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  4. Recombinant Mouse Forkhead box protein P3 (Foxp3)

Recombinant Mouse Forkhead box protein P3 (Foxp3)

  • 中文名称：
    小鼠Foxp3重组蛋白
  • 货号：
    CSB-YP857473MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Foxp3重组蛋白
  • 货号：
    CSB-EP857473MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Foxp3重组蛋白
  • 货号：
    CSB-BP857473MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Foxp3重组蛋白
  • 货号：
    CSB-MP857473MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Foxp3; Forkhead box protein P3; Scurfin) [Cleaved into: Forkhead box protein P3; C-terminally processed; Forkhead box protein P3 41 kDa form]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    1-417
  • 氨基酸序列
    MPNPRPAKPMAPSLALGPSPGVLPSWKTAPKGSELLGTRGSGGPFQGRDLRSGAHTSSSLNPLPPSQLQLPTVPLVMVAPSGARLGPSPHLQALLQDRPHFMHQLSTVDAHAQTPVLQVRPLDNPAMISLPPPSAATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPRSGTPRKDSNLLAAPQGSYPLLANGVCKWPGCEKVFEEPEEFLKHCQADHLLDEKGKAQCLLQREVVQSLEQQLELEKEKLGAMQAHLAGKMALAKAPSVASMDKSSCCIVATSTQGSVLPAWSAPREAPDGGLFAVRRHLWGSHGNSSFPEFFHNMDYFKYHNMRPPFTYATLIRWAILEAPERQRTLNEIYHWFTRMFAYFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDEFEFRKKR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2. Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7. Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1. Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development. Inhibits the transcriptional activator activity of RORA. Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4.
  • 基因功能参考文献：
    1. Epigenetic modification is also thought to take essential part into the upregulation of Foxp3 from naive CD4 + Tcells. PMID: 30003817
    2. Foxp3 vaccine promotes an immune response against tumor. PMID: 29124314
    3. Unexpectedly, although dispensable for FOXP3 expression and for the homeostasis and suppressive function of thymus-derived Treg cells, CREB negatively regulates the survival of TGF-beta-induced Treg cells, and deletion of CREB resulted in increased FOXP3+ Treg cells in the intestine and protection in a colitis model PMID: 29050947
    4. This study reports on systematic alanine-scan mutagenesis of FoxP3, assessing mutational impacts on DNA binding and transcriptional activation or repression. PMID: 29269391
    5. Foxp3 is essential for beneficial outcome of the microglial response and depends upon signalling by the immunoglobulin CD200 through its receptor (CD200R) PMID: 27731341
    6. Tbet is a critical modulator of FoxP3 expression in autoimmune graft-versus-host disease PMID: 28473623
    7. Generation of RORgammat(+) antigen-specific T17 regulatory cells from Foxp3(+) precursors in autoimmunity has been described. PMID: 28978473
    8. KLRG1 expression identifies short-lived Foxp3(+) Treg effector cells with functional plasticity in islets of NOD mice. PMID: 28850267
    9. Novel regulatory T-cells that are induced by B cells and do not express Foxp3 and IL-10 alleviate intestinal inflammation in vivo. PMID: 27581189
    10. the findings suggested that excreted-secreted antigens restricted Foxp3 expression by inhibiting TGFssRII/Smad2/Smad3/Smad4 signalling, ultimately resulting in abortion. PMID: 28300338
    11. Data (including data from studies using transgenic mice) suggest that a single oral dose of vitamin A (1) amplifies tolerogenic activity of dendritic cells migrating to lymphoid tissue and (2) up-regulates expression of Foxp3 and Cd44 in co-cultures of dendritic cells and CD4+ lymphocytes. (Foxp3 = forkhead box P3; Cd44 = homing receptor) PMID: 28917953
    12. Flicr, a long noncoding RNA, modulates Foxp3 expression and autoimmunity. PMID: 28396406
    13. Dendritic cells have the capacity to express Foxp3, which can be upregulated by exposure to Staphylococcal enterotoxin B. PMID: 27861999
    14. the Treg transcription factor Foxp3 reprograms T cell metabolism by suppressing Myc and glycolysis, enhancing oxidative phosphorylation, and increasing nicotinamide adenine dinucleotide oxidation. PMID: 28416194
    15. Data suggest that genetic or environmental factors that even moderately affect the expression of both PD-1 and FoxP3 can cause life-threatening autoimmune diseases by disrupting the T-cell homeostasis. PMID: 27410049
    16. Control of Regulatory T Cell Development by the Transcription Factor Foxp3. PMID: 28115586
    17. Foxp3 Programs the Development and Function of CD4+CD25+ Regulatory T Cells. PMID: 28115587
    18. An Essential Role for Scurfin in CD4+CD25+ T Regulatory Cells. PMID: 28115588
    19. Mechanistic analysis indicated that E47 activated expression of the transcription factor Spi-B and the suppressor of cytokine signaling 3 (SOCS3), which both downregulated Foxp3 expression. These findings demonstrate that the balance of Id3 and E47 controls the maintenance of Foxp3 expression in Treg cells and, thus, contributes to Treg cell plasticity. PMID: 27974197
    20. Foxp3 expression in lung epithelial cells, and not in Treg cells, inhibited OVA- and cockroach extract-induced asthma. PMID: 27633092
    21. Regulatory T cells have a high apoptosis rate ex vivo correlating with low c-FLIP expression. PMID: 28052242
    22. Forkhead box P3 protein (FoxP3) acts in multimodal fashion to directly activate or repress transcription, in a context- and partner-dependent manner, to govern Treg cell phenotypes. PMID: 28892470
