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  4. Recombinant Mouse G/T mismatch-specific thymine DNA glycosylase (Tdg)

Recombinant Mouse G/T mismatch-specific thymine DNA glycosylase (Tdg)

  • 货号：
    CSB-YP023342MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP023342MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP023342MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP023342MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP023342MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tdg; G/T mismatch-specific thymine DNA glycosylase; EC 3.2.2.29; C-JUN leucine zipper interactive protein JZA-3; Thymine-DNA glycosylase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-421
  • 氨基酸序列
    MDAEAARSYS LEQVQALYSF PFQQMMAEVP NMAVTTGQQV PAVAPNMATV TEQQVPEDAP VQEPAPEAPK RRKRKPRAAE PQEPVEPKKP ATSKKSGKST KSKEKQEKIT DAFKVKRKVD RFNGVSEAEL LTKTLPDILT FNLDIVIIGI NPGLMAAYKG HHYPGPGNHF WKCLFMSGLS EVQLNHMDDH TLPGKYGIGF TNMVERTTPG SKDLSSKEFR EGGRILVQKL QKYQPRIAVF NGKCIYEIFS KEVFGVKVKN LEFGLQPHKI PDTETLCYVM PSSSARCAQF PRAQDKVHYY IKLKDLRDQL KGIERNADVQ EVQYTFDLQL AQEDAKKMAV KEEKYDPGYE AAYGGAYGEN PCNGEPCGIA SNGLTAHSAE PRGEAAPSDV PNGQWMAQSF AEQIPSFNNC GTREQEEESH A
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
  • 基因功能参考文献：
    1. These findings suggest that the catalytic and scaffolding activities of TDG are essential for retinoic acid-dependent gene expression and provide important insights into the mechanisms underlying targeting of TET-TDG complexes. PMID: 28538185
    2. The DNA demethylation marks are dynamically regulated in both in vivo and in vitro aging conditions, which are associated with Tet3 over-expression and Tdg repression. PMID: 25982203
    3. TDG, as a new coactivator, promotes beta-catenin/TCFs transactivation and functionally cooperates with CBP in canonical Wnt signaling. PMID: 24748645
    4. Embryonic lethality in Tdg (-/-) embryos is due, in part, to the reduction of noradrenaline levels. PMID: 22200605
    5. data suggest that oxidation of 5-methylcytosine by Tet proteins followed by TDG-mediated base excision of 5-carboxylcytosine constitutes a pathway for active DNA demethylation PMID: 21817016
    6. Results demonstrate that TDG is required for normal mammalian development and the establishment of proper promoter/enhancer DNA methylation patterns that are conducive to transcription during embryogenesis PMID: 21722948
    7. TDG-dependent DNA repair has evolved to provide epigenetic stability in lineage committed cells PMID: 21278727
    8. Association of CBP/p300 acetylase and TDG links DNA repair and transcription. PMID: 11864601
    9. binds SUM0-1; TDGb was also shown to interact with the promyelocytic leukemia protein (PML) in vitro as well as to colocalize with this protein to nuclear bodies in transfected cells PMID: 15569683
    10. TDG sumoylation promotes intramolecular interactions with amino- and carboxy-terminal SUMO-1 binding motifs that dramatically alter the biochemical properties and subcellular localization of TDG PMID: 17060459
    11. Data report the characterization of interactions between Dnmt3b and both thymine-DNA glycosylase (Tdg) and methyl-CpG binding domain protein 4. PMID: 18452947

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Uracil-DNA glycosylase (UDG) superfamily, TDG/mug family
  • 数据库链接：

    KEGG: mmu:21665

    STRING: 10090.ENSMUSP00000121000

    UniGene: Mm.284252