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  4. Recombinant Mouse Glucokinase (Gck)

Recombinant Mouse Glucokinase (Gck)

  • 货号：
    CSB-YP009319MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009319MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009319MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009319MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009319MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Gck; Gk; Hexokinase-4; HK4; EC 2.7.1.1; Glucokinase; Hexokinase type IV; HK IV; Hexokinase-D
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-465
  • 氨基酸序列
    MLDDRARMEA TKKEKVEQIL AEFQLQEEDL KKVMSRMQKE MDRGLKLETH QEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEAGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN IVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDRQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GNSGELDEFL LEYDRMVDES SVNPGQQLYE KIIGGKYMGE LVRLVLLKLV EENLLFHGEA SEQLRTRGAF ETRFVSQVES DSGDRRQILN ILSTLGLRPS VADCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPNCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant. Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues. Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell. In pancreas, plays an important role in modulating insulin secretion. In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage. Required to provide D-glucose 6-phosphate for the synthesis of glycogen. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate.
  • 基因功能参考文献：
    1. These data also imply an etiological role of GCK in diet-induced diabetes. PMID: 29915142
    2. Long-term treatment with octanoic acid increased cellular glucose uptake in MIN6 cells by up-regulating the expression of glucokinase (GK). PMID: 29885841
    3. a nuclear import of glucokinase mediated by a redundant mechanism, involving a nuclear localization signal, and which is modulated by its SUMOylation, is reported. PMID: 28648619
    4. Glucokinase governs an alpha-cell metabolic pathway that suppresses secretion at or above normoglycemic levels; abnormal suppression of glucagon secretion deregulates hepatic glucose metabolism and, over time, induces a pre-diabetic phenotype. PMID: 29416045
    5. GCK-dependent glycolysis regulates Treg cell migration. PMID: 29166588
    6. GCK expression is regulated by nutrient-sensing O-linked beta-N-acetylglucosaminylation cycling in liver. PMID: 27520373
    7. Absence of Gck expression did not prevent the glucose responsiveness of glucose-excited or glucose-inhibited Sf1 neurons in either sex. Thus Gck in the VMN plays a sex-specific role in the glucose-dependent control of autonomic nervous activity; this is, however, unrelated to the control of the firing activity of classical glucose-responsive neurons. PMID: 27422385
    8. Using a mutant GCK gene (GCK 262) with a knocked out cytosine at position 2643 results in lower protein expression and more ubiquitination-led protein degradation compared with wild-type GCK (GCK 261) PMID: 26752353
    9. lncLGR facilitates the recruitment of hnRNPL to the GCK promoter and suppresses GCK transcription. PMID: 26904944
    10. Given that acetylated GKRP may affiliate with type-2 diabetes mellitus (T2DM), understanding the mechanism of GKRP acetylation in the liver could reveal novel targets within the GK-GKRP pathway, for treating T2DM and other metabolic pathologies. PMID: 26620281
    11. These results suggest a mechanism for integrative control over GCK activation and, therefore, glucose metabolism and insulin secretion through regulation of cytoplasmic Ca(2+) levels. PMID: 26698632
    12. GHR knockdown caused increased glucokinase mRNA and protein levels. PMID: 26015548
    13. results suggest that chronic suppression of hepatic glucokinase has a small influence on intertissue (liver-to-BAT as well as liver-to-beta-cell) metabolic communication PMID: 25817793
    14. Atf3-silencing reversed ethanol-mediated Gck down-regulation and beta-cell dysfunction, followed by the amelioration of impaired glucose tolerance and insulin resistance. PMID: 25074928
    15. Suggest heterozygous glucokinase knockout mouse as a translatable model of human type 2 diabetes. PMID: 24772483
    16. Chronic treatment with glucose kinase activators in animal models of diabetes provided sustained lowering of blood glucose. PMID: 24772484
    17. In the liver-specific glucokinase knockout mouse reduced expression of glucokinase in the liver induces diabetic cardiomyopathy. PMID: 24447392
    18. Glucokinase is regulated by the ubiquitin-proteasome system to avoid misfolding and reduced activity of the enzyme. PMID: 24028089
    19. IRS-2 signalling is important for maintaining the activity of liver glucokinase. PMID: 23560040
    20. Hepatic GK overexpression in obesity-resistant mice promoted weight gain, while hepatic GK knockdown in obesity-prone mice attenuated weight gain with increased adaptive thermogenesis. PMID: 23217261