    23. Membrane-bound DKK-1 is a novel Foxp3 postive Treg cell derived mediator to maintain immunological tolerance in T-cell-mediated autoimmune colitis. PMID: 28556921
    24. A384T mutation associated with autoimmune IPEX syndrome, induces a distinctive tissue-restricted inflammation by specifically impairing the ability of Treg cells to compete with pathogenic T cells PMID: 28778586
    25. Systemic NRF2 activation by Keap1 knockdown alleviates multiple-organ inflammation and improves the survival rate of Sf mice. PMID: 28507037
    26. CTLA-4 expressed by FOXP3(+) regulatory T cells prevents inflammatory tissue attack and not T-cell priming in arthritis. PMID: 28497863
    27. Inhibition of IL-27p28 also enhanced the suppressive capacity of adoptively transferred CD4(+) nTregs by increasing the stability of Foxp3 expression. PMID: 27488350
    28. This study suggests that the epigenetic modification of FOXP3 gene is involved in the pathogenesis of EAE. PMID: 28320131
    29. Foxp3 expression is unstable in transforming growth factor beta (TGF-beta)-induced Tregs (iTregs), while stable in thymus-derived Tregs (tTregs). To stabilize Foxp3 expression in iTregs, we introduced dCas9-TET1CD (dCas9 fused to the catalytic domain (CD) of ten-eleven translocation dioxygenase 1 (TET1), methylcytosine dioxygenase) and dCas9-p300CD (dCas9 fused to the CD of p300, histone acetyltransferase) with guide RNA. PMID: 28503202
    30. Our findings illustrate that deregulated myelopoiesis in Sf mice is mainly caused by the inflammatory reaction resulting from the lack of Treg cells. PMID: 27130185
    31. Increased methylation of "Foxp3 promoter" in Treg cells leads to impaired Treg suppressive function in biliary atresia. PMID: 27659419
    32. this study shows that inflammatory signals and Foxp3 balance mTORC1 signaling and glucose metabolism to control the proliferation and suppressive function of Treg cells PMID: 27695003
    33. results demonstrate that Tpl2 promotes inflammation in part by constraining FoxP3 expression and Treg immunosuppressive functions. PMID: 27261457
    34. Increased numbers of CD4(+)CD45RA(-)FoxP3(low) cells may cause an imbalance between Treg and Th17 cells that is mainly localized to the colon. PMID: 27895423
    35. this study shows that pretreatment with sodium selenite prevents experimental colitis by restoring Foxp3 excretion PMID: 27533281
    36. Study highlights the importance of IL-23R expression level and the instability of Foxp3+ regulatory T cells in the development of inflammatory bowel diseases. PMID: 27498708
    37. indirubin administration effectively suppressed CD4(+) T cell infiltration in the colon of DSS-induced UC mice and promoted the generation of Foxp3-expressing regulatory T cells PMID: 27396532
    38. The data suggest that RORgt expression in Tregs contributes to an optimal suppressive capacity during gut-specific immune responses, rendering Foxp3-RORgt T cells as an important effector Treg subset in the intestinal system. PMID: 26307665
    39. The ex vivo expression of transforming growth factor (TGF)-beta and factor Foxp3 were significantly higher in splenocytes of the experimental mice than the basal expression observed in the control cells. PMID: 25850927
    40. down-regulated expression in abortion mouse model PMID: 26474535
    41. These observations reveal an unexpected differential role of DJ-1 in the development of nTregs and iTregs. PMID: 26634899
    42. An IL-27/Lag3 axis enhances Foxp3+ regulatory T cell-suppressive function and therapeutic efficacy. PMID: 26013006
    43. the presence of IL-2 during the in vitro generation of iTreg cells confers resistance to Th17 conversion but that IL-2 and retinoic acid (RA) cooperate to maintain Foxp3 expression following stimulation under Th17-polarizing conditions PMID: 26583087
    44. Foxp3 has a rapid turn over in Treg partly controlled at the transcriptional level by the JAK/STAT pathway PMID: 27077371
    45. NZW Tregs were less sensitive to limiting doses of trophic cytokines, IL-2 and -33, for population homeostasis and for maintenance of FoxP3 expression PMID: 26768846
    46. Data show that tubacin can upregulate forkhead box P3 protein (Foxp3) expression of CD4(+) CD25(+) Tregs and enhances their cellular immunosuppressive capability. PMID: 26927553
    47. YY1 interrupts Foxp3-dependent target gene expression by physically interacting with Foxp3 and by directly binding to the Foxp3 target genes. PMID: 26892542
    48. the stability of Foxp3 expression is markedly compromised in T reg cells from Tet2/Tet3 double-deficient mice. PMID: 26903244
    49. Il10(-/-) Treg cells showed reduced steady-state Foxp3 expression, and polyclonal stimulation caused greater loss of Foxp3 PMID: 26224007
    50. Antigen receptor-mediated depletion of FOXP3 in induced regulatory T-lymphocytes via PTPN2 and FOXO1 PMID: 26815406

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  • 相关疾病：
    Defects in Foxp3 are the cause of the scurfy phenotype (sf). It results in a lethal disorder of immunoregulation, characterized by infections, diarrhea, anemia, thrombocytopenia, hypogonadism, gastrointestinal bleeding, lymphadenopathy and leukocytosis.
  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 组织特异性：
    High level of expression in thymus and spleen.
  • 数据库链接：

    KEGG: mmu:20371

    STRING: 10090.ENSMUSP00000041953

    UniGene: Mm.182291