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  4. Recombinant Mouse Disintegrin and metalloproteinase domain-containing protein 10 (Adam10), partial

Recombinant Mouse Disintegrin and metalloproteinase domain-containing protein 10 (Adam10), partial

  • 中文名称：
    Recombinant Mouse Disintegrin and metalloproteinase domain-containing protein 10(Adam10),partial,Yeast
  • 货号：
    CSB-YP520309MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Disintegrin and metalloproteinase domain-containing protein 10(Adam10),partial,Yeast
  • 货号：
    CSB-EP520309MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Disintegrin and metalloproteinase domain-containing protein 10(Adam10),partial,Yeast
  • 货号：
    CSB-EP520309MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Disintegrin and metalloproteinase domain-containing protein 10(Adam10),partial,Yeast
  • 货号：
    CSB-BP520309MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Disintegrin and metalloproteinase domain-containing protein 10(Adam10),partial,Yeast
  • 货号：
    CSB-MP520309MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Adam10
  • Uniprot No.：
  • 别名：
    Adam10; Kuz; MadmDisintegrin and metalloproteinase domain-containing protein 10; ADAM 10; EC 3.4.24.81; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD antigen CD156c
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) at '687-Lys-|-Leu-688'. Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3. Mediates the proteolytic cleavage of IL6R and IL11RA, leading to the release of secreted forms of IL6R and IL11RA. Enhances the cleavage of CHL1 by BACE1. Cleaves NRCAM. Cleaves TREM2, resulting in shedding of the TREM2 ectodomain. Involved in the development and maturation of glomerular and coronary vasculature. During development of the cochlear organ of Corti, promotes pillar cell separation by forming a ternary complex with CADH1 and EPHA4 and cleaving CADH1 at adherens junctions. May regulate the EFNA5-EPHA3 signaling.
  • 基因功能参考文献：
    1. Data show that tetraspanin15 (Tspan15) is abundantly expressed in brain, where it specifically interacts with endogenous a disintegrin and metalloproteinase 10 (ADAM10). PMID: 29520422
    2. ADAM10(DC)(-/-) mice are resistant to IgE-mediated anaphylaxis. PMID: 28745029
    3. In the present study, we report that Nrxn3beta is processed by the metalloproteases ADAM10, ADAM17, and by the intramembrane-cleaving protease gamma-secretase, producing secreted neurexin3beta (sNrxn3beta) and a single intracellular domain (Nrxn3beta-ICD). Thea s show that sNrxn3beta produced by the cells of the dentate gyrus increases the spine density of newborn neurons. PMID: 27991559
    4. the synaptic localization of APP, ADAM10, and BACE1 in the mouse cerebral cortex, was examined. PMID: 26497029
    5. Pharmacological inhibition of ADAM10 reduces sEphrin-B2 levels in bronchoalveolar lavage and prevents lung fibrosis in mice. Consistent with the mouse data, ADAM10-sEphrin-B2 signaling is upregulated in fibroblasts from human subjects with idiopathic pulmonary fibrosis. PMID: 29058717
    6. Xenoestrogens biphenol-A and nonylphenol stimulate the release of EGFR-ligands by differentially activating ADAM17 or ADAM10. PMID: 28703301
    7. study confirms the importance of ICOSL shedding in ICOS/ICOSL function and expression and it identifies ADAM10 as the most important sheddase for controlling ICOSL levels PMID: 28814605
    8. Tspan3 is a central endocytic membrane component regulating the expression of ADAM10, presenilin and the amyloid precursor protein. PMID: 27818272
    9. these results show that ADAM10-Notch signaling in ovarian somatic cells governs the primordial follicle formation by controlling the development of ovarian pregranulosa cells. PMID: 27084580
    10. Findings provide evidence that ADAM10, and not ADAM17, is indispensable for proper retinal development as a regulator of NOTCH signaling. PMID: 27224017