    21. SUMOylation of pancreatic glucokinase regulates its cellular stability and activity PMID: 23297408
    22. Changes in gluconeogenesis, delayed development of GCK and impaired hepatic glycogen synthesis in the liver potentially lead to the onset and progression of MODY2. PMID: 23291412
    23. A novel Gck mutation in mice, with relevance in humans, leading to glycaemic disease. PMID: 22698525
    24. Fed LiRiKO mice displayed loss of Akt Ser473 phosphorylation and reduced glucokinase and SREBP1c activity in the liver. PMID: 22521878
    25. beta-cell glucokinase appears to be essential for liraglutide-mediated insulin secretion, but liraglutide may improve glycemic control, steatosis, and beta-cell death in a glucokinase-independent fashion PMID: 22569791
    26. Generation of N-ethyl-N-nitrosourea (ENU) diabetes models in mice demonstrates genotype-specific action of glucokinase activators. PMID: 21921030
    27. Glucagon-like peptide 1 stimulates post-translational activation of glucokinase in pancreatic beta cells PMID: 21454584
    28. The findings indicate that the absence of glucokinase inhibition by glucose 6-phosphate probably led to increased glycolysis and blocked glyceroneogenesis in the mouse model. PMID: 20623219
    29. Data show that binding and activation of glucokinase by PFK-2/FBPase-2 in beta-cells is promoted by glucose, resulting in an enhancement of insulin secretion at stimulatory glucose concentrations, without affecting basal insulin secretion. PMID: 20702580
    30. GCK inactivation and down-regulation were mediated by ethanol metabolism-generated peroxynitrite. PMID: 20855893
    31. I. sinclarii is effective in lowering blood glucose due to the upregulation of glucokinase (Gk-rs1) and downregulation of hydroxyprostaglandin dehydrogenase. PMID: 20954077
    32. results show that although HNF-6 is not required to open chromatin of the hepatic promoter of the glucokinase gene, it stimulates transcription of the glucokinase gene in the liver PMID: 12189444
    33. MRG1 and GK are cannabinoid-regulated genes and that they may be involved in the vascular and hypothalamic effects of cannabinoids, respectively. PMID: 12237329
    34. Insulin upregulates NO and causes S-nitrosylation of glucokinase(GK). Inhibition of NO synthase blocks insulin-stimulated changes in GK secretory granules and GK conformation. PMID: 12707306
    35. phosphorylation status of BAD protein helps regulate glucokinase activity PMID: 12931191
    36. Hepatic glucokinase is required for the synergistic action of ChREBP and SREBP-1c on glycolytic and lipogenic gene expression PMID: 14985368
    37. glucokinase is an integral component of the insulin secretory granule and does not translocate during glucose stimulation. PMID: 15331544
    38. Hypothesis that the glucokinase gene might be expressed in the pituitary corticotrophic cells was therefore reexamined using mRNA in situ hybridization and immunohistochemical techniques but was not proven. PMID: 16804059
    39. Overexpression of insulin receptor substrate 2 in beta cells of high diet-fed glucokinase deficient(+/-) mice partially prevented diabetes by increasing beta cell mass. PMID: 17200721
    40. Glucokinase plays a key role in the neuroendocrine regulation of metabolic economy. PMID: 17218412
    41. The hepatic abundance of glucokinase is reduced in Phosphoinositide-dependent kinase-1 deficient mice. PMID: 17267763
    42. Role of a bound glucokinase protein fraction in the regulation of insulin granule movement along tubulin filaments. PMID: 17287461
    43. PSN-GK1 is a potent glucokinase activaator and is active as an antidiabetic agent in diabetic animal models. PMID: 17415548
    44. inhibition to liver glucokinase probably is the primary pathogenesis in alloxan-induced diabetes, prompting elevation of serum glucose content that leads to a further decrease in insulin secretion PMID: 17768029
    45. adipocyte-derived Wnt signalling molecules regulate insulin secretion, glucokinase gene transcription and beta cell proliferation PMID: 17994217
    46. PFK-2/FBPase-2 protein rather than its product fructose 2,6-P(2) is the over-riding determinant of glucose-induced insulin secretion through regulation of glucokinase activity or subcellular targeting. PMID: 18039179
    47. Between these two strategies to activate glycogen deposition in the absence of GK, embryonic livers choose to express massive levels of HKI and HKII. PMID: 18165236
    48. mRNA levels of Gck, Glp1r, and Pdx1 were increased in pancreatic RNA from both CJC-1134-PC- and/or Ex-4-treated mice. PMID: 18313669
    49. HNF-4 and Foxo1 are required for reciprocal transcriptional regulation of glucokinase and glucose-6-phosphatase genes in response to fasting and feeding PMID: 18805788

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Mitochondrion.
  • 蛋白家族：
    Hexokinase family
  • 数据库链接：

    KEGG: mmu:103988

    STRING: 10090.ENSMUSP00000099984

    UniGene: Mm.220358