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  4. Recombinant Mouse Endonuclease 8-like 1 (Neil1)

Recombinant Mouse Endonuclease 8-like 1 (Neil1)

  • 货号：
    CSB-YP809118MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP809118MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP809118MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP809118MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP809118MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Neil1; Nei1Endonuclease 8-like 1; EC 3.2.2.-; EC 4.2.99.18; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease VIII-like 1; Nei homolog 1; NEH1; Nei-like protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-389
  • 氨基酸序列
    PEGPELHLA SHFVNETCKG LVFGGCVEKS SVSRNPEVPF ESSAYHISAL ARGKELRLTL SPLPGSQPPQ KPLSLVFRFG MSGSFQLVPA EALPRHAHLR FYTAPPAPRL ALCFVDIRRF GHWDPGGEWQ PGRGPCVLLE YERFRENVLR NLSDKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARTVLEA LQQCRPSPEL TLSQKIKAKL QNPDLLELCH LVPKEVVQLG GKGYGPERGE EDFAAFRAWL RCYGVPGMSS LRDRHGRTIW FQGDPGPLAP KGGRSQKKKS QETQLGAEDR KEDLPLSSKS VSRMRRARKH PPKRIAQQSE GAGLQQNQET PTAPEKGKRR GQRASTGHRR RPKTIPDTRP REAGESSAS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.
  • 基因功能参考文献：
    1. Loss of Neil1 expression is associated with hepatocellular carcinoma. PMID: 28373545
    2. Collectively the data suggest that NEIL1-initiated repair of a subset of ROS-induced DNA base lesions may be insufficient to prevent the initiation of inflammatory pathways during chronic UV exposure in mouse skin. PMID: 27818081
    3. Its loss causes defects in olfactory function. PMID: 25448603
    4. Rad9 regulates base excision repair by controlling Neil1 protein stability in mouse embryonic stem cells. PMID: 25873625
    5. Hypersensitivity to H2O2 caused by NEIL1 knockdown was more significant in S phase than in G1 phase, suggesting that NEIL1 has an important role during S phase. PMID: 24706997
    6. one role for Neil3 and NEIL1 is to repair DNA base damages in telomeres in vivo and that Neil3 and Neil1 may function in quadruplex-mediated cellular events, such as gene regulation via removal of damaged bases from quadruplex DNA. PMID: 23926102
    7. binds to the BRCT domain of PARP-1 PMID: 23104860
    8. Neil1 contributes to germline and somatic Huntington's disease CAG repeat expansion. PMID: 22914735
    9. Endonuclease VIII-like 1 (NEIL1) promotes short-term spatial memory retention and protects from ischemic stroke-induced brain dysfunction and death in mice. PMID: 22927410
    10. These results suggest that mNEIL1 mRNA variants are expressed in a variety of organs in normal mice and that variant 1 protein may regulate mNEIL1 activity. PMID: 22510596
    11. NEIL1 deficiency results in an increased susceptibility to obesity and related complications potentially by lowering the threshold for tolerance of cellular oxidative stress in neil1(-/-) mice. PMID: 21285402
    12. NEIL1 is involved in nucleotide excision repair of(5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines, in addition to its function as a DNA glycosylase in base excision repair. PMID: 20067321
    13. NEIL1 is a back-up glycosylase for NTH1 with unique substrate specificity and tissue-specific expression. PMID: 12200441
    14. repression of Neil1 espression by RNA interference PMID: 12536207
    15. Neil1 is an essential component of base excision repair in mammalian cells; its presence may contribute to the redundant repair capacity observed in Ogg1 -/- and Nth1 -/- mice PMID: 12713815
    16. ability of Neil1 to discriminate between thymine glycol stereoisomers PMID: 14726482
    17. NEIL1 and NEIL2 are essential in the recognition of oxidized lesions arising from 8-oxoG and may play important roles in the repair of DNA damage induced by carcinogenic metals. PMID: 15533836
    18. Measurement of excision kinetics showed that mNEIL1 possesses equal specificity for 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 4,6-diamino-5-formamidopyrimidine. PMID: 15595846
    19. identified nei endonuclease VIII-like 1(NEIL1) in liver mitochondria, which could account for the residual DNA repair activity in the absence of OGG1 and NTH1 PMID: 16221681
    20. suggest an important role for NEIL1 in the prevention of the diseases associated with the metabolic syndrome PMID: 16446448
    21. Results suggest a role for NEIL1 and 2 as backup deoxyribo-5'-phosphate lyases in mammalian cells. PMID: 16920106
    22. The high incidence of tumors in Nth1-/-Neil1-/- mice together with the activating mutation in the K-ras gene of their pulmonary tumors, reveal for the first time, the existence of mutagenic and carcinogenic oxidative damage to DNA which is not 8-OH-Gua. PMID: 19346169
    23. found that Neil1 was highly constitutively expressed in the germinal center B cells, a rapidly dividing cell population that is undergoing immunoglobulin gene hypermutation and isotype switching. PMID: 19782007

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Chromosome.
  • 蛋白家族：
    FPG family
  • 组织特异性：
    Detected in heart, spleen and lung.
  • 数据库链接：

    KEGG: mmu:72774

    STRING: 10090.ENSMUSP00000034842

    UniGene: Mm.35749