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  4. Recombinant Mouse E3 ubiquitin-protein ligase CHFR (Chfr)

Recombinant Mouse E3 ubiquitin-protein ligase CHFR (Chfr)

  • 货号：
    CSB-YP767373MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP767373MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP767373MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP767373MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP767373MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Chfr
  • Uniprot No.：
  • 别名：
    ChfrE3 ubiquitin-protein ligase CHFR; EC 2.3.2.27; Checkpoint with forkhead and RING finger domains protein; RING-type E3 ubiquitin transferase CHFR
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-664
  • 氨基酸序列
    MELHGEEQPP PPQEPWGRLL RLGAEEDEPQ ILLWKREWTI GRRRGCDLSF PSNKLVSGDH CKLTVDEISG EVTLEDTSTN GTVINKLQVV KKQTYPLQSG DIIYLVYRKN EPEHNVAYLY ESLSGKQSLT QDSLEANKEN MFHVTKDCSG PGQGDDPQVP LLSPMAQTCL EEPQPSTSTS DLLPTASTSS TEPELTSAGQ KHSSSSGPGN TSISPKGRSS LVANGELSSL SPVFQDKEAS FSLLESKDHE ELEPAKKKMK GDGELDTNLQ LLVSGQRGNA QTSSEDVKDA SVKPDKMEET LTCIICQDLL HDCVSLQPCM HTFCAACYSG WMERSSLCPT CRCPVERICK NHILNNLVEA YLIQHPDKSR SEEDVRSMDA RNKITQDMLQ PKVRRSFSDE EGSSEDLLEL SDVDSESSDI SQPYIVCRQC PEYRRQAVQS LPCPVPESEL GATLALGGEA PSTSASLPTA APDYMCPLQG SHAICTCCFQ PMPDRRAERE QDPRVAPQQC AVCLQPFCHL YWGCTRTGCF GCLAPFCELN LGDKCLDGVL NNNNYESDIL KNYLATRGLT WKSVLTESLL ALQRGVFMLS DYRITGNTVL CYCCGLRSFR ELTYQYRQNI PASELPVTVT SRPDCYWGRN CRTQVKAHHA MKFNHICEQT RFKN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
  • 基因功能参考文献：
    1. CHFR is important for the survival of male premeiotic germ cells, which is likely through maintaining genomic stability in spermatogonial stem cells. PMID: 26566864
    2. The interaction between CHFR and PARP-1 plays an important role in cell cycle regulation and cancer therapeutic strategies. PMID: 22337872
    3. CHFR interacts with BRG1, SNF5, and BAF60a of the SWI/SNF-like BAF complex and ubiquitinates them to target for degradation through a proteasome-mediated pathway; SRG3 stabilizes these components by blocking their interaction with CHFR. PMID: 22285184
    4. RNF8 and Chfr, function together to activate ATM and maintain genomic stability in vivo. PMID: 21706008
    5. Stil protein regulates centrosome integrity and mitosis through suppression of Chfr. PMID: 21245198
    6. PML bodies control the distribution, dynamics and function of CHFR PMID: 15467728
    7. Downregulation of Chfr contributes to tumorigenesis by controlling the expression levels of Aurora A. PMID: 15793587
    8. Mice with deficiencies in both Chfr and Mlh1 (the gene that encodes the DNA mismatch-repair protein Mlh1) displayed dramatically higher incidence of spontaneous tumors relative to mice deficient for only one of these genes. PMID: 19690386

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  • 亚细胞定位：
    Nucleus, PML body.
  • 蛋白家族：
    CHFR family
  • 数据库链接：

    KEGG: mmu:231600

    STRING: 10090.ENSMUSP00000014812

    UniGene: Mm.30264