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  4. Recombinant Mouse E3 SUMO-protein ligase PIAS1 (Pias1), partial

Recombinant Mouse E3 SUMO-protein ligase PIAS1 (Pias1), partial

  • 货号：
    CSB-YP017957MO1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017957MO1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017957MO1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017957MO1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017957MO1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pias1; Ddxbp1; E3 SUMO-protein ligase PIAS1; EC 2.3.2.27; DEAD/H box-binding protein 1; Protein inhibitor of activated STAT protein 1; RING-type E3 ubiquitin transferase PIAS1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 表达区域：
    1-400
  • 氨基酸序列
    MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPPTLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB.
  • 基因功能参考文献：
    1. these results indicate that PIAS1 is a positive regulator of MYC. PMID: 27239040
    2. PIAS1 overexpression exacerbated mutant Huntingtin-associated phenotypes and aberrant protein accumulation PMID: 27146268
    3. Pias1 expression in primary myoblasts enhances the induction of cardiac muscle genes MyoD, Myogenin and Myomaker. Endothelial cell specific inactivation of Pias1 in vivo impairs yolk sac erythrogenesis, angiogenesis and recapitulates loss of myocardium muscle mass. Pias1 is an essential gene for YS erythropoiesis and vasculogenesis in vivo. PMID: 27155222
    4. Data suggest overexpression of Pias1 in white adipose tissue (WAT) of obesity/prediabetes improves insulin resistance; knockdown of Pias1 in normal WAT leads to insulin resistance; Pias1 expression in WAT is down-regulated by c-Jun N-terminal kinase. PMID: 26324179
    5. A novel role of PIAS1 in maintaining the quiescence of dormant hematopoietic stem cells and in the epigenetic repression of the myeloerythroid program. PMID: 24357619
    6. The present study showed that PIAS1 functions as a SUMO E3 ligase of C/EBPbeta to regulate adipogenesis. PMID: 24061474
    7. Piasy represses the synergistic activation of PITX2 with interacting co-factors and Piasy represses Pias1 activation of PITX2 transcriptional activity. PMID: 23515314
    8. MAPK-activated protein kinase-2 limits endothelial inflammation via the PIAS1 S522 phosphorylation-mediated increase in PIAS1 transrepression and SUMO ligase activity. PMID: 23202365
    9. PIAS1 modifies covalent modification of Blimp-1 by SUMO-1 at lysine 816. PMID: 22555612
    10. PIAS1 negatively regulates ubiquitination of Msx1 homeoprotein independent of its SUMO ligase activity. PMID: 21717107
    11. PIAS1 restricts the differentiation of natural T(reg) cells by maintaining a repressive chromatin state of the Foxp3 promoter. PMID: 20966256
    12. Pias1-dependent SUMOylation influences Gli protein activity PMID: 20711444
    13. Protein inhibitors of activated STAT resemble scaffold attachment factors and function as interacting nuclear receptor coregulators. PMID: 11877418
    14. PIAS1 has a role in sumoylation of focal adhesion kinase and activates its autophosphorylation PMID: 14500712
    15. PIAS1 is a novel negative regulator of NF-kappaB. PMID: 15657437
    16. PIAS1 is required for the appropriate localization and retention of Msx1 at the nuclear periphery in myoblast cells. PMID: 16600910
    17. We show that PIAS1, which functions as a small ubiquitin-like modifier (SUMO) E3 ligase, associates with PTP1B in mammalian fibroblasts and catalyses sumoylation of PTP1B. PMID: 17159996
    18. The results identify a signaling pathway in which proinflammatory stimuli activate the IKKalpha-mediated sumoylation-dependent phosphorylation of PIAS1 for the immediate repression of inflammatory gene activation. PMID: 17540171
    19. These data suggest that AIRE and PIAS1 interact functionally to regulate the activities of the target genes of AIRE. PMID: 18083234
    20. PIAS1 promotes TGFbeta-induced activation of SM alpha-actin gene expression at least in part by promoting sumoylation and degradation of the TCE repressor protein, KLF4. PMID: 18927467
    21. Data from microarray analysis supports the conclusion that PIAS1 specfically regulates a subset of interferon-induced genes PMID: 15311277

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  • 亚细胞定位：
    Nucleus speckle. Nucleus, PML body.
  • 蛋白家族：
    PIAS family
  • 组织特异性：
    Expressed in kidney, heart, spleen, brain and cerebellum; weak expression, if any, in liver and lung.
  • 数据库链接：

    KEGG: mmu:56469

    STRING: 10090.ENSMUSP00000096248

    UniGene: Mm.431253