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  4. Recombinant Mouse Erythroid transcription factor (Gata1)

Recombinant Mouse Erythroid transcription factor (Gata1)

  • 货号：
    CSB-YP009274MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009274MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009274MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009274MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009274MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gata1
  • Uniprot No.：
  • 别名：
    Gata1; Gf-1Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-413
  • 氨基酸序列
    MDFPGLGALG TSEPLPQFVD SALVSSPSDS TGFFSSGPEG LDAASSSTSP NAATAAASAL AYYREAEAYR HSPVFQVYPL LNSMEGIPGG SPYASWAYGK TALYPASTVC PSHEDAPSQA LEDQEGKSNN TFLDTLKTER LSPDLLTLGT ALPASLPVTG SAYGGADFPS PFFSPTGSPL SSAAYSSPKF HGSLPLAPCE ARECVNCGAT ATPLWRRDRT GHYLCNACGL YHKMNGQNRP LIRPKKRMIV SKRAGTQCTN CQTTTTTLWR RNASGDPVCN ACGLYFKLHQ VNRPLTMRKD GIQTRNRKAS GKGKKKRGSN LAGAGAAEGP AGGFMVVAGS SSSGNCGEVA SGLALGTAGT AHLYQGLGPV VLSGPVSHLM PFPGPLLGSP TTSFPTGPAP TTSSTSVIAP LSS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.
  • 基因功能参考文献：
    1. GATA1 interacts with the STING promoter and activates the basal transcription of the STING gene. PMID: 28522827
    2. GATA1 may be a potential regulator of Six2-maintained population of nephron progenitor cells. PMID: 28842839
    3. Gata1(low) mice are a bona fide model of MF, which recapitulates the hyperactivation of the TPO/MPL/JAK2 axis observed in megakaryocytes from myelofibrotic patients. PMID: 28622305
    4. the Gata-1 gene was a T3-directly regulated gene and that TRa1PV could impair erythropoiesis via repression of the Gata-1 gene and its regulated genes. These results provide new insights into how TRa1 mutants acted to cause erythroid abnormalities in patients with mutations of the THRA gene. PMID: 28910278
    5. Gain of function with GATA1 restores the common myeloid precursor differentiation into erythroblasts that was decreased by burn injury. There is a reciprocal relationship between myeloid-specific MafB and erythroid-specific GATA1 in these cells. MafB plays a role in dampening GATA1. PMID: 26992433
    6. expression of GATA1 effectively rescued maturation of Primary myelofibrosis megakaryocytes. PMID: 28240607
    7. Gata1-KO(DC) DCs have reduced polysialic acid levels on their surface, which is a known determinant for the proper migration of DCs toward CCL21. PMID: 27815426
    8. removal of the the Gata1 methylation-determining region -mediated silencing machinery is the molecular basis of the initial activation of the Gata1 gene and erythropoiesis. PMID: 28069743
    9. Results show that GATA1 recognizes a single GATA motif or a composite of adjacent GATA motifs and exerts its diversified bindings. These binding configurations serve as one of the critical determinants of specific transcriptional regulation. PMID: 27215385
    10. deletion of P-sel disrupted megakaryocyte/neutrophil interactions in spleen, reduced TGF-beta content, and corrected the hematopoietic stem cells distribution that in Gata1(low) mice, as in primary myelofibrosis patients, is abnormally expanded in spleen. PMID: 26439305
    11. findings suggest that Gata1 & PU.1 transcription factors are only executing and reinforcing lineage choice once made. These results challenge the current prevailing model of early myeloid lineage choice PMID: 27411635
    12. GATA1 and PU.1 bind in vitro and in vivo the proximal promoter region of the RPS19 gene which is frequently mutated in Diamond-Blackfan Anemia. PMID: 26447946
    13. TAL1 and GATA1 form a precisely organized complex at a compound motif consisting of a TG 7 or 8 bp upstream of a WGATAA motif across thousands of genomic locations PMID: 26503782
    14. immunoprecipitation. Our data provide new insights into the expanded regulatory circuitry that coordinates Nanog expression. PMID: 26296469
    15. Scrib is a novel proinflammatory regulator in endothelial cells, which maintains the protein expression of GATA-like protein-1. PMID: 26205961
    16. Inducible Gata1 suppression expands megakaryocyte-erythroid progenitors from embryonic stem cells PMID: 25961454
    17. By ablating TAF10 specifically in erythroid cells in vivo, observed is a differentiation block accompanied by deregulated GATA1 target genes, including Gata1 itself, suggesting functional cross talk between GATA1 and TAF10. PMID: 25870109
    18. GATA-1 deficiency can partially rescue the trabecular bone loss observed with osteoprotegerin deficiency PMID: 25205203
    19. the dbGATA motif has a role in the maintenance of Gata1 expression in hematopoietic progenitors PMID: 25535330
    20. Genetic deletion of the GATA1-regulated protein alpha-synuclein reduces oxidative stress and nitric oxide synthase levels in mature erythrocytes. PMID: 25043722
    21. PSTPIP2 dysregulation contributes to aberrant terminal differentiation in GATA-1-deficient megakaryocytes by activating LYN. PMID: 24407241
