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  4. Recombinant Mouse Cystathionine beta-synthase (Cbs)

Recombinant Mouse Cystathionine beta-synthase (Cbs)

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  • 中文名称：
    Recombinant Mouse Cystathionine beta-synthase(Cbs)
  • 货号：
    CSB-EP849665MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Cbs
  • Uniprot No.：
  • 别名：
    Cbs; Cystathionine beta-synthase; EC 4.2.1.22; Beta-thionase; Serine sulfhydrase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    65.4kDa
  • 表达区域：
    2-561aa
  • 氨基酸序列
    PSGTSQCEDGSAGGFQHLDMHSEKRQLEKGPSGDKDRVWIRPDTPSRCTWQLGRAMADSPHYHTVLTKSPKILPDILRKIGNTPMVRINKISKNAGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERAGNLKPGDTIIEPTSGNTGIGLALAAAVKGYRCIIVMPEKMSMEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLKNEIPNSHILDQYRNASNPLAHYDDTAEEILQQCDGKLDMLVASAGTGGTITGIARKLKEKCPGCKIIGVDPEGSILAEPEELNQTEQTAYEVEGIGYDFIPTVLDRAVVDKWFKSNDEDSFAFARMLIAQEGLLCGGSSGSAMAVAVKAARELQEGQRCVVILPDSVRNYMSKFLSDKWMLQKGFMKEELSVKRPWWWRLRVQELSLSAPLTVLPTVTCEDTIAILREKGFDQAPVVNESGAILGMVTLGNMLSSLLAGKVRPSDEVCKVLYKQFKPIHLTDTLGTLSHILEMDHFALVVHEQIQSRDQAWSGVVGGPTDCSNGMSSKQQMVFGVVTAIDLLNFVAAREQTQT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.
  • 基因功能参考文献：
    1. Data suggest that impaired cystathionine beta-synthase-hydrogen sulfide axis may contribute to the pathogenesis of Parkinson's disease, and that modulation of this axis may become a novel therapeutic approach for Parkinson's disease. PMID: 28774789
    2. results suggested that reduced cystathionine beta-synthase/hydrogen sulfide AMP-activated protein kinase (CBS-H2S-AMPK) cascade activity contributed to microglia-mediated neuroinflammation following stroke. PMID: 28751019
    3. Results indicate that activation of the cystathionine-beta-synthase (CBS) and its product hydrogen sulfide (CBS/H2S axis promotes colon carcinogenesis. PMID: 28923859
    4. showed that N-homocysteinylation of collagen in Cbs(-/-) mice impairs its cross-linking PMID: 27530978
    5. the findings of this study indicate that a deficiency in 3MST does not significantly affect endotoxemia, while a deficiency in CBS or CSE slightly ameliorates the outcome of LPS-induced endotoxemia in vivo. PMID: 27748832
    6. These data confirm a key role for the H2S-generating enzymes Cbs and Cth in pulmonary vascular development and homeostasis and in lung alveolarization. PMID: 26232299
    7. genetic deficiency results in reduced Tet1 and Tet2 expression, which leads to hypermethylation of Foxp3, and impairment of Treg cell differentiation and immune homeostasis. PMID: 26275994
    8. Elevated homocytsteine levels in the CBS-/+ mouse skeletal muscles caused diminished anti-oxidant capacity and contributed to enhanced total protein as well as PGC-1alpha specific nitrotyrosylation after ischemia. PMID: 25608649
    9. CBS disruption causes embryo retention and developmental delay in the mouse oviduct. PMID: 24914509
    10. CBS is present in adrenocortical cells and accounts for H2S generation in adrenal glands. CBS is critical for maintenance of mitochondrial function and glucocorticoid production in adrenal cortex. PMID: 24702258
    11. Elevating endogenous H2S via CBS over-expression in immortalized microglia not only reduced the expression of pro-inflammatory M1 genes, but also enhanced the anti-inflammatory M2 marker IL-10 production. PMID: 25086357
    12. Cbs protein expression is upregulated in brain cortex following traumatic brain injury. PMID: 23315129
    13. Brain of mice heterozygous for Cbs displays overexpression of DYR kinase and SAH hydrolase. PMID: 22700376
    14. These findings set the stage to investigate the role of CBS and the transsulfuration pathway in the generation of GSH in mouse retina. PMID: 23470016
    15. Hydrogen sulfide in the cortex and hippocampus exhibited dynamic changes after brain injury, in parallel with CBS mRNA and protein expression PMID: 23325453
    16. Lanthionine synthetase C-like protein 1 interacts with and inhibits cystathionine beta-synthase: a target for neuronal antioxidant defense. PMID: 22891245
    17. Cystathionine beta-synthase deficiency causes infertility by impairing decidualization and gene expression networks in uterus implantation sites. PMID: 22617046
    18. Severe hyperhomocysteinemia promotes bone marrow-derived and resident inflammatory monocyte differentiation and atherosclerosis in LDLr/CBS-deficient mice. PMID: 22628578
