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  4. Recombinant Mouse Dual specificity mitogen-activated protein kinase kinase 5 (Map2k5)

Recombinant Mouse Dual specificity mitogen-activated protein kinase kinase 5 (Map2k5)

  • 货号：
    CSB-YP893011MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP893011MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP893011MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP893011MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP893011MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Map2k5; Mek5; Mkk5; Prkmk5; Dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAPKK 5; EC 2.7.12.2; MAPK/ERK kinase 5; MEK 5
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-448
  • 氨基酸序列
    MLWLALGPFC AMENQVLVIR IKIPNSGAVD WTVHSGPQLL FRDVLDVIGQ VLPEATTTAF EYEDEDGDRI TVRSDEEMKA MLSYYYSTVM EQQVNGQLIE PLQIFPRACK PPGERNIHGL KVNTRAGPSQ HTSPVVSDSL PSNSLKKSSA ELRKILANGQ MNEQDIRYRD TLGHGNGGTV YKAHHVPSGK ILAVKVILLD ITLELQKQIM SELEILYKCD SSYIIGFYGA FFVENRISIC TEFMDGGSLD VYRKIPEHVL GRIAVAVVKG LTYLWSLKIL HRDVKPSNML VNTGGQVKLC DFGVSTQLVN SIAKTYVGTN AYMAPERISG EQYGIHSDVW SLGISFMELA LGRFPYPQIQ KNQGSLMPLQ LLQCIVDEDS PVLPLGEFSE PFVHFITQCM RKQPKERPAP EELMGHPFIV QFNDGNSTVV SMWVCRALEE RRSQQGPP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.
  • 基因功能参考文献：
    1. MEK5 overexpression inhibits differentiation of osteoblasts. PMID: 25998381
    2. the MEK5-ERK5 MAP kinase signaling pathway has a critical role in BAFF-induced mature B cell survival and homeostatic maintenance of B2 cell numbers PMID: 25987726
    3. The MEK5/ERK5 pathway also mediates FSS-induced Runx-2 expression in osteoblast differentiation. PMID: 24111522
    4. finding suggested that REST promoted primordial germ cells survival via regulation of the Mek5 expression PMID: 23022299
    5. PB1 domain mediates the association of MEKK2 and MEKK3 with MEK5 and that the respective PB1 domains of these kinases are critical for regulation of the ERK5 pathway. PMID: 12912994
    6. MEK5 is an activator of ERK5 and an essential regulator of cell survival that is required for normal embryonic development PMID: 15601854
    7. the N terminus of MEK5alpha is critical for the specific organization of the components of the ERK5 signaling pathway PMID: 16260599
    8. MEK5 and big MAP kinase 1 (BMK1) function downstream of MEKK3 in a signaling cascade that induces calcineurin activity through phosphorylation of MCIP1 PMID: 16415348
    9. A noncanonical function of Map2k5 domain is decribed for coordinated erk5 kinase and map2k7 signaling. PMID: 17452462
    10. Results show the critical involvement of the MEK5-ERK5 pathway in thymocyte development distinct from that of ERK1/2 and highlight the importance of the MAPK network in mediating differential effects pertaining to T-cell differentiation and apoptosis. PMID: 18548009
    11. Transfection of myoblasts with dominant-negative MEK5 blocked the pro-myogenic action of IGF-2. PMID: 19654213

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
  • 数据库链接：

    KEGG: mmu:23938

    STRING: 10090.ENSMUSP00000034920

    UniGene: Mm.325746