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  4. Recombinant Mouse Gamma-aminobutyric acid receptor subunit alpha-1 (Gabra1), partial

Recombinant Mouse Gamma-aminobutyric acid receptor subunit alpha-1 (Gabra1), partial

  • 货号：
    CSB-YP009140MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009140MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009140MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009140MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009140MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gabra1
  • Uniprot No.：
  • 别名：
    Gabra1; Gabra-1Gamma-aminobutyric acid receptor subunit alpha-1; GABA(A) receptor subunit alpha-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel. The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer. The alpha1/beta2/gamma2 receptor and the alpha1/beta3/gamma2 receptor exhibit synaptogenic activity. GABRA1-mediated plasticity in the orbitofrontal cortex regulates context-dependent action selection. Functions also as histamine receptor and mediates cellular responses to histamine.
  • 基因功能参考文献：
    1. This experiments show that loop F of the alpha-subunit governs the potency of GABA on alpha2 and alpha5-GABAA receptors in vitro as well as in vivo. PMID: 29109059
    2. This results of this study concluded that targeted deletion of Gabra1 causes moderate adaptations of GABAergic synapses in CA1 pyramidal cells PMID: 28643105
    3. We used a low-affinity antagonist to show that GABAA-receptor saturation occurs at this synapse but does not underlie frequency-invariant transmission. PMID: 28009294
    4. Results suggest that alpha1, alpha5 and gamma2-containing hippocampal GABA type A receptors complexes play an essential role in spatial learning and memory in which targeted disruption of the alpha1 subunit produces profound deficits. PMID: 27155990
    5. This study suggests that movement of loop G in the beta1 strand of the GABAA receptor alpha1 subunit is involved in a conformational rearrangement associated with channel activation PMID: 27981574
    6. These data suggest that GABA, acting through both presynaptic GABAA and GABAB receptors, modulate the amplitude and short-term plasticity of excitatory synapses, a result not possible from activation of either receptor type alone PMID: 27061582
    7. Nbea targets glutamate and GABA receptors to the synapse via distinct molecular pathways by interacting with specific effector proteins. PMID: 25934101
    8. Heterozygous loss of alpha1 subunit disinhibits the ventrobasal nucleus by substantially reducing phasic GABAergic currents and increases the reticular nucleus inhibition by prolonging phasic currents. PMID: 25447232
    9. the identity and location of distinct GABAAR subunits within the cellular networks of the mouse dorsal raphe nucleus and that stress impacts on the expression levels of particular subunits at the gene and protein level PMID: 24973971
    10. This study demonstrated that Adolescent-onset GABAA alpha1 silencing regulates reward-related decision making in mice. PMID: 26096050
    11. alpha1 Receptor subunits are co-expressed with beta2 and delta subunits along the extrasynaptic membranes in the dentate gyrus. PMID: 24055402
    12. Enduring loss of tonic but not phasic GABAAR currents critically contributes to the prolonged amygdala disinhibition subsequent to chronic stress. PMID: 24758222
    13. these findings suggest that gephyrin may be a key factor in BDNF-dependent GABAAR regulation in the amygdala. PMID: 24096136
    14. Gabra1 receptors are expressed in the ventral thalamus in an absence epilepsy model. PMID: 24417662
    15. The effects of heterozygous knock-out of GABAAR alpha1 on the expression and physiology of GABAARs in the mouse cortex, were determined. PMID: 23744069
    16. GABA(A) receptor alpha subunits differentially contribute to diazepam tolerance after chronic treatment. PMID: 22912786
    17. Cell-type specifici deletion of Gaba(a)alpha1 in corticotropin-releasing factor-containing neurons enhances anxiety and disrupts fear extinction. PMID: 22992651
    18. haploinsufficiency can produce epilepsy PMID: 22812724
    19. Further support for an interaction between GABA(A) and metabotropic glutamate receptor subtype(mGlu)4 receptors is obtained in the mGlu4 knockout mouse which displayed reduced binding of the GABA(A)receptor in cerebellar parallel fibers. PMID: 22145864
    20. Our results also have potential implications for the therapeutic practices that involve exposure to GABA-A Receptor modulators during neurodevelopment. PMID: 21732488
    21. These data show that enhanced, rather than compromised, GABA(A) receptor-mediated inhibition occurs in cortico-thalamic networks of SSADH(-/-) mice. PMID: 21526163
    22. Since DOR over-expression increases cortical tolerance to hypoxia, an increase in GABA receptors expression (an energy-costing process) may not be necessary in the cortex with DOR over-expression PMID: 21789893
    23. GABA(A) receptor dynamics are regulated by interaction with purinergic P2X(2) receptors PMID: 21343285
    24. Arc and Fos, increased about 50% in the alpha1 mutants. Behavioral effects (loss of righting reflex, rotarod) of flurazepam and pentobarbital were not different between alpha2 mutants and wild-type, but they were enhanced for alpha1 knock-in mice. PMID: 21056629
