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  4. Recombinant Mouse Diacylglycerol kinase gamma (Dgkg)

Recombinant Mouse Diacylglycerol kinase gamma (Dgkg)

  • 货号：
    CSB-YP852784MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP852784MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP852784MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP852784MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP852784MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dgkg; Dagk3; Diacylglycerol kinase gamma; DAG kinase gamma; EC 2.7.1.107; 88 kDa diacylglycerol kinase; Diglyceride kinase gamma; DGK-gamma
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-788
  • 氨基酸序列
    MSEEQWVSLS SEEFDQLQKY SEYSSKKIKD VLAEFNEGGS LRQYDPHKPI SYDVFKLFMR AYLEVDLPQP LSTHLFLAFS QKPRQETPDH PKEGASSSEP NVSDYNSDNA AKADEACAPD TESKTTKTQA PSKELEAAAP WEDPGALASS SDAPVVYLKD VVCYLSLMET GRPQDKLEFM FRLYDSDENG LLDQAEMDQI VSQMLHVAQY LEWDPTELRP ILKEMLQGMD YDKDGFVSLQ EWINGGMTTI PLLVLLGMDD SGSKGDGRHA WTLKHFKKPT YCNFCRAMLM GVGKQGLCCI YCKYTVHQRC VSKTIHGCVK TNSKAKRSGE VMQHAWVEGN SSVKCDRCHK SIKCYQSVTA RHCVWCRMTF HRKCELSTVC DGGELKDHIL LPTSICPVSG DRQGGKSDGS VAAKGELVTQ YKIIPSPGTH PLLVLVNPKS GGRQGERILR KFHYLLNPEQ VFNLDNGGPT PGLNFFHDTP DFRVLACGGD GTVGWILDCI DKANFTKHPP VAVLPLGTGN DLARCLRWGG GYEGGSLTKI LKEIEQSPLV MLDRWYLEVM PREEVENGDQ VPYNIMNNYF SIGVDASIAH RFHMMREKHP EKFNSRMKNK LWYFEFGTSE TFAATCKKLH DHIELECDGV EVDLSNIFLE GIAILNIPSM YGGTNLWGET KKNRAVIRES RKSVTDPKEL KCCVQDLSDQ LLEVVGLEGA MEMGQIYTGL KSAGRRLAQC SSVTIRTNKL LPMQVDGEPW MQPQCTIKIT HKNQAPMMMG PPQKSSFFSL RRKSRSKD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Has no apparent specificity with regard to the acyl compositions of diacylglycerol. Specifically expressed in the cerebellum where it controls the level of diacylglycerol which in turn regulates the activity of protein kinase C gamma. Through protein kinase C gamma, indirectly regulates the dendritic development of Purkinje cells, cerebellar long term depression and ultimately cerebellar motor coordination.
  • 基因功能参考文献：
    1. A novel morphological function of the C-terminal DGK-gamma catalytic region in filopodia formation, independent of its enzymatic activity. PMID: 23054194
    2. DGKgamma functions through its catalytic action as an upstream suppressor of Rac1 and has a role in lamellipodium/ruffle formation PMID: 15102830
  • 亚细胞定位：
    Membrane. Cytoplasm, cytosol. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Eukaryotic diacylglycerol kinase family
  • 组织特异性：
    Expressed in hippocampus and cerebellar Purkinje cells and weakly and diffusely expressed in the granule cells.
  • 数据库链接：

    KEGG: mmu:110197

    STRING: 10090.ENSMUSP00000087371

    UniGene: Mm.194986