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  4. Recombinant Mouse E3 SUMO-protein ligase PIAS4 (Pias4)

Recombinant Mouse E3 SUMO-protein ligase PIAS4 (Pias4)

  • 货号：
    CSB-YP878109MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP878109MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP878109MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP878109MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP878109MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pias4; Piasg; E3 SUMO-protein ligase PIAS4; EC 2.3.2.27; PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; PIAS-gamma; RING-type E3 ubiquitin transferase PIAS4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-507
  • 氨基酸序列
    AAELVEAKN MVMSFRVSDL QMLLGFVGRS KSGLKHELVT RALQLVQFDC SPELFKKIKE LYETRYAKKS AEPGPQAPRP LDPLALHSMP RTPLSGPTVD YPVLYGKYLN GLGRLPTKTL KPEVRLVKLP FFNMLDELLK PTELVPQSAE KLQESPCIFA LTPRQVEMIR NSRELQPGVK AVQVVLRICY SDTSCPQEDQ YPPNIAVKVN HSYCSVPGYY PSNKPGVEPK RPCRPINLTH LMYLSSATNR ITVTWGNYGK SYSVALYLVR QLTSSDLLQR LKTIGVKHPE LCKALVKEKL RLDPDSEIAT TGVRVSLICP LVKMRLSVPC RAETCAHLQC FDAVFYLQMN EKKPTWMCPV CDKPAAYDQL IIDGLLSKIL SECEGADEIE FLAEGSWRPI RAEKEPSCSP QGPILVLGTS DANGLAPASS TPGIGSGLSG PGSAGSGAGA AGSLENGKTG ADVVDLTLDS SSSSEDEDED EDDDEDEDEG PRPKRRCPFQ KGLVPAC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.
  • 基因功能参考文献：
    1. study demonstrates that Piasy may prevent exaggerated transcription of IFNI by Rbp2-mediated demethylation of H3K4me3 of IFNI, avoiding excessive immune responses PMID: 28970247
    2. PIAS4 mediated SIRT1 repression led to SMAD3 hyperacetylation and enhanced SMAD3 binding to fibrogenic gene promoters and PIAS4 promoted hepatic stellate cells activation in a SIRT1-dependent manner in vitro. PMID: 27323886
    3. Findings indicate that protein inhibitor of activated STAT 4 (PIAS4) mediated SIRT1 repression in response to nutrient surplus contributes to the pathogenesis of Nonalcoholic Steatohepatitis (NASH). PMID: 27285989
    4. new protein isoform encoded by KIAA0317, termed fibrosis-inducing E3 ligase 1 (FIEL1), which potently stimulates the TGFbeta signaling pathway through the site-specific ubiquitination of PIAS4. PMID: 27162139
    5. the SUMO E3 ligase PIASy (also known as PIAS4) was induced by hypoxia and prevented Sp1 from binding to the SIRT1 promoter. PMID: 23843607
    6. PIASy negatively regulates both IFN transcription and IFN-stimulated gene expression through multiple mechanisms utilizing the function of different domains. PMID: 21199872
    7. PIASgamma as a transcriptional co-regulator of Nurr1 and suggest that this interaction may have a physiological role in regulating the expression of Nurr1 target genes. PMID: 14559918
    8. Piasy(-/-) mice appear phenotypically normal, activation of STAT1 is not significantly perturbed, and sumoylation patterns for SUMO-1 or SUMO-3 modification are similar in wild-type and knockout mice PMID: 15169916
    9. Analysis of mice carrying a targeted mutation of the Piasy gene indicates that PIASy has a modest effect on cytokine and Wnt signaling, suggesting its redundancy with other members of the PIAS family. PMID: 15528356
    10. by controlling Piasy stability, Trim32 regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB PMID: 16816390
    11. PIASy cooperates with PIAS1 to down-regulate the specificity and magnitude of NF-kappa B/STAT1-mediated gene activation. PMID: 17606919
    12. provide the first evidence for the existence of a close-spatially controlled-mode of regulation of FIP200 and PIASy nucleocytoplasmic functions PMID: 18285457
    13. Data show that poly(ADP-ribose)-polymerase-1 is the DNA proximal regulator, which senses DNA strand breaks and, through poly(ADP-ribose) synthesis, assembles assembles IKKgamma, PIASy, and ATM in a dynamic manner. PMID: 19917246

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  • 亚细胞定位：
    Nucleus, PML body. Note=Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies. Accumulates in the cytoplasm upon treatment with UVB and TNF-alpha.
  • 蛋白家族：
    PIAS family
  • 组织特异性：
    Widely expressed, with highest levels in testis. Also expressed in vascular endothelial cells, in primary keratinocytes and in the CNS, including cortex, olfactory bulb, spinal cord, thalamus and trigeminal ganglion. Low expression, if any, in liver and l
  • 数据库链接：

    KEGG: mmu:59004

    STRING: 10090.ENSMUSP00000005064

    UniGene: Mm.34428