Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Epidermal growth factor-like protein 8 (Egfl8)

Recombinant Mouse Epidermal growth factor-like protein 8 (Egfl8)

In Stock
  • 中文名称：
    小鼠Egfl8重组蛋白
  • 货号：
    CSB-EP753592MO
  • 规格：
    ￥1536
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Egfl8
  • Uniprot No.：
  • 别名：
    Egfl8; Ng3Epidermal growth factor-like protein 8; EGF-like protein 8
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    49.0 kDa
  • 表达区域：
    29-293aa
  • 氨基酸序列
    GSFKESLGVCSKQTLLVPLRYNESYSQPVYKPYLTLCAGRRICSTYRTTYRVAWREVRREVPQTHVVCCQGWKKPHPGALTCDAICSKPCLNGGVCTGPDRCECAPGWGGKHCHVDVDECRASLTLCSHGCLNTLGSFLCSCPHPLVLGLDGRTCAGGPPESPTSASILSVAVREADSEEERALRWEVAELRGRLEKLEQWATQAGAWVRAVLPMPPEELRPEQVAELWGRGDRIESLSDQVLLLEERLGACACEDNSLGPSLRG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    This recombinant Mouse Egfl8 protein is typically achieved by the manipulation of Egfl8 gene expression in E.coli cells so that it expresses large amounts of a recombinant Egfl8 gene. In order to get enough amount of the Egfl8 protein, strain selection, codon optimization, fusion systems, co-expression, mutagenesis, and isotope labeling techniques are used. Finally, the Egfl8 protein is isolated from the samples such as cell lysates or medium. Protein refolding, cleavage of fusion moieties and chromatography techniques are involved in the protein purification process.

    Egfl8 plays negative regulatory roles in mouse thymic epithelial cells (TECs) and thymocytes. It has shown that Egfl8 is downregulated in gastric cancer (GC) and is closely associated with high tumor-node-metastasis stage and dismal prognosis in gastric and colorectal cancer. In addition to participating in thymopoiesis, Egfl8 is also involved in cell migration and invasion by regulating Notch signaling. Egfl8 also can act as a neuritogen and rewire cellular signaling by activating kinases involved in neurogenesis.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 基因功能参考文献：
    1. Mouse EGFL8 encodes a protein of 293 amino acids with two EGFL domains, an Emilin-like domain and a Ca(2+)-binding EGFL domain, which has a molecular mass of 32 kDa. PMID: 26096343
    2. EGFL8 inhibited thymocyte proliferation and induced thymocyte apoptosis. PMID: 23877103
    3. these data show that EGFL8 exerts inhibitory effects on thymic epithelial cells and thymocytes, suggesting that EGFL8 acts as a negative regulatory molecule in the development of T cells in the mouse thymus. PMID: 22828506
  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Ubiquitously expressed in brain, kidney, thymus and lung.
  • 数据库链接：

    KEGG: mmu:81701

    STRING: 10090.ENSMUSP00000015611

    UniGene: Mm.231343