    22. The results show that all three elements independently and cooperatively contribute to G1HRD activity in vivo in definitive erythropoiesis, although these are contributing redundantly to primitive erythropoiesis. PMID: 24118212
    23. preceding binding of GATA2 helps the following binding of GATA1 and thereby secures smooth expression of the transient-phase genes. PMID: 23911012
    24. GATA2 plays a more important role than GATA1 in the regulation of most mast cell-specific genes. PMID: 24615013
    25. The 3.2-kb sequences interspaced between the Gata1 hematopoietic enhancer & the double GATA-motif were able to recruit DNA methyltransferase 1, exerting a cis-repressive function in hematopoietic stem cells. PMID: 24021675
    26. GATA-1 plays an important role in both generation and activation of basophils. PMID: 24167252
    27. results suggest that EPO-induced transient phosphorylation of GATA-1 at serine(2) is dispensable for erythropoiesis PMID: 23717580
    28. Data indicate a vital link between GATA-1 and the histone H4 lysine 20 methyltransferase PR-Set7/SetD8 (SetD8). PMID: 23959865
    29. Although none of the parameters measured were different between the NF-E2 deficient and control mice, an increase in calcium (21%) and an increase in the mineral/matrix ratio (32%) was observed in GATA-1 deficient mice. PMID: 23359245
    30. This study demonstrates that Ikaros directly interacts with GATA1, GATA2, and GATA3 as well as Cdk9/P-TEFb through specific protein domains. PMID: 23732910
    31. The results suggest that GATA-1 associates preferentially with changes of specific epigenetic modifications, such as H4K16, H3K27 acetylation and H3K4 di-methylation. PMID: 23519611
    32. An important functional interplay between Ikaros, GATA factors, and the NOTCH signaling pathway in specification and homeostasis of the megakaryocyte lineage. PMID: 23335373
    33. GATA-1, in the absence of FOG-1, occupies GATA-1(V205G)-selective sites. PMID: 22771118
    34. Erythropoietin regulates GATA1 through protein kinase D activation, promoting histone deacetylase 5 dissociation from GATA1, and subsequent GATA1 acetylation. PMID: 22983445
    35. Gata2 mRNA level was significantly increased while Gata1 was maintained at low levels during the differentiation of mast cells derived from mouse bone marrow. PMID: 22988301
    36. results describe a mechanism whereby GATA-1 utilizes Ikaros and Polycomb repressive complex 2 to promote Hes1 repression as an important step in erythroid cell differentiation PMID: 22778136
    37. Mitosis-specific destruction of GATA1 delays reactivation selectively of genes that retain GATA1 during mitosis; studies suggest a requirement of mitotic "bookmarking" by GATA1 for the faithful propagation of cell-type-specific transcription programs through cell division. PMID: 22901805
    38. GATA1 has two independent transactivation domains, N-TAD and C-TAD. Both N-TAD and C-TAD retain redundant as well as specific activities for proper hematopoiesis in vivo. PMID: 22556427
    39. GATA1-HS2 enhancer allows persistent and position-independent expression of a beta-globin transgene PMID: 22164220
    40. GATA factors GATA-1 and GATA-2 bind overlapping sets of genes, often at distinct sites, as a means to differentially regulate target gene expression and to regulate the balance between proliferation and differentiation. PMID: 22383799
    41. Mature erythrocyte membrane homeostasis is compromised by loss of the GATA1-FOG1 interaction resulting in thrombocytopenia and anemia. PMID: 22279059
    42. ZNF268 is a crucial downstream target and effector of GATA-1 PMID: 22235304
    43. GATA-1, SCL, and Klf1 form an erythroid core transcriptional network by co-occupying >300 genes PMID: 22357756
    44. GATA-1 is specifically required for erythrocyte-lineage commitment. PMID: 22068055
    45. c-Myb and GATA-1 act in concert to achieve correct megakaryocytic differentiation. PMID: 21668739
    46. Disruption of the GATA1/FOG1/NuRD transcriptional system results in a complex, pleiotropic platelet defect beyond gray platelet syncrome-like macrothrombocytopenia. PMID: 21989988
    47. despite extensive changes in gene expression, the chromatin state profiles and accessibility remain largely unchanged during GATA1-induced erythroid differentiation PMID: 21795386
    48. Brd3 associates with acetylated GATA1 to promote its chromatin occupancy at erythroid target genes PMID: 21536911
    49. Dll1/Notch signaling reduces GATA-1 and GATA-2 expression in bone marrow-derived mast cells. PMID: 21321116
    50. Aberrant MYB-GATA1 fusion induces leukemia in the presence of GATA1 reduced levels. PMID: 21252985

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Erythrocytes. Expressed (at protein level) in liver.
  • 数据库链接：

    KEGG: mmu:14460

    STRING: 10090.ENSMUSP00000033502

    UniGene: Mm.335973