    25. PKC-dependent phosphorylation of the alpha4 subunit plays a significant role in enhancing the cell surface stability and activity of GABA(A)R subtypes that mediate tonic inhibition PMID: 20940303
    26. Report gabra1 immunoreactivity located in the nucleus of spermatogonia, spermatocytes and round spermatids in mouse testes. PMID: 20712007
    27. This study demonstrated that amygdala expression of the alpha1-GABA(A) receptor subunit is required for normal benzodiazepines effects on sedation, locomotion, and seizure inhibition, but not for anxiolysis. PMID: 20505082
    28. Differential cell surface expression of GABAA receptor alpha1, alpha6, beta2 and beta3 subunits in cultured mouse cerebellar granule cells influence of cAMP-activated signalling. PMID: 11902122
    29. significance of alpha1 subunit expression and its involvement in the regulation of central nervous system excitability PMID: 12183661
    30. Distinct alpha subunit contributions create heterogeneity in developmental acceleration of IPSC decay in neocortex. PMID: 12433958
    31. deletion of the alpha1 subunit of GABAA receptors reduces actions of alcohol and other drugs PMID: 12490572
    32. GABA(A) receptor alpha1 subunit-containing receptors appear to influence the motor-stimulating effect of ethanol and the sedative/hypnotic effects of some anesthetics PMID: 12606632
    33. In mouse brain cerebral cortical neurons, the dileucine motif peptide increases GABA-gated chloride currents of native GABA(A) receptors. PMID: 12707262
    34. Synergistic interaction whereby activation of NMDA receptors leads to enhanced GABAA receptor-mediated activity through a Ca2+-dependent mechanism. PMID: 12730335
    35. mice lacking the GABAA receptor alpha1 subunit displayed an increased density of dendritic filopodia during the second and third postnatal weeks. PMID: 14622913
    36. only alpha1 GABAA receptor-containing circuits were found to drive visual cortical plasticity PMID: 15017002
    37. The srain differences in the BZ/GABAA receptor system occur, at least in part, as a function of parental care. Such findings may reflect a mammalian example of an indirect genetic effect mediated by maternal care. PMID: 15085086
    38. The 5-HT1AR-mediated regulation of GABA(A)R alpha subunit expression is subject to genetic modification which operates early in postnatal life to influence the expression of GABA(A)R alpha subunits at the transcriptional level. PMID: 15254090
    39. In olfactory bulb of mitral cell dendrites GABA(A) receptors are distributed in a perisynaptic domain that surrounds the presynaptic specialization. PMID: 15579148
    40. A developmental sequence of postsynaptic events is proposed in which diffuse extrasynaptic Gabra1 subunits accumulate at inhibitory synapses to form postsynaptic clusters, mostly colocalizing with glycine receptor clusters in the adult. PMID: 15610161
    41. This paper shows that the alpha1 -/- mice do not show a large reduction in GABAergic synapses but do retain long-lasting postsynaptic currents into adulthood. PMID: 15758057
    42. Data show that gamma-aminobutyric acidA (GABA(A)) receptor alpha1-/- mice exhibit postural and kinetic tremor and motor incoordination that is characteristic of essential tremor disease. PMID: 15765150
    43. Although inverse agonists acting at alpha5-containing receptors reduce ethanol self-administration, alpha5 subunits may not be essential to signaling ethanol reward. PMID: 16253225
    44. our results do not support a significant compensatory synthesis of other GABAA receptor subunits in the cerebellum of alpha1 -/- mice PMID: 16277610
    45. Derepression of GABAAR-alpha1 expression upon downregulation of c-myc represents a unique apoptotic mechanism and a distinct function for the alpha1 subunit, independent of its role as a component of the GABAAR in the plasma membrane. PMID: 16294320
    46. Whole-cell patch-clamp recordings in acute slices revealed that PCs from alpha1(0/0) mice lack spontaneous and evoked IPSCs, demonstrating that assembly of functional GABAA receptors requires the alpha1 subunit. PMID: 16554475
    47. a conserved lysine in the TM2-3 of alpha1, beta2, and gamma2 of the GABA-A receptor has an asymmetric function in different GABAA subunits PMID: 16627470
    48. Strategy for creating GABRA1 knockin mice in which a point mutant allele replaces the wild type allele in a conditional manner, e.g., in a tissue-specific and/or temporally restricted pattern. PMID: 16832820
    49. Results describe an up-regulation in the tyrosine kinase phosphorylation of GABA(A )receptor beta(2)- and gamma(2)-subunits following chronic ethanol exposure, but no effect on alpha(1)-subunit in mouse cultured cortical neurons. PMID: 16927170
    50. Results suggest that CaMK-II is capable of directly phosphorylating GABA(A) receptors and activating endogenous tyrosine kinases to phosphorylate the gamma2 subunit in NG108-15 cells. PMID: 17442679

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily, GABRA1 sub-subfamily
  • 数据库链接：

    KEGG: mmu:14394

    STRING: 10090.ENSMUSP00000020707

    UniGene: Mm.439668