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  4. Recombinant Mouse D-3-phosphoglycerate dehydrogenase (Phgdh)

Recombinant Mouse D-3-phosphoglycerate dehydrogenase (Phgdh)

  • 中文名称：
    小鼠Phgdh重组蛋白
  • 货号：
    CSB-YP717226MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Phgdh重组蛋白
  • 货号：
    CSB-EP717226MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Phgdh重组蛋白
  • 货号：
    CSB-EP717226MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Phgdh重组蛋白
  • 货号：
    CSB-BP717226MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Phgdh重组蛋白
  • 货号：
    CSB-MP717226MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Phgdh
  • Uniprot No.：
  • 别名：
    Phgdh; D-3-phosphoglycerate dehydrogenase; 3-PGDH; EC 1.1.1.95; A10
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-533
  • 氨基酸序列
    AFANLRKVL ISDSLDPCCR KILQDGGLQV VEKQNLSKEE LIAELQDCEG LIVRSATKVT ADVINAAEKL QVVGRAGTGV DNVDLEAATR KGILVMNTPN GNSLSAAELT CGMIMCLARQ IPQATASMKD GKWDRKKFMG TELNGKTLGI LGLGRIGREV ATRMQSFGMK TVGYDPIISP EVAASFGVQQ LPLEEIWPLC DFITVHTPLL PSTTGLLNDS TFAQCKKGVR VVNCARGGIV DEGALLRALQ SGQCAGAALD VFTEEPPRDR ALVDHENVIS CPHLGASTKE AQSRCGEEIA VQFVDMVKGK SLTGVVNAQA LTSAFSPHTK PWIGLAEAMG TLMHAWAGSP KGTIQVVTQG TSLKNAGTCL SPAVIVGLLR EASKQADVNL VNAKLLVKEA GLNVTTSHNP GVPGEQGSGE CLLTVALAGA PYQAVGLVQG TTPMLQMLNG AVFRPEVPLR RGQPLLVFRA QPSDPGMLPT MIGLLAEAGV QLLSYQTSMV SDGEPWHVMG LSSLLPSLET WKQHVLEAFQ FCF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Does not catalyze the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.
  • 基因功能参考文献：
    1. These results indicate that the PHGDH gene expression, dictated by IL-2R signaling, is a crucial event for DNA synthesis during S phase of activated T cells. PMID: 24434753
    2. Using transgenic mice expressing enhanced GFP under the Srr promoter and mice with targeted deletion of Srr or 3-Phosphoglycerate dehydrogenase, we demonstrate predominantly neuronal sources of d-serine dependent on astrocytic supply of l-serine. PMID: 23884950
    3. Elevated levels of 4E-BP1 may be involved in a mechanism to arrest brain development in Phgdh knock out embryos. PMID: 23350942
    4. Phgdh deletion reveals a pivotal role for L-serine biosynthesis in controlling the level of brain D-serine, an N-methyl-D-aspartate receptor co-agonist PMID: 20966073
    5. Data suggest expression of Phgdh in endometrium is regulated by Hoxa10. PMID: 19778996
    6. the Phgdh-dependent phosphorylated pathway is essential for normal embryonic development, especially for brain morphogenesis PMID: 14645240
    7. the presence of cis-acting elements confer the cell type specificity of Phgdh transcription in the distal promoter region. PMID: 15139021
    8. The cis-acting elements necessary for basal transcription of Phgdh are contained within the -196/+4 proximal sequence of the promoter, in which the conserved Sp1 recognition sites play an important role for basal promoter activity. PMID: 15256251
    9. either Phgdh or ASCT1 is provided to each segment of renal tubules, suggesting that metabolic interplay mediated by L-serine biosynthesis and supply may exist in the kidney PMID: 17510490
    10. Inactivation of Phgdh gene with changes in gene expression and associated regulatory networks resulting from serine deficiency are reported. PMID: 18228065
    11. Results demonstrate injury-induced changes in Phgdh and ASCT1 expression. PMID: 18751891
    12. normal cell cycle progression and subsequent neurogenesis of radial glia cells are severely impaired by serine deficiency PMID: 19114063
    13. demonstrated expression of Phgdh was upregulated in subventricular zone; expression was selective to cells having features of astrocyte-like primary neural stem cells & their progeny; Phgdh expression was virtually absent in committed neuronal precursors PMID: 19429008
    14. The mouse Phgdh gene spans approximately 27 kilobases in length and comprises 12 exons with 11 intervening introns and is mapped to chromosome 3, region F2-F3 by FISH. PMID: 15256251

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  • 蛋白家族：
    D-isomer specific 2-hydroxyacid dehydrogenase family
  • 数据库链接：

    KEGG: mmu:236539

    STRING: 10090.ENSMUSP00000064755

    UniGene: Mm.16898