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  4. Recombinant Mouse DCC-interacting protein 13-beta (Appl2)

Recombinant Mouse DCC-interacting protein 13-beta (Appl2)

  • 货号：
    CSB-YP812988MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP812988MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP812988MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP812988MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP812988MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Appl2
  • Uniprot No.：
  • 别名：
    Appl2; Dip13b; Dip3bDCC-interacting protein 13-beta; Dip13-beta; Adapter protein containing PH domain; PTB domain and leucine zipper motif 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-662
  • 氨基酸序列
    MPAVDKLLLE EALQDSPQAR SLLSVFEEDA GTLTDYTNQL LQAMQRVYGA QNEMCLATQQ LSRQLLAYEK QNFALGKGDE EVISTLHYFS KVMDELNGLH TELAKQLADT MVLPVIQFRE KDLTEVSTLK DLFGLASSEH DLSMAKYSRL PKKKENEKAK TEIVKEVAAA RRKQHLSSLQ YYCALNALQY RKRAAMMEPL IGFAHGQINF FKRGAEMFSK SMDGFLSSVK DMVQSIQVEL EAEADKMRVS QQELLSVSES VYTPDIDVAT AQINRNLIQK TGYLNLRNKT GLVTTTWERL YFFTQGGNLM CQPRGAVAGG LIQDLDNCSV MAVDCEDRRY CFQISTPSGK PGIILQAESR KEYEEWICAV NNISRQIYLT DNPEAVAIKL NQTALQAVTP ITSFGKKQES SCSSQNIKNS DIEDDNIVPK ATASIPETEE LIAPGTPIQF DIVLPATEFL DQNRGGRRTN PFGETEDGSF PEAEDSLLQQ MFIVRFLGSM AVKTDSTAEV IYEAMRQVLA ARAIHNIFRM TESHLMVTSQ TLRLIDPQTQ VSRACFELTS VTQFAAHQEN KRLVGFVIRV PESTGEESLS TYIFESNSEG EKICYAINLG KEIIEVQKDP EALARLMLSV PLTNDGKYVL LNDQADDTGG SPSENRGAES EA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex. Plays a role in immune response by modulating phagocytosis, inflammatory and innate immune responses. In macrophages, enhances Fc-gamma receptor-mediated phagocytosis through interaction with RAB31 leading to activation of PI3K/Akt signaling. In response to LPS, modulates inflammatory responses by playing a key role on the regulation of TLR4 signaling and in the nuclear translocation of RELA/NF-kappa-B p65 and the secretion of pro- and anti-inflammatory cytokines. Also functions as a negative regulator of innate immune response via inhibition of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1. Plays a role in endosomal trafficking of TGFBR1 from the endosomes to the nucleus. plays a role in cell metabolism by regulating adiponecting ans insulin signaling pathways and adaptative thermogenesis. In muscle, negatively regulates adiponectin-simulated glucose uptake and fatty acid oxidation by inhibiting adiponectin signaling pathway through APPL1 sequestration thereby antagonizing APPL1 action. In muscles, negativeliy regulates insulin-induced plasma membrane recruitment of GLUT4 and glucose uptake through interaction with TBC1D1. Plays a role in cold and diet-induced adaptive thermogenesis by activating ventromedial hypothalamus (VMH) neurons throught AMPK inhibition which enhances sympathetic outflow to subcutaneous white adipose tissue (sWAT), sWAT beiging and cold tolerance. Also plays a role in other signaling pathways namely Wnt/beta-catenin, HGF and glucocorticoid receptor signaling. Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex. May affect adult neurogenesis in hippocampus and olfactory system via regulating the sensitivity of glucocorticoid receptor. Required for fibroblast migration through HGF cell signaling.
  • 亚细胞定位：
    Early endosome membrane; Peripheral membrane protein. Nucleus. Cell membrane. Endosome membrane. Cytoplasm. Cytoplasmic vesicle, phagosome. Cell projection, ruffle. Cell projection, ruffle membrane. Cell membrane. Cytoplasmic vesicle, phagosome membrane.
  • 组织特异性：
    Expressed in insulin-target tissues including skeletal muscle, liver, fat, and brain. Highly expressed in kidney and pancreas. Abundantly expressed in the ventromedial hypothalamus (VMH), barely detectable in the arcuate nucleus (ARC) and paraventricular
  • 数据库链接：

    KEGG: mmu:216190

    STRING: 10090.ENSMUSP00000020500

    UniGene: Mm.282985