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  4. Recombinant Mouse Cytokine-inducible SH2-containing protein (Cish)

Recombinant Mouse Cytokine-inducible SH2-containing protein (Cish)

  • 货号：
    CSB-YP720292MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP720292MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP720292MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP720292MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP720292MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cish; CisCytokine-inducible SH2-containing protein; CIS; CIS-1; Suppressor of cytokine signaling; SOCS
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-257
  • 氨基酸序列
    MVLCVQGSCP LLAVEQIGRR PLWAQSLELP GPAMQPLPTG AFPEEVTEET PVQAENEPKV LDPEGDLLCI AKTFSYLRES GWYWGSITAS EARQHLQKMP EGTFLVRDST HPSYLFTLSV KTTRGPTNVR IEYADSSFRL DSNCLSRPRI LAFPDVVSLV QHYVASCAAD TRSDSPDPAP TPALPMSKQD APSDSVLPIP VATAVHLKLV QPFVRRSSAR SLQHLCRLVI NRLVADVDCL PLPRRMADYL RQYPFQL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • 基因功能参考文献：
    1. CISH mediates control of M. tuberculosis in mice early after infection via regulation of innate immune mechanisms. PMID: 29050767
    2. CIS interacted with phosphorylated EpoR at Y401, which was critical for the activation of STAT5 and ERK. PMID: 28038963
    3. this study identifies CIS as a critical negative regulator of IL-15 signaling in NK cells PMID: 27213690
    4. Data show that cytokine inducible SH2-containing protein Cish is induced upon T cell receptor stimulation and in the tumor microenvironment. PMID: 26527801
    5. Results show that CISH has no non-redundant functions in beta cell proliferation or glucose homeostasis during pregnancy in mice. Socs2 might compensate for the loss of Cish during pregnancy. PMID: 23949579
    6. CIS has a critical role in controlling the proallergic generation of helper T cells. PMID: 23727894
    7. CISH expression at the later stage of dendritic cell development triggers the shutdown of DC progenitor cell proliferation and facilitates DC differentiation into a potent stimulator of CTLs. PMID: 22002016
    8. CIS and SOCS3 play a role as negative feedback inhibitors of PRL action; Inhibition of CIS and SOCS3 expression by glucocorticoids contributes to the positive effect of glucocorticoids on PRL-induced STAT5 activation. PMID: 12089360
    9. Induction of CIS in response to EPO stimulation depends on Stat5 but not MAP kinase or PI 3-kinase. Overexpression negatively regulates EPO-mediated cell proliferation, Stat5 phosphorylation, & activation of a Stat-dependent luciferase reporter. PMID: 12396724
    10. CIS negatively regulates signaling at two levels, apoptosis and proliferation, and thereby sets a threshold for signal transduction PMID: 12441334
    11. IL-6 inhibits hepatic GH signaling by upregulating Cis and Socs-3, which may involve activation of STAT3. PMID: 12519742
    12. histone acetylation and chromatin remodeling events occurring during transcriptional activation of the endogenous murine Cis gene, a STAT5 target gene PMID: 14627821
    13. The desensitization of the JAK2/STAT5b GH signaling pathway observed in pregnant mice would then be mainly related to increased CIS levels rather than to the other regulatory proteins examined. PMID: 15899943
    14. C-terminal part of the CIS SOCS-box is required for functional interaction with the cytokine receptor motifs examined. PMID: 16961462
    15. Elongin B/C recruitment regulates substrate binding by CIS PMID: 18508766

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  • 组织特异性：
    Expressed in kidney, lung and liver. Detected to a lower extent in stomach and heart.
  • 数据库链接：

    KEGG: mmu:12700

    STRING: 10090.ENSMUSP00000082183

    UniGene: Mm.4592