    19. A survey of hemostatic and hemodynamic parameters revealed no detectible differences between control and Tg-I278T Cbs(-/-) mice. PMID: 22186991
    20. Results show that lack of CBS activity causes loss of fat mass, and that this effect appears to be independent of low serum tCys PMID: 22096601
    21. cbs is a primary 1,25-dihydroxyvitamin D(3)[1,25(OH)(2) D(3)- target gene which renders homocyeteine metabolism responsive to 1,25(OH)(2) D(3). PMID: 21898591
    22. Hyperhomocysteinemia impaired nitric oxide and endothelium-derived hyperpolarizing factor (EDHF) - mediated, endothelium-dependent relaxations of small mesenteric arteries in transgenic cystathionine beta synthase-deficient mice.[EDHF] PMID: 21653942
    23. cystathionine beta-synthase activity partially regulates endogenous H(2)S PMID: 20955694
    24. CBS expression is under dietary control; CBS is down-regulated within a few days upon initiation of methionine-deficient diet; such regulation is post-transcriptional in nature. PMID: 20036517
    25. The effects of CBS AND CSE gene transfer on the homocysteine-mediated inflmmation of mesangial cells induced by hydrogen sulfide and MCP-1 and MIP-2 induction are reported. PMID: 20943958
    26. We report here the generation of a new mouse model of classical homocystinuria in which the mouse cbs gene is inactivated and that exhibits low-level expression of the human CBS transgene under the control of the human CBS promoter PMID: 20638879
    27. birth incidence of cbs (-/-) mice produced from heterozygous crosses is non-Mendelian and not significantly improved by treatment with either the Hcy lowering compound betaine or the cysteine donor N-acetylcysteine. PMID: 20638882
    28. Cysteinemia, rather than homocysteinemia, is associated with plasma apolipoprotein A-I levels in hyperhomocysteinemia: lipid metabolism in cystathionine beta-synthase deficiency. PMID: 20537649
    29. cystathionine beta-synthase has a role in hepatic steatosis through abnormal lipid metabolism PMID: 15466479
    30. active CBS is essential for the formation of cysteinylated plasma albumin (CysAlb) PMID: 15555590
    31. hyperhomocysteinemia in liver of CBS-deficient mice promotes oxidative stress, which may cause mitochondrial damage in association with activation of hepatic stellate cells, leading to liver injury PMID: 15887121
    32. cystathionine beta-synthase plays a crucial role in the development and maintenance of the central nervous system PMID: 16160063
    33. Caerulein decreased the level of cystathionine beta-Synthase. PMID: 17488480
    34. Role of myofibroblasts differentiation in case of lung fibrosis due to CBS deficiency in mice. PMID: 17543941
    35. Cystathionine beta synthase secreted by granulosa cells is necessary for oocyte maturation. PMID: 17561372
    36. There was good CBS activity in the liver and cerebellum of the mouse but no activity in the retina. PMID: 17853447
    37. Transfection of endothelial cells with CBS gene construct reduced homocysteine accumulation in high methionine-fed cells. PMID: 17855772
    38. Cystathionine beta-synthase played essential roles in the central nervous system function and cysteine biosynthesis. PMID: 18364386
    39. Double heterozygous mice lacking one allele of Cbs and Apoa1 develop hyperhomocysteinemia and hypoalphalipoproteinemia together with moderate hypertension. PMID: 18508577
    40. cystathionine beta synthase deficiency initiates redox disequilibrium in the liver PMID: 18541157
    41. The effects of different degrees of Cbs deficiency on hyperhomocysteinemia symptoms are reported. PMID: 18987302
    42. Left ventricular hypertrophy was exaggerated in double knockout, and mildly increased in the CBS-/+, compared to WT mice PMID: 19021146
    43. Results collected from metabolome analyses suggested that CBS serves as a CO-sensitive modulator of H(2)S to support biliary excretion. PMID: 19085910
    44. hyperhomocysteinemia promotes differentiation of inflammatory monocyte subsets and their accumulation in atherosclerotic lesions via NAD(P)H oxidase-mediated oxidant stress in CBS deficiency PMID: 19858416

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Cysteine synthase/cystathionine beta-synthase family
  • 数据库链接：

    KEGG: mmu:12411

    STRING: 10090.ENSMUSP00000066878

    UniGene: Mm.206417