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  4. Recombinant Mouse Dual specificity tyrosine-phosphorylation-regulated kinase 1A (Dyrk1a)

Recombinant Mouse Dual specificity tyrosine-phosphorylation-regulated kinase 1A (Dyrk1a)

  • 中文名称：
    小鼠Dyrk1a重组蛋白
  • 货号：
    CSB-YP736810MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Dyrk1a重组蛋白
  • 货号：
    CSB-EP736810MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Dyrk1a重组蛋白
  • 货号：
    CSB-EP736810MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Dyrk1a重组蛋白
  • 货号：
    CSB-BP736810MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Dyrk1a重组蛋白
  • 货号：
    CSB-MP736810MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dyrk1a; DyrkDual specificity tyrosine-phosphorylation-regulated kinase 1A; EC 2.7.12.1; Dual specificity YAK1-related kinase; MP86; Protein kinase minibrain homolog; MNBH
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-763
  • 氨基酸序列
    MHTGGETSAC KPSSVRLAPS FSFHAAGLQM AAQMPHSHQY SDRRQPSISD QQVSALPYSD QIQQPLTNQV MPDIVMLQRR MPQTFRDPAT APLRKLSVDL IKTYKHINEV YYAKKKRRHQ QGQGDDSSHK KERKVYNDGY DDDNYDYIVK NGEKWMDRYE IDSLIGKGSF GQVVKAYDRV EQEWVAIKII KNKKAFLNQA QIEVRLLELM NKHDTEMKYY IVHLKRHFMF RNHLCLVFEM LSYNLYDLLR NTNFRGVSLN LTRKFAQQMC TALLFLATPE LSIIHCDLKP ENILLCNPKR SAIKIVDFGS SCQLGQRIYQ YIQSRFYRSP EVLLGMPYDL AIDMWSLGCI LVEMHTGEPL FSGANEVDQM NKIVEVLGIP PAHILDQAPK ARKFFEKLPD GTWSLKKTKD GKREYKPPGT RKLHNILGVE TGGPGGRRAG ESGHTVADYL KFKDLILRML DYDPKTRIQP YYALQHSFFK KTADEGTNTS NSVSTSPAME QSQSSGTTSS TSSSSGGSSG TSNSGRARSD PTHQHRHSGG HFAAAVQAMD CETHSPQVRQ QFPAPLGWSG TEAPTQVTVE THPVQETTFH VAPQQNALHH HHGNSSHHHH HHHHHHHHHG QQALGNRTRP RVYNSPTNSS STQDSMEVGH SHHSMTSLSS STTSSSTSSS STGNQGNQAY QNRPVAANTL DFGQNGAMDV NLTVYSNPRQ ETGIAGHPTY QFSANTGPAH YMTEGHLAMR QGADREESPM TGVCVQQSPV ASS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6. Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis.
  • 基因功能参考文献：
    1. Chronic Dyrk1 inhibition reversed cognitive deficits in Alzheimer's disease transgenic mice via reduction of APP and phosphorylated tau pathology. PMID: 28779511
    2. Normalizing Dyrk1A gene dosage in Ts65Dn mouse rescued the density of senescent cells in cingulate cortex, hippocampus and septum, prevented cholinergic neuron degeneration, and reduced APP expression in hippocampus, Abeta load in cortex and hippocampus, expression of phosphorylated tau at the Ser202 residue in hippocampus and cerebellum, and levels of total tau in the cortex, hippocampus and cerebellum. PMID: 28647555
    3. Depleting Huntingtin-associated protein 1 (Hap1) promoted the dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A)-DDB1 and CUL4 associated factor 7 (Dcaf7) interaction and increased the DYRK1A protein level. PMID: 28137862
    4. Down-regulation of Dyrk1A returned returned phosphatidylcholine in trisomic cells to original levels. PMID: 26803494
    5. Taken together, these results strongly suggested that DYRK1A bound to CDKL5 and phosphorylated it on Ser-308, thus interfering with its nuclear localization. PMID: 27840050
    6. Identify Dyrk1a as a novel negative regulator of D-cyclin-mediated Rb1/E2f-signalling. As dysregulation of this pathway with impaired cardiomyocyte proliferation leads to cardiomyopathy. PMID: 27056896
    7. study identifies DYRK1A-STAT as a signaling pathway responsible for the differentiation of neural progenitors into astrocytes, with direct implication for the anomalies in brain development observed in Down syndrome PMID: 26373433
    8. This study identified decreased neuronal firing rate and deficits in gamma frequency in the prefrontal cortices of transgenic mice overexpressing Dyrk1A. PMID: 27030752
