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  4. Recombinant Mouse E3 ubiquitin-protein ligase pellino homolog 1 (Peli1)

Recombinant Mouse E3 ubiquitin-protein ligase pellino homolog 1 (Peli1)

  • 中文名称：
    小鼠Peli1重组蛋白
  • 货号：
    CSB-YP804464MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Peli1重组蛋白
  • 货号：
    CSB-EP804464MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Peli1重组蛋白
  • 货号：
    CSB-EP804464MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Peli1重组蛋白
  • 货号：
    CSB-BP804464MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Peli1重组蛋白
  • 货号：
    CSB-MP804464MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Peli1
  • Uniprot No.：
  • 别名：
    Peli1; E3 ubiquitin-protein ligase pellino homolog 1; Pellino-1; EC 2.3.2.27; RING-type E3 ubiquitin transferase pellino homolog 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-418
  • 氨基酸序列
    MFSPDQENHP SKAPVKYGEL IVLGYNGSLP NGDRGRRKSR FALFKRPKAN GVKPSTVHIA CTPQAAKAIS NKDQHSISYT LSRAQTVVVE YTHDSNTDMF QIGRSTESPI DFVVTDTVPG SQSNSDTQSV QSTISRFACR IICERSPPFT ARIYAAGFDS SKNIFLGEKA AKWKTSDGQM DGLTTNGVLV MHPRNGFTED SKPGIWREIS VCGNVFSLRE TRSAQQRGKM VEIETNQLQD GSLIDLCGAT LLWRTAEGLS HTPTVKHLEA LRQEINAARP QCPVGFNTLA FPSMKRKDVV DEKQPWVYLN CGHVHGYHNW GNKEERDGKD RECPMCRSVG PYVPLWLGCE AGFYVDAGPP THAFSPCGHV CSEKTTAYWS QIPLPHGTHT FHAACPFCAH QLAGEQGYIR LIFQGPLD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation. Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3. Negatively regulates necroptosis by reducing RIPK3 expression. Mediates 'Lys-63'-linked ubiquitination of RIPK1.
  • 基因功能参考文献：
    1. Peli1 has a B cell-intrinsic function to protect against lupus-like autoimmunity. Peli1 deficiency in B cells induces autoantibody production via noncanonical NF-kappaB signaling. Mechanically, Peli1 functions as an E3 ligase to associate with NF-kappaB inducing kinase (NIK) and mediates NIK Lys48 ubiquitination and degradation. PMID: 29555915
    2. This study demonstrates Pellino 1 (PELI1) as an important modulator that exerts opposite regulatory functions on apoptosis and necroptosis, two distinct forms of regulated cell death mechanisms. PMID: 29078411
    3. Peli1 is a proangiogenic molecule that acts downstream of VEGF-Flk-1 and restores angiogenesis and enhances skin flap survivability. PMID: 28177171
    4. a novel miR-155-Peli1-c-Rel pathway that specifically regulates Tfh cell generation and function. PMID: 27481129
    5. Pellino1 plays an important role in the pathogenesis of myocardial infarction PMID: 24442869
    6. The nonredundant role of Pellino1 in immune receptor signaling is in part due to its different substrate specificity. PMID: 25027698
    7. Pellino1 interacts with the transcription factor Deformed Epidermal Autoregulatory Factor 1 (DEAF1). PMID: 23846693
    8. These results demonstrate that the ligands that signal via MyD88 do not always employ the same protein kinase to activate Pellino 1 and show that the activiation of NF-kappaB and mitogen-activated protein kinases. PMID: 22007846
    9. Peli1 as a critical factor in the maintenance of peripheral T cell tolerance and demonstrate a previously unknown mechanism of c-Rel regulation. PMID: 21874024
    10. Pellino-1 protein may have a cellular function distinct from previously identified functions. PMID: 21120624
    11. miR-21 expression was upregulated during early stages of liver regeneration. Targeting of Peli1 by miR-21 could potentially provide the basis for a negative feedback cycle regulating NF-kappaB signaling. PMID: 20167875
    12. Peli1 is a ubiquitin ligase needed for the transmission of TRIF-dependent TLR signals. PMID: 19734906

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  • 蛋白家族：
    Pellino family
  • 数据库链接：

    KEGG: mmu:67245

    STRING: 10090.ENSMUSP00000090979

    UniGene: Mm.28957