    11. this study shows that during positive selection in the spleen, B-cell receptor signaling causes immature type 1 transitional B cells to become receptive to Notch ligands via Taok3-mediated surface expression of ADAM10 PMID: 28068307
    12. Thus, Leda-1/Pianp is constitutively processed by proprotein convertases, sheddases including MMPs and ADAM10/17 and intramembrane protease gamma-secretase. PMID: 27349870
    13. ADAM10 was dispensable for alpha-toxin-dependent xenophagic targeting of S. aureus, whereas a role for alpha-toxin attack on the plasma membrane was confirmed. PMID: 27147619
    14. ADAM10 was essentially involved in maxillofacial bone development. ADAM10 conditional knock-out KO mice present craniofacial dysmorphia and bone defects. Impaired osteoblast differentiation,proliferation and apoptosis underlie the bone deformity. PMID: 27221046
    15. Newborn mice deficient in ADAM10 exhibited organ-specific vascular defects. PMID: 27354212
    16. Findings demonstrate the direct requirement of ADAM10 in cortical radial migration and reveal the underlying mechanism by linking ADAM10-initiated regulated intramembrane proteolysis of Notch to the regulation of microtubule cytoskeleton through transcriptional control of Dcx expression PMID: 28158408
    17. our data afford the new insight that miR-103a inhibited abdominal aortic aneurysm (AAA) growth via targeting ADAM10, which might be a promising therapeutic strategy to alleviate AAA. PMID: 28357407
    18. ADAM10 gene was predominantly expressed in the neurons of the cerebral cortex, hippocampus, thalamus and cerebellar granular cells in adult mouse CNS. PMID: 27431484
    19. Matrix metalloproteases ADAM10 and TACE (ADAM17) cleave AXL receptor tyrosine kinase (Axl) in lupus-prone leukocytes. PMID: 27237127
    20. Loss of ADAM10 in murine liver results in hepatocyte necrosis and concomitant liver fibrosis. ADAM10 directly regulates expression of bile acid transporters but is dispensable for Notch2-dependent formation of the biliary system. Differentiation of liver progenitor cells to hepatocytes is augmented in the absence of ADAM10. PMID: 26942887
    21. Studies demonstrate a role for ADAM10 in the ectodomain shedding of LRP1 in the brain and the clearance of Abeta across the blood-brain barrier, which may provide a novel strategy for attenuating Abeta accumulation in the AD brain PMID: 27503326
    22. With a proteomic analysis of Adam10-deficient neurons the s identified 91, mostly novel ADAM10 substrate candidates, making ADAM10 a major protease for membrane proteins in the nervous system. PMID: 26802628
    23. Data suggest that large extracellular loop of Tspan14 mediates interaction with ADAM10, promotes ADAM10 maturation/trafficking to cell surface, and affects ADAM10 substrate specificity; ADAM10/Tspan14 interact in platelets/vascular endothelial cells. PMID: 26668317
    24. findings underscore the role of ADAM10 as an indispensable component of Notch signaling in SCs and for maintaining the SC pool PMID: 26453297
    25. established that endogenous IL-6R of both human and murine origin is shed by ADAM17 in an induced manner, whereas constitutive release of endogenous IL-6R is largely mediated by ADAM10 PMID: 26359498
    26. ADAM10 (and BACE1) are localized in synaptic vesicles in primary hippocampal neurons. PMID: 26296617
    27. B cell levels of ADAM10, specifically, are increased in allergic patients and Th2 prone WT mouse strains. PMID: 25933166
    28. This study demonstrated that a neuron-intrinsic function of ADAM10 in axonal regeneration. PMID: 26426268
    29. NG2 cleavage by ADAM10 yields an ectodomain present in the extracellular matrix and a C-terminal fragment that is subsequently further processed by the gamma-secretase to release an intracellular domain. PMID: 25387269
    30. These data suggest a critical role of Adam10 for leukocyte recruitment, inflammatory mediator production, and extracellular matrix degradation. PMID: 25659879
