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  4. Recombinant Mouse EGF-containing fibulin-like extracellular matrix protein 2 (Efemp2)

Recombinant Mouse EGF-containing fibulin-like extracellular matrix protein 2 (Efemp2)

  • 货号：
    CSB-YP007451MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007451MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007451MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007451MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007451MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Efemp2
  • Uniprot No.：
  • 别名：
    Efemp2; Fbln4; Mbp1; EGF-containing fibulin-like extracellular matrix protein 2; Fibulin-4; FIBL-4; Mutant p53-binding protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    26-443
  • 氨基酸序列
    SPQDP EEPDSYTECT DGYEWDADSQ HCRDVNECLT IPEACKGEMK CINHYGGYLC LPRSAAVISD LHGEGPPPPA AHAQQPNPCP QGYEPDEQES CVDVDECTQA LHDCRPSQDC HNLPGSYQCT CPDGYRKIGP ECVDIDECRY RYCQHRCVNL PGSFRCQCEP GFQLGPNNRS CVDVNECDMG APCEQRCFNS YGTFLCRCNQ GYELHRDGFS CSDIDECGYS SYLCQYRCVN EPGRFSCHCP QGYQLLATRL CQDIDECETG AHQCSEAQTC VNFHGGYRCV DTNRCVEPYV QVSDNRCLCP ASNPLCREQP SSIVHRYMSI TSERSVPADV FQIQATSVYP GAYNAFQIRS GNTQGDFYIR QINNVSAMLV LARPVTGPRE YVLDLEMVTM NSLMSYRASS VLRLTVFVGA YTF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a crucial role in elastic fiber formation in tissue, and in the formation of ultrastructural connections between elastic laminae and smooth muscle cells in the aorta, therefore participates in terminal differentiation and maturation of smooth muscle cell (SMC) and in the mechanical properties and wall integrity maintenance of the aorta. In addition, is involved in the control of collagen fibril assembly in tissue throught proteolytic activation of LOX leading to cross- linking of collagen and elastin. Also promotes ELN coacervation and participates in the deposition of ELN coacervates on to microfibrils but also regulates ELN cross- linking through LOX interaction. Moreover adheres to the cells through heparin binding in a calcium-dependent manner and regulates vascularlar smooth muscle cells proliferation through angiotensin signaling.
  • 基因功能参考文献：
    1. These data clearly establish a functional link between Ltbp-4L and fibulin-4 as a crucial molecular requirement for survival and elastogenesis in mice. PMID: 27585882
    2. fibulin-4 seems to play a role in regulating tendon collagen fibrillogenesis, in addition to its essential function in elastogenesis. PMID: 26711913
    3. Findings suggested that fibulin-4 is important for the proteolytic activation of lysyl oxidase which has a pivotal role in cross-linking of collagen and elastin. PMID: 26690653
    4. Fibulin-4 serves as a potential scaffolding protein during collagen maturation in the extracellular space and loss of fibulin-4 disrupts collagen synthesis and maturation. PMID: 26220971
    5. fibulin-4 plays a role in regulating collagen fibril assembly and offer a preclinical platform for developing treatments for autosomal recessive cutis laxa 1B. PMID: 26178373
    6. MBP1 inhibits Ab fibril formation in vitro and demonstrate the ability of MBP1 to reduce Ab pathology and improve behavioral performance. PMID: 25457550
    7. pulmonary emphysema in aneurysmal Fibulin-4 deficient (Fibulin-4(R)) mice PMID: 25255451
    8. Smooth muscle cell (SMC)-specific deletion of Fibulin-4 knockout ascending aorta and carotid artery showed mechanical changes in the axial direction. PMID: 24526456
    9. Compared to control SMCs, the modulus of Eln-/- SMCs is reduced by 40%, but is unchanged in Fbln4-/- SMCs. The Eln-/- SMC modulus is rescued by soluble or alpha elastin treatment. PMID: 24322348
    10. abnormal up-regulation of angiotensin-converting enzyme (ACE) in SMCs and subsequent activation of angiotensin II (AngII) signaling are involved in the onset of aortic aneurysms in Fbln4 mice. PMID: 23636094
    11. Data show that fibulin-4 staining intensity in the ligamentum flavum was greatest at P14, though moderately strong at P7 and P21, and efore P7 and after P28, staining was detectable but relatively minimal. PMID: 22685574
    12. Report coregistration of MMP activity by noninvasive tomographic imaging methods allows the detection of increased MMP activity, even before the aneurysm has actually formed in fibulin-4 deficient mice. PMID: 20592247
    13. Fibulin-4 plays a dual role in the formation of elastic fibers as well as terminal differentiation and maturation of SMCs in the aortic wall. PMID: 20019329
    14. fibulin-4 plays an irreplaceable role in elastogenesis. PMID: 16478991
    15. A sufficient amount of FBLN4 is essential for tethering LOX to tropoelastin to facilitate cross-linking during elastogenesis. PMID: 19855011

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, extracellular matrix, basement membrane.
  • 蛋白家族：
    Fibulin family
  • 组织特异性：
    Expressed in elastic fibers of the skin, near the dermal-epidermal junction, surrounding the hair follicles and throughout the dermis. Expressed in tendon around tenocytes. Prominently expressed in cartilage, bone, perichondrium and ligaments. Also detect
  • 数据库链接：

    KEGG: mmu:58859

    STRING: 10090.ENSMUSP00000064719

    UniGene: Mm.276367