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  4. Recombinant Mouse Cytochrome P450 4A14 (Cyp4a14)

Recombinant Mouse Cytochrome P450 4A14 (Cyp4a14)

  • 货号：
    CSB-YP518576MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP518576MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP518576MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP518576MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP518576MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cyp4a14
  • Uniprot No.：
  • 别名：
    Cyp4a14Cytochrome P450 4A14; CYPIVA14; Lauric acid hydroxylase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    5-507
  • 氨基酸序列
    LFSPTR YLDGISGFFQ WAFLLSLFLV LFKAVQFYLR RQWLLKTLQH FPCMPSHWLW GHHLKDKELQ QILIWVEKFP SACLQCLSGS NIRVLLYDPD YVKVVLGRSD PKASGIYQFF APWIGYGLLL LNGKKWFQHR RMLTPAFHYD ILKPYVKIMA DSVNIMLDKW EKLDGQDHPL EIFHCVSLMT LDTVMKCAFS YQGSVQLDEN SKLYTKAVED LNNLTFFRLR NAFYKYNIIY NMSSDGRLSH HACQIAHEHT DGVIKMRKSQ LQNEEELQKA RKKRHLDFLD ILLFARMEDR NSLSDEDLRA EVDTFMFEGH DTTASGISWI FYALATHPEH QQRCREEVQS ILGDGTSVTW DHLGQMPYTT MCIKEALRLY PPVISVSREL SSPVTFPDGR SIPKGITATI SIYGLHHNPR FWPNPKVFDP SRFAPDSSHH SHAYLPFSGG SRNCIGKQFA MNELKVAVAL TLLRFELLPD PTRIPVPIAR LVLKSKNGIH LCLKKLR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of saturated carbon hydrogen bonds of fatty acids. May act as a major omega- and ommega-1 hydroxylase for dodecanoic (lauric) acid in kidney. Catalyzes with low efficiency the epoxidation of 11,12-double bond of arachidonic acid.
  • 基因功能参考文献：
    1. CYP4A14 may represent a useful target for the treatment of AngII-associated renal damage. PMID: 29277328
    2. this study provides a link between CYP4A14 and NAFLD. Overexpression of CYP4A14 promotes, whereas gene disruption of CYP4A14 attenuates, HFD- and MCD-induced liver lipid accumulation. Ablation of CYP4A14 gene markedly attenuates liver damage, inflammation, and fibrosis in MCD-induced NASH. PMID: 28270609
    3. Hypertension in Cyp4a14(-/-) male mice was accompanied by decreased renal perfusion and reduced glomerular filtration rates PMID: 28912346
    4. DEHP exposure did not influence lipase activity, whereas it slightly enhanced UGT activity and exclusively increased CYP4A14 levels in pregnant and/or postpartum dams. PMID: 22159897
    5. Deletion of the Cyp4a gene conferred a prohypertensive status via increased 20-HETE synthesis and eNOS uncoupling leading to increased oxidative stress, enhanced vasoconstriction but diminished vasodilation as well as a defect in renal excretory capacity. PMID: 20943934
    6. Hypertension in the Cyp4a14 knockout mice appears to be driven by excessive fluid reabsorption in the proximal tubule, which is secondary to overexpression of NHE3. PMID: 19713718
    7. Drug metabolizing enzyme induction of Cyp4a14 is reported in experimental fatty liver. PMID: 18488193

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 组织特异性：
    Gender- and strain-specific expression in kidney (at protein level). Predominantly expressed in females, the expression among strains decreasing in the following order: FVB/N > NMRI > Balb/c > 129 Sv/J > C57BL/6. Expressed at very low level in liver, kidn
  • 数据库链接：

    KEGG: mmu:13119

    STRING: 10090.ENSMUSP00000030487

    UniGene: Mm.250901