    9. Increased Dyrk1a gene dosage is a major contributing factor to the abnormal appendicular skeletal phenotype observed in adolescent Ts65Dn Down syndrome mice. PMID: 26206885
    10. the increased immunostaining of DYRK1A in HIV+ brains without pathology points at dysregulation of DYRK1A as an early event in the neuronal complications of HIV infection. PMID: 26534959
    11. Our results identify DYRK1A as a physiologically relevant regulator of Treg cell differentiation and suggest a broader role for other DYRK family members in immune homeostasis. PMID: 25998054
    12. these data suggest that the dosage imbalance of genes other than Dyrk1a is involved in the development of the prenatal bone phenotype in Ts65Dn embryos. PMID: 25556111
    13. Show the crucial role of the DYRK1A pathway in the regulation of beta cell mass and carbohydrate metabolism in vivo. Activating the DYRK1A pathway could thus represent an innovative way to increase functional beta cell mass. PMID: 24870561
    14. Dyrk1A has a role in phosphorylation and inactivation of GSK3beta PMID: 25477508
    15. Study shows that DYRK1A overexpression affects pathways involved in synaptogenesis and synaptic plasticity and influences excitation/inhibition balance toward inhibition PMID: 24801365
    16. These data provide new insight into the mechanisms that control cell death in brain DA neurons and they show that deregulation of developmental apoptosis may contribute to the phenotype of patients with imbalanced DYRK1A gene dosage. PMID: 24922073
    17. DYRK1A is a critical kinase for beta cell growth as Dyrk1a-haploinsufficient mice show a diabetic profile PMID: 24477974
    18. This study supports the hypothesis that intracellular distribution and compartment-specific functions of DYRK1A may depend on its phosphorylation pattern. PMID: 24327345
    19. DYRK1A overexpression decreases plasma lecithin:cholesterol acyltransferase activity and apolipoprotein A-I levels. PMID: 23920041
    20. Mice bearing only one additional copy of Dyrk1a have the same retinal phenotype as Ts65Dn mice. PMID: 23512985
    21. These data shed light on previously undisclosed participation of DYRK1A in adult prefrontal cortex dendritic structures and synaptic plasticity PMID: 24453307
    22. dosage of DYRK1A and DSCR1 is critical for proper neurogenesis through NFATc and provide a potential mechanism to explain the neurodevelopmental defects in Down's syndrome PMID: 24352425
    23. these results show Dyrk1A as a critical player in the pathophysiology of Down syndrome and define Dyrk1A as a therapeutic target in adult trisomic mice. PMID: 23220201
    24. 55 of 111 in: PLoS One. 2013;8(1) (dup#6) Dyrk1A is dynamically expressed on subsets of motor neurons and in the neuromuscular junction: possible role in Down syndrome PMID: 23342120
    25. Hepatocyte-specific Dyrk1a gene transfer rescues plasma apolipoprotein A-I levels and aortic Akt/GSK3 pathways in hyperhomocysteinemic mice. PMID: 23429073
    26. These observations demonstrate ERK and Akt activation induced by Dyrk1A in the brain of hyperhomocysteinemic mice. PMID: 22923366
    27. Engineered changes in Dyrk1A gene dosage in vivo strongly alter the postnatal dendritic arborization processes with a similar progression than in humans PMID: 22215728
    28. Dyrk1a has two roles in the development process: shaping the brain and controlling the structure of neuronal components. PMID: 22293606
    29. mice that overexpress DYRK1A have an increased NQO1 expression and activity through an NRF2 mechanism, independent of arylhydrocarbon receptor; results reveal a link between DYRK1A and NRF2 signaling pathway PMID: 22178546
