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  4. Recombinant Mouse Endonuclease III-like protein 1 (Nthl1)

Recombinant Mouse Endonuclease III-like protein 1 (Nthl1)

  • 货号：
    CSB-YP016125MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016125MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016125MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016125MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016125MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Nthl1; Nth1; Endonuclease III-like protein 1; EC 3.2.2.-; EC 4.2.99.18; Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase; DNA glycosylase/AP lyase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-300
  • 氨基酸序列
    MNSGVRMVTR SRSRATRIAS EGCREELAPR EAAAEGRKSH RPVRHPRRTQ KTHVAYEAAN GEEGEDAEPL KVPVWEPQNW QQQLANIRIM RSKKDAPVDQ LGAEHCYDAS APPKVRRYQV LLSLMLSSQT KDQVTAGAMQ RLRARGLTVE SILQTDDDTL GRLIYPVGFW RNKVKYIKQT TAILQQRYEG DIPASVAELV ALPGVGPKMA HLAMAVAWGT ISGIAVDTHV HRIANRLRWT KKMTKTPEET RKNLEEWLPR VLWSEVNGLL VGFGQQICLP VHPRCQACLN KALCPAAQDL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.
  • 基因功能参考文献：
    1. These results suggest that Nth1 plays an important role in telomere maintenance and base repair against oxidative stress-induced base modifications. PMID: 23874233
    2. deficiencies in Ogg1 and/or Nth1 do not lead to decreased DNA double strand breaks PMID: 22536455
    3. To determine the significance of thymine glycol repair in mammals, a mouse model with mutated mNth1, a homolog of nth, was made by gene targeting. Mutant mouse liver, instead of mNTH1 activity, had at least 2 novel DNA glycosylase activities against Tg. PMID: 12093749
    4. To determine the role of murine Nth1 in protecting genomic integrity, a knockout strain was created. No phenotypic changes from wild type were noted due to a novel AP endonuclease DNA repair system. PMID: 12167705
    5. NEIL1 is a back-up glycosylase for NTH1 with unique substrate specificity and tissue-specific expression PMID: 12200441
    6. the mechanism for the removal of 5,6-dihydrouracil from DNA by mNTH1-deficient cell-free extracts derived from testes of mNTH1 knock-out mice PMID: 12401779
    7. mNTH1 is responsible for the repair of thymine glycols in mitochondrial DNA PMID: 12819227
    8. ability of nth1 to discriminate between thymine glycol stereoisomers PMID: 14726482
    9. results indicated that 8-oxoguanine DNA-glycosylase (OGG1) and nth (endonuclease III)-like 1(NTH1) are the major DNA glycosylases for the removal of 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG) and 4,6-diamino-5-formamidopyrimidine (FapyA) PMID: 16221681

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  • 亚细胞定位：
    Nucleus. Mitochondrion.
  • 蛋白家族：
    Nth/MutY family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    KEGG: mmu:18207

    STRING: 10090.ENSMUSP00000047413

    UniGene: Mm.148315