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  4. Recombinant Mouse E3 ubiquitin-protein ligase RFWD2 (Rfwd2)

Recombinant Mouse E3 ubiquitin-protein ligase RFWD2 (Rfwd2)

  • 货号：
    CSB-YP886432MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP886432MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP886432MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP886432MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP886432MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cop1; Rfwd2; RNF200E3 ubiquitin-protein ligase COP1; EC 2.3.2.27; Constitutive photomorphogenesis protein 1 homolog; mCOP1; RING finger and WD repeat domain protein 2; RING-type E3 ubiquitin transferase RFWD2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-733
  • 氨基酸序列
    MSGSRQAGSG SAGTSPGSSA ASSVTSASSS LSSSPSPPSV AASAATLVSG GVAPAAGSGG LGGPGRPVLV AAAVSGSASA GGAVSAGQSR LSCAARPSAG VGGSSSSLGS SSRKRPLLVP LCNGLLNSYE DKSNDFVCPI CFDMIEEAYM TKCGHSFCYK CIHQSLEDNN RCPKCNYVVD NIDHLYPNFL VNELILKQKQ RFEEKRFKLD HSVSSTNGHR WQIFQDLLGT DQDNLDLANV NLMLELLVQK KKQLEAESHA AQLQILMEFL KVARRNKREQ LEQIQKELSV LEEDIKRVEE MSGLYSPVSE DSTVPQFEAP SPSHSSIIDS TEYSQPPGFS GTSQTKKQPW YNSTLASRRK RLTAHFEDLE QCYFSTRMSR ISDDSRTASQ LDEFQECLSK FTRYNSVRPL ATLSYASDLY NGSSIVSSIE FDRDCDYFAI AGVTKKIKVY EYGTVIQDAV DIHYPENEMT CNSKISCISW SSYHKNLLAS SDYEGTVILW DGFTGQRSKV YQEHEKRCWS VDFNLMDPKL LASGSDDAKV KLWSTNLDNS VASIEAKANV CCVKFSPSSR YHLAFGCADH CVHYYDLRNT KQPIMVFKGH RKAVSYAKFV SGEEIVSAST DSQLKLWNVG KPYCLRSFKG HINEKNFVGL ASNGDYIACG SENNSLYLYY KGLSKTLLTF KFDTVKSVLD KDRKEDDTNE FVSAVCWRAL SDGESNVLIA ANSQGTIKVL ELV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif.
  • 基因功能参考文献：
    1. Prenatal inactivation of Rfwd2 gene in the lung epithelium led to a striking halt in branching morphogenesis shortly after secondary branch formation. PMID: 27335464
    2. Study shows that post-translational regulation of the transcription factors ETV1, ETV4, and ETV5 by the ubiquitin ligase COP1 (also called RFWD2) in beta cells is critical for insulin secretion. Mice lacking COP1 in beta cells developed diabetes due to insulin granule docking defects that were fully rescued by genetic deletion of Etv1, Etv4, and Etv5. PMID: 26627735
    3. These results indicate that COP1 and Trib1 act as an oncoprotein complex functioning upstream of C/EBPalpha, and its ligase activity is crucial for leukemogenesis. PMID: 23884858
    4. COP1 physically interacted with PTP1B and suppressed PTP1B phosphatase activity as well as the association of PTP1B with IRbeta. PMID: 23439647
    5. Modulation of fatty acid synthase degradation by concerted action of p38 MAP kinase, E3 ligase COP1, and SH2-tyrosine phosphatase Shp2. PMID: 23269672
    6. the ubiquitin ligase COP1 (also known as RFWD2) is a tumour suppressor that negatively regulates ETV1, ETV4 and ETV5; ETV1, which is mutated in prostate cancer more often, was degraded after being ubiquitinated by COP1 PMID: 21572435
    7. Cop1 is a tumor suppressor that functions, at least in part, by antagonizing c-Jun oncogenic activity. PMID: 21403399
    8. Rfwd2 is associated with acute lung injury PMID: 21297076
    9. description of a pathway in which Tribbles 3 (TRB3) stimulates lipolysis by triggering the degradation of acetyl-coenzyme A carboxylase (ACC) in adipose tissue; TRB3 promoted ACC ubiquitination through an association with the E3 ubiquitin ligase COP1 PMID: 16794074
    10. Disruption of the COP1-mediated proteolysis by ionizing radiation leads to MTA1 stabilization. PMID: 19805145

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  • 亚细胞定位：
    Nucleus speckle. Cytoplasm. Note=In the nucleus, it forms nuclear speckles.
  • 蛋白家族：
    COP1 family
  • 数据库链接：

    KEGG: mmu:26374

    STRING: 10090.ENSMUSP00000076160

    UniGene: Mm.328135