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  4. Recombinant Mouse DNA-directed DNA/RNA polymerase mu (Polm)

Recombinant Mouse DNA-directed DNA/RNA polymerase mu (Polm)

  • 中文名称：
    小鼠Polm重组蛋白
  • 货号：
    CSB-YP864330MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Polm重组蛋白
  • 货号：
    CSB-EP864330MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Polm重组蛋白
  • 货号：
    CSB-EP864330MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Polm重组蛋白
  • 货号：
    CSB-BP864330MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Polm重组蛋白
  • 货号：
    CSB-MP864330MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Polm
  • Uniprot No.：
  • 别名：
    Polm; polmu; DNA-directed DNA/RNA polymerase mu; Pol Mu; EC 2.7.7.7; Terminal transferase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-496
  • 氨基酸序列
    MLPKRRRVRA GSPHSAVASS TPPSVVRFPD VAIYLAEPRM GRSRRAFLTR LARSKGFRVL DAYSSKVTHV VMEGTSAKEA ICWQKNMDAL PTGCPQPALL DISWFTESMA AGQPVPEEGR HHLEVAEPRK EPPVSASMPA YACQRPSPLT HHNTLLSEAL ETLAEAAGFE ANEGRLLSFS RAASVLKSLP CPVASLSQLH GLPYFGEHST RVIQELLEHG TCEEVKQVRC SERYQTMKLF TQVFGVGVKT ANRWYQEGLR TLDELREQPQ RLTQQQKAGL QYYQDLSTPV RRADAEALQQ LIEAAVRQTL PGATVTLTGG FRRGKLQGHD VDFLITHPEE GQEVGLLPKV MSCLQSQGLV LYHQYHRSHL ADSAHNLRQR SSTMDAFERS FCILGLPQPQ QAALAGALPP CPTWKAVRVD LVVTPSSQFP FALLGWTGSQ FFERELRRFS RQEKGLWLNS HGLFDPEQKR VFHATSEEDV FRLLGLKYLP PEQRNA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.
  • 基因功能参考文献：
    1. Findings demonstrate that Polmu is necessary for proper retinal development, and support that the generation of DNA double-strand breaks and their repair via the non-homologous end-joining pathway are genuine processes involved in neural development. PMID: 27172884
    2. A role for PolMU in the prevention of sarcomagenesis on a murine P53-deficient background was identified. PMID: 28460268
    3. Pol mu and Pol lambda play a key role in conferring on NHEJ the flexibility required for accurate and efficient repair PMID: 26240371
    4. Polmu deficiency activates transcriptional networks that reduce constitutive apoptosis, leading to enhanced liver repair at old age. PMID: 24691161
    5. DNA polymerase mu has a role in decreased learning and brain long-term potentiation in aged mice PMID: 23301049
    6. These data point to a critical role for pol mu as a global repair player that increases the efficiency and the fidelity of non-homologous end-joining. PMID: 22071146
    7. pronounced senescence in pololymerase mu deficient cells PMID: 20947452
    8. A series of molecular-dynamics simulations with and without the incoming nucleotide in various forms, including mutant systems, based on pol mu's crystal ternary structure, were analyzed. PMID: 21081096
    9. Pol mu is not required for normal Ig gene hypermutation. PMID: 11937519
    10. Pol mu promotes accuracy during Ig kappa recombination. PMID: 16061182
    11. third hypervariable region assumes for each immunoglobulin chain, with pol lambda maintaining a large heavy chain junctional heterogeneity and pol mu ensuring a restricted light chain junctional variability PMID: 16860755
    12. The crystal structure of the polymerase domain of murine Pol mu bound to gapped DNA with a correct dNTP at the active site reveals substrate interactions with side chains in Pol mu that differ from other family X members. PMID: 17159995
    13. Polmu participates in the repair of early-embryo, RAG-induced double-strand breaks and subsequently may contribute to preserve the genomic stability and cellular homeostasis of lymphohematopoietic precursors during development PMID: 19103746
    14. Polm is required for hematopoietic development with an role in maintaining genetic stability in hematopoietic and non-hematopoietic tissues. PMID: 19229323

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase type-X family
  • 数据库链接：

    KEGG: mmu:54125

    STRING: 10090.ENSMUSP00000020767

    UniGene: Mm.260194