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  4. Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 1 (Cpeb1)

Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 1 (Cpeb1)

  • 中文名称：
    Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 1(Cpeb1),Yeast
  • 货号：
    CSB-YP005887MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 1(Cpeb1),Yeast
  • 货号：
    CSB-EP005887MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 1(Cpeb1),Yeast
  • 货号：
    CSB-EP005887MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 1(Cpeb1),Yeast
  • 货号：
    CSB-BP005887MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytoplasmic polyadenylation element-binding protein 1(Cpeb1),Yeast
  • 货号：
    CSB-MP005887MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cpeb1
  • Uniprot No.：
  • 别名：
    Cpeb1; CpebCytoplasmic polyadenylation element-binding protein 1; CPE-BP1; CPE-binding protein 1; mCPEB; mCPEB-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-561
  • 氨基酸序列
    MAFSLEEAAG RIKDCWDNQE VPALSTCSNA NIFRRINAIL DDSLDFSKVC TTPINRGIHD QLPDFQDSEE TVTSRMLFPT SAQESPRGLP DANGLCLGLQ SLSLTGWDRP WSTQDSDSSA QSSTQSVLSM LQNPLGNVLG KAPLSFLSLD PLGSDLDKFP APSVRGSRLD TRPILDSRSS SPSDSDTSGF SSGSDHLSDL ISSLRISPPL PFLSMTGNGP RDPLKMGVGS RMDQEQAALA AVAPSPTSAP KRWPGASVWP SWDLLGAPKD PFSIEREARL HRQAAAVNEA TCTWSGQLPP RNYKNPIYSC KVFLGGVPWD ITEAGLVNTF RVFGSLSVEW PGKDGKHPRC PPKGNMPKGY VYLVFELEKS VRALLQACSH DPLSPDGLSE YYFKMSSRRM RCKEVQVIPW VLADSNFVWS PSQRLDPSRT VFVGALHGML NAEALAAILN DLFGGVVYAG IDTDKHKYPI GSGRVTFNNQ RSYLKAVTAA FVEIKTTKFT KKVQIDPYLE DSLCLICSSQ PGPFFCRDQV CFKYFCRSCW HWRHSMEGLR HHSPLMRNQK N
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the 3'-UTR of mRNAs. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation. Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses. Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation. Induces the assembly of stress granules in the absence of stress. Required for cell cycle progression, specifically for prophase entry.
  • 基因功能参考文献：
    1. CPEB1 level was increased in the basolateral amygdala (BLA) of model mice injected with Complete Freund's Adjuvant. CPEB1 knockdown in the BLA can relieve anxiety-like behaviors but not pain-like behaviors by heightening inhibitory transmission and restoring the excitatory/inhibitory balance in model mice. PMID: 29242136
    2. CPEB1 regulation of MMP9 mRNA expression mediates metastasis of breast cancer cells PMID: 26411364
    3. DAZL and CPEB1 regulate mRNA translation during oocyte maturation. PMID: 26826184
    4. Identify a mechanism of VEGF overexpression in liver and mesentery that promotes pathologic, but not physiologic, angiogenesis, via sequential and nonredundant functions of CPEB1 and CPEB4. PMID: 26627607
    5. cytoplasmic-element-binding control of transforming growth factor beta-activated kinase 1 mRNA translation mediates the inflammatory immune response PMID: 25452303
    6. Myoepithelial cells prevent EMT by influencing the polarity and proliferation of luminal epithelial cells in a mechanism that requires translational silencing of myoepithelial Twist1 by CPEB1. PMID: 24634508
    7. Fmr1(-/y); Cpeb1(-/-) double-knockout mice displayed amelioration of biochemical, morphological, electrophysiological and behavioral phenotypes associated with fragile X syndrome. PMID: 24141422
    8. poly(A) polymerase Gld2, deadenylase PARN, and translation inhibitory factor neuroguidin (Ngd) are components of a dendritic CPEB-associated polyadenylation apparatus PMID: 22727665
    9. CPEB-mediated zonal occludens-1 mRNA localization is essential for tight-junction assembly and mammary epithelial cell polarity PMID: 22334078
    10. when the Cpeb1 knockout mice were fed a high-fat diet, their livers became insulin-resistant.Our results show that Cpeb1 regulated translation is a key process involved in insulin signaling PMID: 22253608
    11. CPEB deficiency not only increases IL-6 production but also renders the cell incapable of a senescence-promoting response. PMID: 21536657
    12. Data suggest that KHDC1B, via its interaction with mCEPB1, may regulate translation of mRNA targets required for oocyte maturation. PMID: 20668163
    13. CPEB undergoes Thr 171 phosphorylation at E16.5, but dephosphorylation at the E18.5, when most oocytes are entering diplotene PMID: 12815066
    14. Data suggest that CPEB-1 contributes in the translational control of mRNAs that is critical only for some selected forms of long-term potentiation and long-term depression. PMID: 15169862
    15. These results demonstrate that CPEB phosphorylation and translation are regulated by CaMKII activity and provide a possible mechanism for how dendritic protein synthesis in the hippocampus may be stimulated during synaptic plasticity PMID: 15175389
    16. CPEB binds the small intracellular domain (ICD) of APLP1. PMID: 16314516
    17. a new eukaryotic initiation factor 4E (eIF4E) binding protein, Neuroguidin (Ngd), and its interaction with CPEB is described. PMID: 16705177
    18. CPEB knockout mouse is sterile but overtly normal, but embryo fibroblasts derived from this mouse (MEF) do not enter senescence in culture as do wild-type MEFs, but instead are immortal. PMID: 17015432
    19. CPEB controls the expression of Gdf9 mRNA, which in turn is necessary for oocyte-follicle development. PMID: 17050619
    20. CPEB-1 control of c-Jun mRNA translation regulates GH gene expression and resulting downstream signaling events (e.g., synaptic plasticity) in the mouse hippocampus. PMID: 18716208
    21. Data identified an interaction of CPEB (cytoplasmic polyadenylation-element-binding protein) 1 and CPEB2 with the 3'-UTR (untranslated region) of HIF-1alpha mRNA. PMID: 18752464

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  • 亚细胞定位：
    Cytoplasm. Cell junction, synapse. Cytoplasm, P-body. Cytoplasmic granule. Membrane. Cell junction, synapse, postsynaptic density.
  • 蛋白家族：
    RRM CPEB family
  • 组织特异性：
    Expressed in hippocampus, cerebral cortex and oocytes (at protein level). Expressed in brain, heart, kidney, lung and ovary and testis. Weakly expressed in granular cells of dentate gyrus and the pyramidal cells of CA3 and CA1 of the hippocampus.
  • 数据库链接：

    KEGG: mmu:12877

    STRING: 10090.ENSMUSP00000137079

    UniGene: Mm.273122