Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Cytochrome P450 4A10 (Cyp4a10)

Recombinant Mouse Cytochrome P450 4A10 (Cyp4a10)

  • 货号：
    CSB-YP530818MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP530818MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP530818MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP530818MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP530818MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cyp4a10
  • Uniprot No.：
  • 别名：
    Cyp4a10; Cyp4a-10Cytochrome P450 4A10; CYPIVA10; Cytochrome P450-LA-omega 1; Cytochrome P452; Lauric acid omega-hydroxylase; Long-chain fatty acid omega-monooxygenase; EC 1.14.14.80
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-509
  • 氨基酸序列
    MSVSALSPTR FADSLSGFLQ VASVLGLLLL LVKAVQFYLH RQWLLKAFQQ FPSPPFHWFF GHEKFKGDQE LQEIVSCIEN FPSAFPRWFW GSKAYLTVYD PDYMKVILGR SDPKANGAYR LLAPWIGYGL LLLNGQPWFQ HRRMLTPAFH YDILKPYVKN MADSIRLMLD KWERLADQDS SIEIFQHISL MTLDTVMKCA FSHKGSVQVD GNYRTYLQAI GDLNNLFHSR VRNIFHQNDT IYKLSSNGRL AKQACQLAHD HTDGVIKLRK DQLQDEGELE KIKKKRRLDF LDILLFARME NGDSMSDKDL RAEVDTFMFE GHDTTASGVS WIFYALATHP DHQQRCREEV QSLLGDGSSI TWDHLDQIPY TTMCIKEALR LYPPVPGIVR ELSTSVTFPD GRSLPKGVQV TLSIYGLHHN PKVWPNPEVF DPSRFAPDSP RHSHSFLPFS GGARNCIGKQ FAMSELKVIV ALTLLRFELL PDPTRVPMPL ARLVLKSKNG IYLHLKKLH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of long-chain fatty acids. Acts as a major omega-hydroxylase for dodecanoic (lauric) acid in liver. In kidney, may play an important role in omega-hydroxylation of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) to 20-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure. Also participates in the formation of anti-inflammatory hydroxyepoxyeicosatrienoic acids (HEETs) in kidney by converting 8,9-epoxyeicosatrienoic acid (EET) to 20,8,9-HEET, an activator of PPARA. Displays substantially lower fatty acid omega-1 hydroxylase activity. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
  • 基因功能参考文献：
    1. These results implicate Cyp4a10 and Cyp4a14 in the regulation of the host inflammatory response to enteropathogenic bacterial infection but not to acute aseptic inflammation. PMID: 24413902
    2. High salt triggers possible A(2A)AR-induced relaxation through CYP epoxygenase in both eNOS(+/+) and eNOS(-/-). PMID: 21161333
    3. Cytochromes P450 4A metabolize arachidonic acid into hydroxyeicosatetraenoic acids (HETEs) that exhibit potent actions on airway smooth muscle tone. p. 181 PMID: 15066132
    4. Results show that disruption of the murine cytochrome P450, family 4, subfamily a, polypeptide 10 (Cyp4a10) gene causes a type of hypertension that is, like most human hypertension, dietary salt sensitive. PMID: 16691295
    5. Role of CYP4A in reactive oxygen species production ad apoptosis of podocytes in a mouse model of type 1 diabetes. PMID: 19208908

    显示更多

    收起更多

  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 组织特异性：
    Highly expressed in the kidneys of both genders.
  • 数据库链接：

    KEGG: mmu:13117

    STRING: 10090.ENSMUSP00000061126

    UniGene: Mm.10742