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  4. Recombinant Mouse DNA repair protein RAD51 homolog 4 (Rad51d)

Recombinant Mouse DNA repair protein RAD51 homolog 4 (Rad51d)

  • 货号：
    CSB-YP019268MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019268MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019268MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019268MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019268MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rad51d
  • Uniprot No.：
  • 别名：
    Rad51d; R51h3; Rad51l3; DNA repair protein RAD51 homolog 4; R51H3; RAD51 homolog D; RAD51-like protein 3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-329
  • 氨基酸序列
    MGMLRAGLCP GLTEETVQLL RGRKIKTVAD LAAADLEEVA QKCGLSYKAL VALRRVLLAQ FSAFPLNGAD LYEELKTSTA ILSTGIGSLD KLLDAGLYTG EVTEIVGGPG SGKTQVCLCV AANVAHSLQQ NVLYVDSNGG MTASRLLQLL QARTQDEEKQ ASALQRIQVV RSFDIFRMLD MLQDLRGTIA QQEATSSGAV KVVIVDSVTA VVAPLLGGQQ REGLALMMQL ARELKILARD LGVAVVVTNH LTRDWDGRRF KPALGRSWSF VPSTRILLDV TEGAGTLGSS QRTVCLTKSP RQPTGLQEMI DIGTLGTEEQ SPELPGKQT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.
  • 基因功能参考文献：
    1. The role of the RAD51D HR protein during thiopurine induction of mitotic catastrophe. PMID: 28945288
    2. RNF138 is a newly identified regulatory component of the HR mediated DNA repair pathway that has implications toward understanding how ubiquitination modifies the functions of the RAD51 paralog protein complex PMID: 27161866
    3. Cells deficient in RAD51D (a RAD51 paralogue) are extremely sensitive to 6-thioguanine. PMID: 21205838
    4. RAD51D protects against MLH1-dependent cytotoxic responses to O(6)-methylguanine PMID: 20133210
    5. a fragment of Rad51B containing amino acid residues 1-75 interacts with the C-terminus and linker of Rad51C, residues 79-376, and this region of Rad51C also interacts with mRad51D and Xrcc3 PMID: 14704354
    6. Findings support a crucial role for mammalian RAD51D in normal development, recombination, and maintaining mammalian genome stability. PMID: 15781618
    7. These cell-based studies are the first evidence that ATP binding and hydrolysis by RAD51D are required for efficient HR repair of DNA interstrand crosslinks. PMID: 16236763
    8. Rad51d alternative splice variants potentially modulate mechanisms of homologous recombination by sequestering either RAD51C or XRCC2 PMID: 19327148
    9. Interactions of four candidate proteins (SFPQ, NONO, MSH2 and mini chromosome maintenance protein 2) were confirmed by Western blot analysis of co-precipitation eluates and were also verified to form ex vivo complexes with RAD51D. PMID: 19658102

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  • 亚细胞定位：
    Nucleus. Chromosome, telomere.
  • 蛋白家族：
    RecA family, RAD51 subfamily
  • 组织特异性：
    Highly expressed in brain followed by testis. Also expressed in heart, liver, kidney, spleen, lung and skeletal muscle.
  • 数据库链接：

    KEGG: mmu:19364

    STRING: 10090.ENSMUSP00000018985

    UniGene: Mm.441571