    31. Results indicate peroxisome proliferator-activated receptor alpha (PPARalpha) as an important factor regulating neuronal a disintegrin and metalloproteinase 10 ADAM10 expression and, thus, alpha-secretase proteolysis of amyloid precursor protein (APP). PMID: 26080426
    32. In mouse retinas, ADAM10 or gamma-secretase inhibition induced vascular sprouting and density in vivo, whereas attenuation of both ADAM10 and ADAM17 activity produced the opposite phenotype. PMID: 25218057
    33. Human and murine P2X7 activation induces the rapid shedding of CD23 from B cells, with a potential role for ADAM10 in this process. PMID: 25155463
    34. role of IFN-gamma, CXCL16, and ADAM10 in oxLDL-induced lipid accumulation in glomerular podocytes PMID: 24752304
    35. analysis of how ADAM9, 10, and 17 maturation requires processing at a newly identified Proprotein Convertase cleavage site PMID: 25795784
    36. Obvious loss of ADAM10 during prion infection in vitro and in vivo highlights that ADAM10 may play essential pathophysiological roles in prion replication and accumulation. PMID: 24771043
    37. cytoplasmic domain of ADAM10 negatively influences constitutive shedding through an ER retention motif, whereas the cytoplasmic domain and prodomain processing are not required for the rapid activation of ADAM10-dependent shedding events. PMID: 25605720
    38. loss of expression exacerbates Staphylococcus aureus skin infection, yet affords protection against lethal pneumonia PMID: 24820433
    39. TACI is sequentially processed by a disintegrin and metalloproteinase 10 and gamma-secretase. PMID: 25505277
    40. the functions of Adam10 in determining the fate of collecting duct cells are more complex than those of a simple upstream factor in a linear pathway involving Notch and Foxi1 PMID: 24904084
    41. ADAM10 plays an essential role in the activation of Notch-1 signaling and has a remarkable effect on neuronal maintenance in adult mouse brain PMID: 25445276
    42. ADAM10 acts in a cell autonomous manner within the intestinal crypt compartment to regulate Notch signaling and progenitor cell lineage specification and crypt-based columnar cell maintenance. PMID: 25038433
    43. Collectively these data show that the ADAM10 protease is an important factor in mast cell migration and tissue distribution, and can be manipulated by environmental cues. PMID: 24950026
    44. Entorhinal denervation caused a transient upregulation of Adam10 mRNA and Adam17 mRNA in the denervated outer molecular layer of the dentate gyrus. PMID: 24404197
    45. Thus, with regard to leukocyte migration, leukocyte-expressed ADAM10 but not ADAM17 displays proinflammatory activities and may therefore serve as a target to limit inflammatory cell recruitment. PMID: 24833351
    46. These studies further suggest that inhibiting ADAM 10 activity could be of therapeutic benefit in AKI. PMID: 24662289
    47. these findings indicate that a tightly controlled ADAM10 expression is needed to balance hematopoietic cell-fate decisions in adult mice PMID: 24239882
    48. Estrogen receptor alpha mediated signaling activates ADAM10 to promote amyloid precursor protein processing. PMID: 23977176
    49. findings presented here suggest that ADAM10 in endothelial cells is required for the proper development and function of the specialized vasculature in the bone during the process of endochondral ossification. PMID: 24108673
    50. TIM-1 and TIM-4 are novel targets for ADAM10- and ADAM17-mediated ectodomain shedding. PMID: 24286866

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, clathrin-coated vesicle. Cell projection, axon. Cell projection, dendrite. Cell junction, adherens junction. Cytoplasm.
  • 组织特异性：
    Expressed in the brain, specifically in neurons and astrocytes (at protein level). Expressed in inner and outer pillar cells of the organ of Corti (at protein level). Expressed in kidney and lung.
  • 数据库链接：

    KEGG: mmu:11487

    STRING: 10090.ENSMUSP00000063839

    UniGene: Mm.3037