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  4. Recombinant Mouse FAD-linked sulfhydryl oxidase ALR (Gfer)

Recombinant Mouse FAD-linked sulfhydryl oxidase ALR (Gfer)

  • 货号：
    CSB-YP009370MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009370MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009370MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009370MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009370MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Gfer; Alr; MNCb-0663FAD-linked sulfhydryl oxidase ALR; EC 1.8.3.2; Augmenter of liver regeneration
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-198
  • 氨基酸序列
    MAAPSEPAGF PRGSRFSFLP GGARSEMTDD LVTDARGRGA RHRDDTTPAA APAPQGLEHG KRPCRACVDF KSWMRTQQKR DIKFREDCPQ DREELGRHTW AFLHTLAAYY PDRPTPEQQQ DMAQFIHIFS KFYPCEECAE DIRKRIGRNQ PDTSTRVSFS QWLCRLHNEV NRKLGKPDFD CSRVDERWRD GWKDGSCD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen.
  • 基因功能参考文献：
    1. Study in mouse model of liver cirrhosis demonstrate that the lack of ALR aggravates liver fibrosis, probably by regulating mitochondrial Ca2+ homeostasis and mitochondrial dynamics in hepatic stellate cells. PMID: 30251695
    2. To test whether differences in the ratio between CHCHD4 and ALR might explain tissue-specific differences in the CHCHD4 redox state, we determined the molar ratio of both proteins in different mouse tissues. Surprisingly, ALR is superstoichiometric over CHCHD4 in most tissues. PMID: 29704824
    3. role in regulating the mitochondrial fission machinery PMID: 28646508
    4. The exogenous expression of hepatic stimulator substance (HSS was renamed augmenter of liver regeneration ALR) protected the liver from steatosis in mice with nonalcoholic steatohepatitis. PMID: 27991906
    5. this study shows that ALR can weaken ConA-induced hepatitis PMID: 27085679
    6. ALR is apparently required to ensure appropriate liver regeneration following PH in mice, and deletion of the ALR gene may delay liver regeneration in part due to impaired mitochondrial biogenesis. PMID: 25977511
    7. ALR can protect mice against acute liver injury by up-regulating the expression of regulatory T cells. PMID: 25575048
    8. From weeks 2-4 after birth, levels of steatosis and apoptosis decreased in ALR-L-KO mice, and numbers of ALR-expressing cells increased, along with ATP level PMID: 25448926
    9. As a mechanism, we suggest a direct protective effect of ALR on apoptotic and necrotic death of hepatocytes and an attenuation of inflammatory cell influx into the postischemic tissue. PMID: 24948544
    10. ALR may serve as a potential diagnostic marker of hepatocellular stress and/or acute inflammatory conditions. PMID: 23073658
    11. Growth factor erv1-like (Gfer) inhibits the COP9 signalosome subunit jun activation-domain binding protein 1 (Jab1)-mediated degradation of the cyclin-dependent kinase inhibitor p27(kip1) to restrict proliferation of hematopoietic stem cells. PMID: 21346186
    12. Gfer plays an essential pro-survival role in the maintenance of murine embryonic stem cell pluripotency by preserving the structural and functional integrity of their mitochondria, through modulation of the key mitochondrial fission factor Drp1. PMID: 20581476
    13. Growth factor erv1-like modulates Drp1 to preserve mitochondrial dynamics and function in mouse embryonic stem cells PMID: 20147447
    14. studies demonstrated a specific accumulation of full-length mouse Alrp during the early stages of spermatogenesis; the highest levels of Alrp were found in spermatogonia and primary spermatocytes PMID: 12089204
    15. review of the molecular biology of ALR [review] PMID: 16937489

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  • 亚细胞定位：
    Mitochondrion intermembrane space. Mitochondrion.
  • 组织特异性：
    Preferentially expressed in the liver and in testis.
  • 数据库链接：

    KEGG: mmu:11692

    STRING: 10090.ENSMUSP00000049186

    UniGene: Mm.28124