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  4. Recombinant Mouse Drebrin (Dbn1)

Recombinant Mouse Drebrin (Dbn1)

  • 货号：
    CSB-YP889558MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP889558MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP889558MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP889558MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP889558MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Dbn1
  • Uniprot No.：
  • 别名：
    Dbn1; DrbaDrebrin; Developmentally-regulated brain protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-706
  • 氨基酸序列
    AGVSFSGHR LELLAAYEEV IREESAADWA LYTYEDGSDD LKLAASGEGG LQELSGHFEN QKVMYGFCSV KDSQAALPKY VLINWVGEDV PDARKCACAS HVAKVAEFFQ GVDVIVNASS VEDIDAGAIG QRLSNGLARL SSPVLHRLRL REDENAEPVG TTYQKTDAAV EMKRINREQF WEQAKKEEEL RKEEERKKAL DARLRFEQER MEQERQEQEE RERRYREREQ QIEEHRRKQQ SLEAEEAKRR LKEQSIFGDQ RDEEEESQMK KSESEVEEAA AIIAQRPDNP REFFRQQERV ASASGGSCDA PAPAPFNHRP GRPYCPFIKA SDSGPSSSSS SSSSPPRTPF PYITCHRTPN LSSSLPCSHL DSHRRMAPTP IPTRSPSDSS TASTPIAEQI ERALDEVTSS QPPPPPPPPP PTQEAQETTP SLDEELSKEA KVTAAPEVWA GCAAEPPQAQ EPPLLQSSPL EDSMCTESPE QAALAAPAEP AASVTSVADV HAADTIETTT ATTDTTIANN VTPAAASLID LWPGNGEEAS TLQAEPRVPT PPSGAEASLA EVPLLNEAAQ EPLPPVGEGC ANLLNFDELP EPPATFCDPE EEVGETLAAS QVLTMPSALE EVDQVLEQEL EPEPHLLTNG ETTQKEGTQA SEGYFSQSQE EEFAQSEEPC AKVPPPVFYN KPPEIDITCW DADPVPEEEE GFEGGD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Actin cytoskeleton-organizing protein that plays a role in the formation of cell projections. Required for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS. Plays a role in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to the dendritic spines. Involved in memory-related synaptic plasticity in the hippocampus.
  • 基因功能参考文献：
    1. Functional perturbations of drebrin demonstrate that proximal leading process microtubule-actomyosin coupling steers the direction of centrosome and somal migration, as well as the switch from tangential to radial migration. PMID: 28230156
    2. Hippocampal Drebrin protein expression levels in adult APP/PS1 mice decline with age. Behavioral data demonstrated that cognitive and probe activities decline with age. A correlation analysis showed that the expression level of the Drebrin protein significantly correlatives with behavioral performance in Alzheimer's disease mice. PMID: 28597477
    3. In subventricular zone of Dbn1-null mice number of neuroblasts and cell proliferation decreased, although cell death remained unchanged. PMID: 28833685
    4. Inhibition of cortactin or dynamin-2 abrogated the increased virus entry observed in DBN1-deficient cells, suggesting that DBN1 suppresses dynamin-mediated endocytosis via interaction with cortactin. PMID: 28416666
    5. Drebrin A is highly enriched in dendritic spines, but its effects on actin morphology, dynamics, and interplay with other actin regulators are yet to be clarified. Here we review recent advances in understanding drebrin effects on actin morphology and dynamics. PMID: 28865015
    6. Drebrin A can be found co-clustering with NR2B-containing NMDARs at the plasma membrane, while NR2A-containing NMDARs co-traffic into the spine cytoplasm but do not co-cluster at the plasma membrane. PMID: 28865018
    7. these findings suggest that during cell migration drebrin is involved in retraction processes but not in lamellipodia formation. The novel, sizable juxtanuclear drebrin-enriched zone remains to be characterized in detail with respect to its molecular assembly and functions. PMID: 28865029
    8. Study determines the specific roles of drebrin in the regulation of the axonal cytoskeleton, and provides evidence that drebrin contributes to the coordination of the actin and microtubule cytoskeleton during the initial stages of axon branching. PMID: 26731339
    9. Drebrin reduces SMC activation through its interaction with the actin cytoskeleton but independently of its interaction with Homer scaffolds to prevent neointima formation. PMID: 27013612
    10. Data suggest further studies to elucidate the effect of drebrin (Dbn) on the development and progression of Alzheimer's disease (AD). PMID: 28398234
    11. Study demonstrated that isoform conversion of drebrin is critical, and that drebrin A is indispensable for adult hippocampal synaptic plasticity and hippocampus-dependent fear learning; the phenotype of drebrin A knockout mice suggests functional differences between drebrin A and drebrin E PMID: 26970584
    12. These findings provide unprecedented experimental support for a role of drebrin in the regulation of memory-related synaptic plasticity and neurotransmitter receptor signaling PMID: 25865831
    13. Dbn1 regulates systemic anaphylaxis and IgE/Fcgr1-induced degranulation in mast cells by regulating actin reorganization and actin dynamics. PMID: 26056254
    14. SAHA likely inhibits ADDL-induced drebrin loss from dendritic spines by stabilizing drebrin in these structures, rather than by increasing drebrin clusters or dendritic protrusions PMID: 25058791
    15. These results suggest that Cdk5-p35 regulates neuronal migration through phosphorylation of drebrin in growth cone processes. PMID: 24637538
    16. Double knockout Psen1Psen2 leads to a decrease in immunoreactivity for drebrin A at both synaptic and nonsynaptic areas of CA1 hippocampus. PMID: 22715045
    17. The density of drebrin-positive glutamatergic synapses formed on GABAergic neurons is lower than those on glutamatergic neurons. PMID: 20600648
    18. These results suggest that the actin-binding domain of drebrin is centered on actin subdomain 2 and may adopt a folded conformation upon binding to F-actin. PMID: 20347847
    19. Drebrin A plays an indispensable role in some processes of generating fear learning and memory. PMID: 19837137
    20. Drebrin is involved in regulation of actin dynamics also outside the nervous system. Drebrin is one of the actin-binding proteins regulating the actomyosin system and the dynamics of mesangial cells and foot processes in podocytes. PMID: 12761245
    21. These data confirm the role of drebrin E2 in the formation of branching processes and further indicate that during cell migration, the protein contributes to retraction of the cell body and the tail but not to lamellipodia formation. PMID: 16780834
    22. The drebrin 1 located in the dendritic Spine of adult mouse cerebral cortex and its content correleates with spin head size. PMID: 17559090
    23. These findings indicate that drebrin A is required for the rapid (<30 minutes) form of homeostatic synaptic plasticity at excitatory synapses of adult cortices, whereas drebrin E is sufficient for maintaining basal NR2A levels within spines. PMID: 19711416

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  • 亚细胞定位：
    Cytoplasm. Cell projection, dendrite. Cytoplasm, cell cortex. Cell junction. Cell projection. Cell projection, growth cone.
  • 组织特异性：
    Expressed in the hippocampus, with expression in the pyramidal cells of CA1, CA2 and CA3 and in the granule cells of the dentate gyrus (at protein level). Highly expressed in brain, also present in stomach and to a lesser degree in kidney, colon, and urin
  • 数据库链接：

    KEGG: mmu:56320

    STRING: 10090.ENSMUSP00000021950

    UniGene: Mm.19016