    30. Dyrk1a has a role in megakaryoblastic leukemia in Down syndrome and the murine model PMID: 22354171
    31. Our data show a distinctive expression pattern of DYRK1A forms nonphosphorylated at Tyr-145 and Tyr-147 in the brain tissue PMID: 21878370
    32. a direct link between Dyrk1A and RCAN1 in the Caln-NFAT signaling and Tau hyperphosphorylation pathways PMID: 21965663
    33. DYRKIA regulates pre-mRNA splicing in spermatogonia and proliferation of spermatogonia and Sertoli cells by phosphorylating the spliceosomal component SAP155 PMID: 21553260
    34. myocardial expression of DYRK1A failed to reduce hypertrophy PMID: 21273244
    35. the regulation of DYRK1A by REST in a negative feedback loop suggests that DYRK1A and REST are closely related in neurodevelopment. PMID: 21252229
    36. Data reveal an important role for DYRK1A in the regulation of DREAM activity and entry into quiescence. PMID: 21498570
    37. Down syndrome-like brain splicing machinery malfunctions in Dyrk1A-overexpressing transgenic mice. PMID: 20600907
    38. Data show that both SPRED1 and SPRED2 inhibit the ability of DYRK1A to phosphorylate its substrates. PMID: 20736167
    39. Study shows that miR-199b is a direct calcineurin/NFAT target gene that increases in expression in mouse and human heart failure, and targets the nuclear NFAT kinase dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a) PMID: 21102440
    40. Dyrk1A is part of a molecular pathway involved in the regulation of biased epidermal growth factor receptor signaling in the progeny of dividing neural stem cells of the adult subependymal zone PMID: 20804972
    41. up-regulation of Dyrk1A contributes to altered neuronal proliferation in DS through specific phosphorylation of p53 at Ser-15 and subsequent p21(CIP1) induction. PMID: 20696760
    42. Mice heterozygous for Dyrk1A exhibit decreased brain size, motor abnormalities and cognitive deficits in the adult. PMID: 19660545
    43. DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. PMID: 20167603
    44. DYRK1A is a novel determinant of the methionine-homocysteine cycle in mouse models PMID: 19844572
    45. Data show that forced expression of NFATc1 induced DYRK1A expression, suggesting a negative feedback loop. PMID: 19801542
    46. Gli1 transcriptional activity may be subjected to further regulation in the cell nucleus by a pathway distinct from Shh signaling, one mediated by Dyrk1 PMID: 12138125
    47. Role of Dyrk1A in brain abnormalities and developmental delay PMID: 12192061
    48. Dyrk1A is distributed in widespread areas of the adult CNS and in different subcellular compartments. Considering also previous reports demonstrating the involvement of Dyrk1A in developmental events, it is suggested that Dyrk1A has several functions. PMID: 12576186
    49. Transgenic mice present DYRK1A overexpression in brain motor areas along with specific motor dysfunction, including impairment of motor learning and alteration of the organization of locomotor behavior. PMID: 14751778
    50. DYRK1A overexpressing 152F7 mice display learning impairment and hyperactivity during development. Additionally, 152F7 mice display increased brain weight and neuronal size. PMID: 15198122

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  • 亚细胞定位：
    Nucleus speckle.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MNB/DYRK subfamily
  • 组织特异性：
    Detected in brain (at protein level). Expressed in a variety of embryonic and adult tissues. Expressed abundantly in neurons of the brain, spinal cord, and retina in developing embryos.
  • 数据库链接：

    KEGG: mmu:13548

    STRING: 10090.ENSMUSP00000023614

    UniGene: Mm.310973