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  4. Recombinant Mouse Death-associated protein kinase 2 (Dapk2)

Recombinant Mouse Death-associated protein kinase 2 (Dapk2)

  • 货号：
    CSB-YP006500MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006500MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006500MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006500MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006500MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dapk2Death-associated protein kinase 2; DAP kinase 2; EC 2.7.11.1; DAP-kinase-related protein 1; DRP-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-370
  • 氨基酸序列
    MVQASMRSPN METFKQQKVE DFYDIGEELG SGQFAIVKKC REKSTGLEYA AKFIKKRQSR ASRRGVCREE IEREVSILRQ VLHPNIITLH DVYENRTDVV LILELVSGGE LFDFLAQKES LSEEEATSFI KQILDGVNYL HTKKIAHFDL KPENIMLLDK NIPIPHIKLI DFGLAHEIED GVEFKNIFGT PEFVAPEIVN YEPLGLEADM WSIGVITYIL LSGASPFLGD TKQETLANIT AVSYDFDEEF FSQTSELAKD FIRKLLVKET RKRLTIQEAL RHPWITPVDT QQAMVRRESV VNLENFKKQY VRRRWKLSFS IVSLCNHLTR SLMKKVHLRT SEDLRNCESD TEENIARRKA LHPRRRSSTS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Capable of regulating both type I apoptotic and type II autophagic cell death signals. The former involves caspase activation, chromatin and mitochondrial condensation while the latter involves caspase-independent cell death in conjunction with accumulation of mature autophagic vesicles, plasma membrane blebs, and nuclear condensation without DNA degradation. Mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation. Regulates granulocytes motility by controlling cell spreading and polarization.
  • 基因功能参考文献：
    1. Thyroid hormone promotes selective autophagy via induction of DAPK2-SQSTM1 cascade, which in turn protects hepatocytes from diethylnitrosamine-induced hepatotoxicity or carcinogenesis. PMID: 27653365
    2. This study links adipocyte expression of an autophagy-regulating kinase, lysosome-mediated clearance and fat cell lipid accumulation; it demonstrates obesity-related attenuated autophagy in adipocytes, and identifies DAPK2 dependence in this regulation. PMID: 26038578
    3. DAPK2 is strongly and specifically expressed in interstitial cells of the cortex, providing a useful marker for this important cell population PMID: 24906443
    4. The defect in chemotaxis in DAPK2-inactive granulocytes is likely a result of reduced polarization of the cells, mediated by a lack of MLC phosphorylation, resulting in radial F-actin and pseudopod formation. PMID: 24163421
    5. These results suggest that DAPK2 is one of the targets of cGK-I in apoptosis induction. PMID: 22580283
    6. The crystal and solution structures of murine DAPK2 were determined in the presence of the autoinhibitory domain, with and without bound nucleotides in the active site. Dimers of DAPK2 had a conformation that did not permit protein substrate binding. PMID: 21497605
    7. DAPK2 was substantially up-modulated during late erythropoiesis PMID: 18535204

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  • 亚细胞定位：
    Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, DAP kinase subfamily
  • 组织特异性：
    Expressed in peritubular interstitial cells of the renal cortex. Isoform 1 is found in the adult brain while isoform 2 is expressed in brains of embryos and young mice (at protein level).
  • 数据库链接：

    KEGG: mmu:13143

    STRING: 10090.ENSMUSP00000034944

    UniGene: Mm.304472