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  4. Recombinant Mouse DDRGK domain-containing protein 1 (Ddrgk1)

Recombinant Mouse DDRGK domain-containing protein 1 (Ddrgk1)

  • 中文名称：
    Recombinant Mouse DDRGK domain-containing protein 1(Ddrgk1),Yeast
  • 货号：
    CSB-YP802245MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse DDRGK domain-containing protein 1(Ddrgk1),Yeast
  • 货号：
    CSB-EP802245MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse DDRGK domain-containing protein 1(Ddrgk1),Yeast
  • 货号：
    CSB-EP802245MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse DDRGK domain-containing protein 1(Ddrgk1),Yeast
  • 货号：
    CSB-BP802245MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse DDRGK domain-containing protein 1(Ddrgk1),Yeast
  • 货号：
    CSB-MP802245MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ddrgk1
  • Uniprot No.：
  • 别名：
    Ddrgk1; Ufbp1DDRGK domain-containing protein 1; UFM1-binding and PCI domain-containing protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    29-315
  • 氨基酸序列
    AA ADGEPLHNEE ERAGAGQVGR SLPQESEEQR TGSRPRRRRD LGSRLQAQRR AQRVAWEDGD ENVGQTVIPA QEEEGIEKPA EVHPTGKIGA KKLRKLEEKQ ARKAQREAEE AEREERKRLE SQREAEWKKE EERLRLKEEQ KEEEERKAQE EQARREHEEY LKLKEAFVVE EEGVSETMTE EQSHSFLTEF INYIKKSKVV LLEDLAFQMG LRTQDAINRI QDLLTEGTLT GVIDDRGKFI YITPEELAAV ANFIRQRGRV SITELAQASN SLISWGQDLP AQASA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Substrate adapter for ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, which plays a key role in reticulophagy (also called ER-phagy). In response to endoplasmic reticulum stress, promotes recruitment of the E3 UFM1-protein ligase UFL1 to the endoplasmic reticulum membrane: in turn, UFL1 mediates ufmylation of proteins such as RPN1 and RPL26/uL24, promoting reticulophagy of endoplasmic reticulum sheets. Ufmylation-dependent reticulophagy inhibits the unfolded protein response (UPR) by regulating ERN1/IRE1-alpha stability. Ufmylation in response to endoplasmic reticulum stress is essential for processes such as hematopoiesis or inflammatory response. Required for TRIP4 ufmylation, thereby regulating nuclear receptors-mediated transcription. May play a role in NF-kappa-B-mediated transcription through regulation of the phosphorylation and the degradation of NFKBIA, the inhibitor of NF-kappa-B. Plays a role in cartilage development through SOX9, inhibiting the ubiquitin-mediated proteasomal degradation of this transcriptional regulator.
  • 基因功能参考文献：
    1. Study results provide evidence that DDRGK1 is essential for endoplasmic reticulum (ER) homeostasis regulation in both human cancer cells and mouse hematopoietic stem cells. Depletion of DDRGK1 activates apoptotic pathway by targeting the ER-stress sensor IRE1alpha. DDRGK1 regulates IRE1alpha protein stability via its interaction with the kinase domain of IRE1alpha. PMID: 28128204
    2. findings suggest that UFBP1, along with ASC1 and other ufmylation components, play pleiotropic roles in regulation of hematopoietic cell survival and differentiation via modulating ER homeostasis and erythroid lineage-specific gene expression PMID: 26544067
    3. These data suggest that UFM1-UFBP1 participate in preventing endoplasmic reticulum stress-induced apoptosis in protein secretory cells. PMID: 21494687
  • 亚细胞定位：
    Endoplasmic reticulum. Endoplasmic reticulum membrane.
  • 蛋白家族：
    DDRGK1 family
  • 组织特异性：
    Ubiquitously expressed. Higher expression in pancreatic islets, pancreatic acini and testis (at protein level). Highly expressed in the intestinal exocrine cells.
  • 数据库链接：

    KEGG: mmu:77006

    STRING: 10090.ENSMUSP00000086988

    UniGene: Mm.440063