Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse DEP domain-containing mTOR-interacting protein (Deptor)

Recombinant Mouse DEP domain-containing mTOR-interacting protein (Deptor)

  • 货号：
    CSB-YP687983MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP687983MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP687983MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP687983MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP687983MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Deptor; Depdc6; Kiaa4200; DEP domain-containing mTOR-interacting protein; DEP domain-containing protein 6
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-409
  • 氨基酸序列
    MEEGSSGGSG SSDSNAGGSG GVQQRELERM AEVLVTGEQL RLRLHEEKVI KDRRHHLKTY PNCFVAKELI DWLIEHKEAS DRETAIKLMQ KLADRGIIHH VCDEHKEFKD VKLFYRFRKD DGTFALDSEV KAFMRGQRLY EKLMSPETTL LQPREEEGVK YERTFMASEF LDWLVQEGEA TTRKEAEQLC HRLMDHGIIQ HVSNKHPFVD SNLLYQFRMN FRRRRRLMEL LNETSPSSQE THDSPFCLRK QSHDSRKSTS FMSVSPSKEI KIVSAVRRSS MSSCGSSGYF SSSPTLSSSP PVLCNPKSVL KRPVTSEELL TPGAPYARKT FTIVGDAVGW GFVVRGSKPC HIQAVDPSGP AAAAGMKVCQ FVVSVNGLNV LNVDYRTVSN LILTGPRTIV MEVMEELDC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.
  • 基因功能参考文献：
    1. Hepatic DEPTOR accelerates the inhibition of mTORC1 during the transition to fasting to adjust metabolism to the nutritional status. PMID: 28462079
    2. Cul-1 deneddylation led to Deptor accumulation and subsequent mTOR inactivation, which had an inhibitory effect on dendritic cells in mouse model of inflammatory bowel disease. PMID: 27224922
    3. These results support the idea that other neuronal populations are responsible for these phenotypes. Nonetheless, we observed a mild elevation in fasting blood glucose, insulin resistance, and alterations in liver glucose and lipid homeostasis in mice overexpressing DEPTOR in POMC neurons. Taken together, these results show that DEPTOR overexpression in POMC neurons does not affect energy balance regulation but could modu PMID: 27097662
    4. The present study indicates that regulation of DEPTOR/mTOR signaling may be an important mechanism for glutamine in prevention against the development of colitis-associated colorectal cancer (CAC): the chemopreventive effect of dietary glutamine on CAC is, at least in part, associated with the induction of autophagy. PMID: 27144580
    5. DEPTOR regulates liver inflammation at least partially via mTORC1 pathway, and is down-regulated by lypopolysaccharides through p65. PMID: 27179948
    6. p38gamma and p38delta control heart growth by modulating mTOR pathway through DEPTOR phosphorylation and subsequent degradation. PMID: 26795633
    7. DEPTOR plays a key role in maintaining stem cell pluripotency by limiting mTOR activity in undifferentiated embryonic stem cells PMID: 25258312
    8. DEPTOR is induced by glucocorticoids during adipogenesis and that its overexpression promotes, while its suppression blocks, adipogenesis. PMID: 22883231
    9. Data show that knockdown reduced Deptor mRNA and protein content by 90%, which increased phosphorylation of mTOR kinase substrates, 4E-BP1 and S6K1, and concomitantly increased protein synthesis. PMID: 21607293

    显示更多

    收起更多

  • 数据库链接：

    KEGG: mmu:97998

    STRING: 10090.ENSMUSP00000094167

    UniGene: Mm.295397