is required for maintaining the expression level of CDKN1B in naive T cells and selectively degrades CDKN1B after TCR stimulation PMID: 26569626
    21. nuclear localization sequence and C-terminus of PTHrP play a critical role in preventing skin from premature ageing that is partially mediated by p27 PMID: 26121068
    22. The overexpression of p27 attenuates tumorigenicity by reducing proliferation and earlier loss of stemness of mouse induced pluripotent stem cells. PMID: 26880084
    23. p27(Kip1) and p21(Cip1) are insufficient for the proliferative inhibition of smooth muscle cells cultured in type 1 collagen. honeycombs. PMID: 26522285
    24. results implicate Eya4/Six1 regulates normal cardiac function via p27/casein kinase-2alpha/histone deacetylase 2 and indicate that mutations within this transcriptional complex and signaling cascade lead to the development of cardiomyopathy. PMID: 26499333
    25. the p27 pathway may function downstream in the action of PTHrP to regulate skeletal growth and development. PMID: 25917430
    26. Findings indicate that the Cdkn1b gene is positively regulated by a cis element at the promoter of the adjacent Lockd locus, whereas the transcribed Lockd lncRNA is dispensable for this function. PMID: 27041223
    27. These results suggest that the abnormal expression of miR222 may contribute to dysregulation of the cellcycle in Alzheimer's disease, at least in part by affecting the expression of p27Kip1. PMID: 26398571
    28. Data demonstrate that the deletion of cyclin-dependent kinase inhibitor p27 (p27) in parathyroid hormone like hormone (Pthrp) knock-in(KI/KI) mice can partially rescue defects in dental and mandibular development. PMID: 26859332
    29. Data indicate that an AMP-ctivated kinase (AMPK)/cell-cycle inhibitor p27KIP1 (p27) axis drives activation of autophagy in rapamycin-treated tuberous sclerosis 2 protein (Tsc2)-null cells. PMID: 26975583
    30. Study shows that SGK1 impairs nuclear abundance of p27 and suggests that phosphorylation of p27T197 by SGK1 is required for cardiac hypertrophy. PMID: 26344141
    31. MARCKS knockdown arrested VSMC cell cycle by decreasing KIS expression. Decreased KIS expression resulted in nuclear trapping of p27kip1 in VSMCs. PMID: 26528715
    32. Report defective erythropoiesis in transgenic mouse with reduced Fbxo7 expression due to decreased erythrocyte p27 expression. PMID: 26095538
    33. Data (including data from studies in knockout/transgenic mice) suggest CDKN1B-RB1-E2F1 (CDKN1B-retinoblastoma 1-E2F transcription factor 1) pathway is essential for SSC (spermatogonial stem cell) self-renewal and protection against genomic damage. PMID: 25959802
    34. Sphingomyelin synthase 1 regulates Neuro-2a cell proliferation and cell cycle progression through modulation of p27 expression and Akt signaling PMID: 25084761
    35. identifies a regulatory axis controlling the G1/S-phase transition, relying on the regulation of MT stability by p27(kip1) and finely controlling the spatiotemporal activation of the Ras-MAPK signaling pathway PMID: 26512117
    36. p27(kip1) acts as a central player of the molecular program that keeps adult hippocampal stem cells out of the cell cycle. PMID: 25185890
    37. The p27(kip1) cooperates with stathmin to control the early phase of G1 to S phase transition and that this function may be of particular relevance in the context of tumor progression. PMID: 25486569
    38. By targeting Ras/ERK pathway, p27 provided a negative control over HIF-1alpha protein synthesis in an S6-dependent manner, and abrogated arsenite-induced cell transformation via downregulation of HIF-1alpha translation. PMID: 25412313
    39. P27 plays a negative regulatory role in dentin formation and alveolar bone development. PMID: 24916068
    40. p27(kip1) promotes mesenchymal migration and hinders amoeboid migration upstream of the Rho/ROCK pathway. PMID: 25015295
    41. Data show that phosphorylation of p27Kip1 threonine 187 (p27T187) is important in retinoblastoma protein Rb1-deficient tumorigenesis and immunity in aging. PMID: 25583987
    42. Kip1 has an important role in HDAC inhibitors -mediated cell cycle arrest at G1/S but not HDAC inhibitors -induced apoptosis. PMID: 24292681
    43. Data indicate that the positive rates of cyclin-dependent kinase inhibitor p27 (p27) immunocytochemical reaction were higher in the octreotide (OCT)-treated tumors. PMID: 24694834
    44. These results show that p27(Kip1) actively maintains hair cells quiescence in postnatal mice PMID: 25411503
    45. This study suggests that PTEN regulates p27kip1 through p-Akt, thereby regulating the proliferation and differentiation of auditory progenitors PMID: 24481416
    46. involved in the chronic AMP-activated protein kinase activation that inhibits mesenchymal stem cell proliferation PMID: 24104879
    47. Propose a receptor-specific function for Notch2 in regulating Jag-1-induced p27(kip1) expression and growth arrest in vascular smooth muscle cells. PMID: 23965337
    48. p27 represses the proliferation of LCs, which is specifically required for maintaining the quiescent and drug-resistant states of a small subset of MA9-LSCs in collaboration with the differentiation blockage function of c-Myc. PMID: 23988911
    49. Restriction of self-renewal potential to label-retaining cells is established early in life and is maintained during increased tissue turnover through the cell cycle inhibitor p27(kip1). PMID: 24715455
    50. Cdkn1b overexpression in adult mice alters the balance between genome and tissue aging. PMID: 24149709

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Endosome.
  • 蛋白家族：
    CDI family
  • 数据库链接：

    KEGG: mmu:12576

    STRING: 10090.ENSMUSP00000003115

    UniGene: Mm.2958