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  4. Recombinant Mouse E3 ubiquitin-protein ligase RING2 (Rnf2)

Recombinant Mouse E3 ubiquitin-protein ligase RING2 (Rnf2)

  • 中文名称：
    小鼠Rnf2重组蛋白
  • 货号：
    CSB-YP880405MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rnf2重组蛋白
  • 货号：
    CSB-EP880405MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rnf2重组蛋白
  • 货号：
    CSB-EP880405MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rnf2重组蛋白
  • 货号：
    CSB-BP880405MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rnf2重组蛋白
  • 货号：
    CSB-MP880405MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rnf2
  • Uniprot No.：
  • 别名：
    Rnf2; DinG; Ring1b; E3 ubiquitin-protein ligase RING2; EC 2.3.2.27; RING finger protein 1B; RING1b; RING finger protein 2; RING-type E3 ubiquitin transferase RING2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-336
  • 氨基酸序列
    SQAVQTNGT QPLSKTWELS LYELQRTPQE AITDGLEIVV SPRSLHSELM CPICLDMLKN TMTTKECLHR FCADCIITAL RSGNKECPTC RKKLVSKRSL RPDPNFDALI SKIYPSRDEY EAHQERVLAR INKHNNQQAL SHSIEEGLKI QAMNRLQRGK KQQIENGSGA EDNGDSSHCS NASTHSNQEA GPSNKRTKTS DDSGLELDNN NAAVAIDPVM DGASEIELVF RPHPTLMEKD DSAQTRYIKT SGNATVDHLS KYLAVRLALE ELRSKGESNQ MNLDTASEKQ YTIYIATASG QFTVLNGSFS LELVSEKYWK VNKPMELYYA PTKEHK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. Plays a role in the transcriptional repression of genes that are required for pluripotency in embryonic stem cells, thereby contributing to differentiation of the ectodermal and endodermal germ layers. Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes. Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation.
  • 基因功能参考文献：
    1. Data show that RNF2 promotes STAT1/STAT2 disassociation from DNA. PMID: 29242538
    2. the ability of RING1B, a core component of PRC1, to ubiquitinate histone H2A is dispensable for early mouse embryonic development and much of the gene repression activity of PRC1 PMID: 26385961
    3. RNF2 associates with AMBRA1 to act as an E3 ligase to ubiquitinate AMBRA1 via K48 linkage. PMID: 24980959
    4. The polycomb component Ring1B regulates the timed termination of Fezf2 expression and thus subcerebral projection neuron production during mouse neocortical development. PMID: 25344075
    5. Expansion of hepatic stem/progenitor cells requires Ring1B-mediated epigenetic silencing of Cdkn1a and Cdkn2a, demonstrating that Ring1B simultaneously regulates multiple CDKIs in tissue stem/progenitor cells. PMID: 24497168
    6. Af9 mediates site-selective physical and functional recruitment of Rnf2 to the alpha-ENaC promoter to constrain basal alpha-ENaC transcription in collecting duct cells. PMID: 24070375
    7. Ring1b mediates Meis2 promoter and midbrain-specific enhancer association. PMID: 24374176
    8. Aurora B and Ring1B co-occupy active promoters in resting lymphocytes. PMID: 24034696
    9. Fbxl10 has a key role in regulating Ring1B recruitment to its target genes and H2AK119 ubiquitylation in embryonic stem cells. PMID: 23395003
    10. Ring1b inactivation in differentiated islet beta cells (Ring1b(betaKO)) did not cause derepression, even after multiple rounds of cell division, suggesting a role for Ring1b in the establishment but not the maintenance of repression PMID: 23271347
    11. It was shown that Ring1B interacted with multiple complexes in embryonic stem cells. Although H2A.Z co-localized with Eed, Ring1B and CpG islands in chromatin, H2A.Z still blanketed polycomb target loci in the absence of Suz12, Eed, or Ring1B. PMID: 22496869
    12. Data provide a valuable basis for future investigations aimed at eukaryotic DING proteins, revealing that these proteins seem ubiquitous in mouse tissue. PMID: 20161715
    13. Ring1B and Suv39h1 have mutually exclusive roles in regulating distinct chromatin states at key developmental genes, and play a part in how lineage specification can be reinforced during early development PMID: 20573702
    14. Ring1B-mediated chromatin compaction acts to directly limit transcription in embryonic stem cells. PMID: 20471950
    15. polycomb protein Ring1B regulation by self-ubiquitination or by E6-AP may have implications to the pathogenesis of Angelman syndrome PMID: 20351251
    16. Involved in the specification of the anterior-posterior axis in mice PMID: 12183370
    17. Rnf2 has a role in mouse gastrulation and cell cycle regulation PMID: 12589020
    18. Ring1b and ubH2A are involved in the initiation of both imprinted and random X inactivation PMID: 15509584
    19. Sf3b1-Rnf2 protein interaction is essential for true Polycomb group proteins mediated repression of Hox genes. PMID: 15741318
    20. Study using a high throughput proteomics approach identified interactors with Rnf2 including Fbxl10/Jhdm1B, casein kinase subunits, and the BcoR corepressor PMID: 17296600
    21. Ring1B is crucial for the regulation of developmental control genes and PRC1 proteins but not X inactivation in embryonic cells. PMID: 17620408
    22. C-terminal domain of Ring1B is a dimer made of stable, well-structured monomers. PMID: 17935356
    23. Ring1B restricts the progenitors' proliferation and promotes the proliferation of their maturing progeny by selectively altering the expression pattern of cell cycle regulators along hematopoietic differentiation PMID: 18039844
    24. Ring1b is essential to stably maintain an undifferentiated state of mouse ES cells by repressing genes with important roles during differentiation and development PMID: 18493325
    25. The Polycomb group proteins Ezh2 and Rnf2 are independently required for genomic contraction and imprinted silencing. PMID: 18848501
    26. Data suggest a role for Ring1B in maintenance of the undifferentiated state of embryonic neural stem/progenitor cells. They also suggest that Ring1B may modulate the differentiation potential of NSCs to neurons and glia. PMID: 19544461

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Chromosome.
  • 组织特异性：
    Expressed in embryonic stem cells.
  • 数据库链接：

    KEGG: mmu:19821

    STRING: 10090.ENSMUSP00000075476

    UniGene: Mm.474038