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  4. Recombinant Mouse Double C2-like domain-containing protein beta (Doc2b)

Recombinant Mouse Double C2-like domain-containing protein beta (Doc2b)

  • 货号：
    CSB-YP007092MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007092MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007092MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007092MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007092MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Doc2b
  • Uniprot No.：
  • 别名：
    Doc2bDouble C2-like domain-containing protein beta; Doc2-beta
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-412
  • 氨基酸序列
    MTLRRRGEKA TISIQEHMAI DVCPGPIRPI KQISDYFPRF PRGLPPTAAP RAPAPPDAPA RSPAASASPR SPSDGARDDD EDVDQLFGAY GASPGPSPGP SPARPPAKPP EDEPDVDGYE SDDCTALGTL DFSLLYDQEN NALHCTISKA KGLKPMDHNG LADPYVKLHL LPGASKANKL RTKTLRNTLN PSWNETLTYY GITDEDMVRK TLRISVCDED KFRHNEFIGE TRVPLKKLKP NHTKTFSICL EKQLPVDKAE DKSLEERGRI LISLKYSSQK QGLLVGIVRC AHLAAMDANG YSDPYVKTYL KPDVDKKSKH KTAVKKKTLN PEFNEEFCYE IKHGDLAKKT LEVTVWDYDI GKSNDFIGGV VLGINAKGER LKHWFDCLKN KDKRIERWHT LTNELPGAVL SD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes.
  • 基因功能参考文献：
    1. Reduction of DOC2B is an early feature of T1 diabetes (T1D), and DOC2B abundance may serve as a valuable in vivo indicator of beta-cell mass and an early biomarker of T1D. PMID: 29506054
    2. Doc2b enrichment in the beta-cell protects against diabetogenic and proapoptotic stress. PMID: 29661782
    3. Mutating Ca(2+)-coordinating aspartates in the C2A-domain localizes Doc2B permanently at the plasma membrane, and renders an upstream priming step Ca(2+)-independent, whereas a separate function in downstream priming depends on SNARE-binding, Ca(2+)-binding to the C2B-domain of Doc2B, interaction with ubMunc13-2 and the presence of synaptotagmin-1. PMID: 29274147
    4. Data demonstrate that Munc13-1 and DOC2B have different effects on network activity and that by enhancing asynchronous release, DOC2B exerts its properties to increase spiking activity and elevate synchronization between neurons within network bursts PMID: 23537531
    5. Hence these data support a working model wherein Doc2b functions as a docking platform/scaffold for transient interactions with the multiple Munc18 isoforms operative in insulin release, promoting SNARE assembly. PMID: 25190515
    6. Doc2b is a limiting factor in SNARE exocytosis events pertinent to glycaemic regulation in vivo. Doc2b enrichment may provide a novel means to simultaneously boost islet and skeletal muscle function in vivo in the treatment and/or prevention of diabetes. PMID: 24705606
    7. Mutations that disrupt Ca(2)-binding activity endow Doc2beta with novel functional properties during synaptic transmission. PMID: 24356452
    8. Doc2b acts to inhibit vesicle priming during prolonged calcium elevations, thus protecting unprimed vesicles from fusing prematurely, and redirecting them to refill the readily releasable pool after relaxation of the calcium signal. PMID: 24133251
    9. Doc2b is a key positive regulator of Munc18c-syntaxin 4-mediated insulin secretion as well as of insulin responsiveness in skeletal muscle, and thus a key effector for glucose homeostasis in vivo. PMID: 22698913
    10. The data of this study suggested that Doc2s are modulators of spontaneous synaptic transmission that act by a Ca(2+)-independent mechanism. PMID: 21521611
    11. study found that Doc2b is a high-affinity Ca2+ sensor for half (hippocampus) or most (cerebellum) of the spontaneous neurotransmitter release events in CNS synapses PMID: 20150444
    12. tyrosine phosphorylation of Munc18c induces a switch in binding specificity from syntaxin 4 to Doc2beta PMID: 18541526
    13. We conclude that DOC2B is a calcium-dependent priming factor and its activity at the PM enables efficient expansion of the fusion pore, leading to increased catecholamine release. PMID: 18596155
    14. Fndings indicate that DOC2b is a positive SNARE regulator for GLUT4 vesicle fusion and mediates insulin-stimulated glucose transport in adipocytes. PMID: 19033398
    15. These data suggest that DOC2b may be a regulator for delayed (second-phase) insulin secretion in MIN6 cells. PMID: 19410553

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  • 亚细胞定位：
    Cytoplasm. Cytoplasmic granule. Cell membrane; Peripheral membrane protein. Note=Translocates to the plasma membrane in a calcium-dependent manner.
  • 组织特异性：
    Widely expressed. Expressed in pancreatic islet cells (at protein level).
  • 数据库链接：

    KEGG: mmu:13447

    STRING: 10090.ENSMUSP00000021209

    UniGene: Mm.5137