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  4. Recombinant Mouse Gamma-interferon-inducible lysosomal thiol reductase (Ifi30)

Recombinant Mouse Gamma-interferon-inducible lysosomal thiol reductase (Ifi30)

  • 货号：
    CSB-YP872152MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP872152MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP872152MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP872152MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP872152MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ifi30; Gilt; Ip30; Gamma-interferon-inducible lysosomal thiol reductase; EC 1.8.-.-; Gamma-interferon-inducible protein IP-30; Lysosomal thiol reductase IP30
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    55-230
  • 氨基酸序列
    PSPPVR VSLYYESLCG ACRYFLVRDL FPTWLMVMEI MNITLVPYGN AQERNVSGTW EFTCQHGELE CRLNMVEACL LDKLEKEAAF LTIVCMEEMD DMEKKLGPCL QVYAPEVSPE SIMECATGKR GTQLMHENAQ LTDALHPPHE YVPWVLVNEK PLKDPSELLS IVCQLYQGTE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation.
  • 基因功能参考文献：
    1. GILT has a role in maintaining cysteine cathepsin proteolytic efficiency in phagosomes PMID: 25253686
    2. These results show that wild-type mouse fibroblasts are relatively resistant and GILT(-/-)mouse fibroblasts are susceptible to Dengue virus 2 entry and replication. PMID: 23642353
    3. Data indicate that in the absence of Gamma-interferon-inducible lysosomal thiol reductase (GIL, CD4-positive T cell responses to major house dust mite allergen Der p 1 are significantly reduced. PMID: 23326313
    4. GILT has a critical role in regulating CD4(+) T-cell tolerance to an endogenous skin-restricted antigen relevant to controlling autoimmunity and generating effective immunotherapy for melanoma. PMID: 21833020
    5. gene knock-outs increase oxidative stress and autophagy, which is regulated by altering glutathione synthesis and oxidation PMID: 21640818
    6. GILT has limited yet unexpected effect on self-peptide species presented by major histocompatibility complex class II antigens. PMID: 20485683
    7. study shows GILT facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T cells or cross-presentation;initiation of gB-specific CD8+ T cell response during HSV-1 infection or cross-priming is GILT-dependent PMID: 20538950
    8. the thiol reductase activity of GILT is its essential function in MHC class II-restricted Ag processing PMID: 17142755
    9. findings show that endogenously produced peptide-IgG is processed by transduced splenic B cells in the lysosomes/endosomes in a GILT-dependent manner, and this endocytic processing pathway is essential for generating tolerogenic B cells PMID: 18606668
    10. Replication of Listeria monocytogenes in GILT-negative macrophages, or macrophages expressing an enzymatically inactive GILT mutant, is impaired because of delayed escape from the phagosome PMID: 18815593
    11. Expression of GILT in T-lymphocytes increases as a function of maturity, thereby reducing sensitivity of T-cells and consequences of autoimmune responses; a role of GILT in the immune system may be in reducing the impact of T-cell autoreactivity. PMID: 19124716
    12. GILT facilitated the processing and presentation to antigen-specific T cells of protein antigens containing disulfide bonds. PMID: 11701933

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  • 亚细胞定位：
    Secreted. Lysosome.
  • 蛋白家族：
    GILT family
  • 数据库链接：

    KEGG: mmu:65972

    STRING: 10090.ENSMUSP00000034299

    UniGene: Mm.30241