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  4. Recombinant Mouse Fermitin family homolog 3 (Fermt3)

Recombinant Mouse Fermitin family homolog 3 (Fermt3)

  • 货号：
    CSB-YP810365MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP810365MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP810365MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP810365MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP810365MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Fermt3
  • Uniprot No.：
  • 别名：
    Fermt3; Kind3; Urp2; Fermitin family homolog 3; Kindlin-3; Unc-112-related protein 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-665
  • 氨基酸序列
    MAGMKTASGD YIDSSWELRV FVGEEDPEAQ SVTLRVTGES HIGGVLLKIV EEINRKQDWS DHAIWWEQKR QWLLQTHWTL DKYGILADAR LFFGPQHRPV ILRLPNRRVL RLRASFSKPL FQTVAAICRL LSIRHPEELS LLRAPEKKEK KKKEKEPEEE VHDLTKVVLA GGVAPTLFRG MPAHFSDSAQ TEACYHMLSR PQPAPDPLLL QRLPRPSSLP DKTQLHSRWL DSSRCLMQQG IKAGDVLWLR FKYYSFFDLD PKTDPVRLTQ LYEQARWDLL TEEIDCTEEE MMVFAALQYH INKLTLSGDV GELASGDLGL DDLDAALNNL EVKLKGSAPS DMLDSLTTIP ELKDHLRIFR PRKLTLKGYR QYWVVFKDTT LSYYKSQDEA PGDPTQQLNL KGCEVVPDVN VSGQKFCIKL LVPSPEGMSE IYLRCQDEQQ YAQWMAACRL ASKGRTMADS SYASEVQAIL AFLSLQRAGG SNGGSGNKPQ GPEAPAEGLN PYGLVAPRFQ RKFKAKQLTP RILEAHQNVA QLSLTEAQLR FIQAWQSLPD FGISYVMVRF KGSRKDEILG IANNRLIRID LAVGDVVKTW RFSNMRQWNV NWDIRQVAIE FDEHINVAFS CVSASCRIVH EYIGGYIFLS TRERARGEEL DEDLFLQLTG GHEAF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a central role in cell adhesion in hematopoietic cells. Acts by activating the integrin beta-1-3 (ITGB1, ITGB2 and ITGB3). Required for integrin-mediated platelet adhesion and leukocyte adhesion to endothelial cells. Required for activation of integrin beta-2 (ITGB2) in polymorphonuclear granulocytes (PMNs).
  • 基因功能参考文献：
    1. expression is markedly suppressed in pathologically activated osteoclasts PMID: 29141864
    2. Structure and lipid-binding properties of the kindlin-3 pleckstrin homology domain has been reported. PMID: 27974389
    3. Mice expressing as little as 5% of kindlin-3 were viable and protected from spontaneous bleeding and infections. In vitro platelet functions, leukocyte adhesion, extravasation, and bacterial clearance were diminished. Bleeding time increased. PMID: 26438512
    4. The accumulation of HSPCs in the circulation of leukocyte adhesion deficiency type III patients, who lack Kindlin-3, underlines the conserved functions of Kindlin-3 in man and the importance of our findings for human disease. PMID: 26282877
    5. Optimal T cell activation and B cell antibody responses in vivo require the interaction between Integrin beta2 and kindlin-3. PMID: 25987740
    6. Direct binding of kindlin-3 to integrin alphaIIbbeta3 is involved in supporting integrin alphaIIbbeta3 activation and integrin alphaIIbbeta3-dependent responses of platelets and consequently contributes significantly to arterial thrombus formation. PMID: 24969775
    7. ADAP interacts with talin and kindlin-3 to promote platelet Integrin alphaIIbbeta3 activation and stable fibrinogen binding. PMID: 24523237
    8. the requirement of Kindlin-3 for effector T cells to induce alpha4beta1 and alphaLbeta2 integrin ligand binding and stabilization of integrin-ligand bonds is critical when integrin ligand levels are low, but of less importance when integrin ligand levels are high. PMID: 24089451
    9. This is the first in vivo demonstration that Kindlin-3-stabilized integrin adhesions, although essential for lymphocyte arrest on blood vessels and interstitial motility, are not obligatory for leukocyte diapedesis. PMID: 23980064
    10. the beta-2-integrin-kindlin-3 interaction is particularly important in adhesion strengthening under shear flow, and for T-cell homing to lymph nodes, but dispensable for T cell activation which occurs in a shear-free environment PMID: 23823319
    11. Kindlin-3 is central to conversion of mechanical force to intracellular signaling via calcium influx. PMID: 23144497
    12. Data indicate that Kindlin-3 specifically recognizes the integrin membrane-distal tail NPXY motif in both the beta(1A) and beta(1D) isoforms. PMID: 22989875
    13. Both talin-1 and kindlin-3 are required for induction of the high-affinity conformation of LFA-1 with an open headpiece, which results in neutrophil arrest. PMID: 22431571
    14. We investigated the role of talin-1, kindlin-3, and alpha-actinin-1 in the upregulation of alpha(4)beta(1) integrin affinity and consequent inflammatory leukocyte adhesive events PMID: 21911599
    15. Osteoclasts require their entire integrin repertoire to be regulated by kindlin-3 to orchestrate bone homeostasis. PMID: 21357746
    16. The genomic organization, gene expression and subcellular localization of murine Kindlins-1 to -3, is described. PMID: 16876785
    17. Kindlin-3 was identified as a novel and essential element for platelet integrin activation in hemostasis and thrombosis. PMID: 18278053
    18. Stable isotope labeling by amino acids in cell culture confirmed their absence and disclosed a structural defect of the red blood cell membrane skeleton in Kindlin-3-deficient erythrocytes. PMID: 18662549
    19. identify a genetic disease that severely compromises the health of the affected individuals and establish an essential role of KINDLIN-3 in integrin activation in humans PMID: 19234460
    20. Kindlin-1 and -2 directly bind the C-terminal region of beta integrin cytoplasmic tails and exert integrin-specific activation effects PMID: 19240021

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  • 亚细胞定位：
    Cell projection, podosome. Note=Present in the F-actin surrounding ring structure of podosomes, which are specialized adhesion structures of hematopoietic cells.
  • 蛋白家族：
    Kindlin family
  • 组织特异性：
    Specifically expressed in hematopoietic cells.
  • 数据库链接：

    KEGG: mmu:108101

    STRING: 10090.ENSMUSP00000037858

    UniGene: Mm.157591