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  4. Recombinant Mouse Grainyhead-like protein 3 homolog (Grhl3)

Recombinant Mouse Grainyhead-like protein 3 homolog (Grhl3)

  • 中文名称：
    小鼠Grhl3重组蛋白
  • 货号：
    CSB-YP691828MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Grhl3重组蛋白
  • 货号：
    CSB-EP691828MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Grhl3重组蛋白
  • 货号：
    CSB-EP691828MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Grhl3重组蛋白
  • 货号：
    CSB-BP691828MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Grhl3重组蛋白
  • 货号：
    CSB-MP691828MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Grhl3
  • Uniprot No.：
  • 别名：
    Grhl3; Get1; Som; Tfcp2l4; Grainyhead-like protein 3 homolog; Transcription factor CP2-like 4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-603
  • 氨基酸序列
    MSNELDFRSV RLLKNDPVSF QKFPYSNEDE AWKTYLENPL TAATKAMMRV NGDEESVAAL SFLYDYYMGP KEKRILSSST GGRNDQGKKF YHSMDYEPDL APLESPTHLM KFLTENVSGS PDYTDQLKKN NLLGLEGVLP TPGKTNTVPP GPSKLEASSM DSYLLPASDI YDNGSLNSLF ESIHGVPPTQ RWQPDSTFKD DPQESLLFPD ILKTSPDPPC PEDYPGLKSD FEYTLGSPKA IHIKAGESPM AYLNKGQFYP VTLRTPAGGK GLALSSSKVK SVVMVVFDND KVPVEQLRFW RHWHSRQPTA KQRVIDVADC KENFNTVQHI EEVAYNALSF VWNVNEEAKV FIGVNCLSTD FSSQKGVKGV PLNLQIDTYD CGAGTERLVH RAVCQIKIFC DKGAERKMRD DERKQFRRKV KCPDSSNNAG IKGCLLSGFR GNETTYLRPE TDLETQPVLF IPNLHFSSLQ RPGGVVPSAG HSSSDRLPLK RTCSPFAEEF EPLPSKQAKE DDLQRVLLYV RRETEEVFDA LMLKTPDLKG LRNAISEKYG LPEENICKVY KKCKRGILVN MDNNIIQHYS NHVAFLLDMG ELDGKIQIIL KEL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor playing important roles in primary neurulation and in the differentiation of stratified epithelia of both ectodermal and endodermal origin. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes. Essential for epidermal differentiation and barrier formation at the end of embryogenesis with TGM3 as critical direct target. Exhibits functional redundancy with GRHL2 in epidermal morphogenetic events such as eyelid fusion and epidermal wound repair. Despite being dispensable during normal epidermal homeostasis in the adulthood, is again required for barrier repair after immune-mediated epidermal damage, regulates distinct gene batteries in embryonic epidermal differentiation and adult epidermal barrier reformation after injury. Plays unique and cooperative roles with GRHL2 in establishing distinct zones of primary neurulation. Essential for spinal closure, functions cooperatively with GRHL2 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure. Also required for proper development of the oral periderm. No genetic interaction with GRHL1, no functional cooperativity due to diverse target gene selectivity.
  • 基因功能参考文献：
    1. Diminished Grhl3 expression in the hindgut is the cause of spinal NTDs in the curly tail, carrying a hypomorphic Grhl3 allele. Complete loss of Grhl3 produces a more severe phenotype in which closure fails earlier in neurulation, before onset of expression in the hindgut of wild-type embryos. Abnormal surface ectoderm function, where Grhl3 mRNA is first detected, is responsible for early spinal neurulation failure. PMID: 29397878
    2. This study demonstrated that Grainyhead-like 3 (Grhl3) deficiency in brain leads to altered locomotor activity and decreased anxiety-like behaviors in aged mice. PMID: 27907249
    3. Findings suggest that transcription factor Grainyhead-like 3 (Grhl3) may be involved in the developmental pathogenesis of craniosynostosis. PMID: 27756203
    4. Based on the results, it proposes that developmental stage-specific Grhl3 plays a significant role in circumvallate papilla (CVP) morphogenesis not by just disruption of epithelial integrity but by regulating epithelial cell proliferation, apoptosis, and migration via Shh, Wnt, and apoptosis signaling during mouse embryogenesis. PMID: 27586853
    5. Upregulation of miR21 and repression of Grhl3 by leptin mediates sinusoidal endothelial injury in experimental nonalcoholic steatohepatitis. PMID: 25658689
    6. This study identifies a GRHL3-regulated epidermal barrier repair pathway that suppresses disease initiation and helps resolve existing lesions in immune-mediated epidermal hyperplasia. PMID: 25347468
    7. decreased Grhl3 expression contributes to tumor progression and upregulation of the oncomir miR-21 in squamous cell carcinoma of the skin. PMID: 22614019
    8. The developmental transcription factor Grhl3 is a potent tumor suppressor of squamous cell carcinoma in mice. PMID: 22094257
    9. Both GRHL2 and GRHL3 bind to and regulate expression of the wound repair gene Rho GEF 19, but regulation of the barrier forming gene, Transglutaminase 1 (TGase1), is unique to GRHL3. PMID: 21081122
    10. maps to Chromosome 4, 135 Mb (66 cM) from the centromere, probably not Idb3, Wnt4, Cdc42, perlecan or Grhl-3 PMID: 15678492
    11. Loss of Grhl3 alone defines a distinct lower spinal closure defect, also with defective dorso-lateral hinge points formation PMID: 20654612
    12. folate deficiency caused a similar-sized, statistically significant increase in the frequency of exencephaly among both curly tail (Grhl3 mutant) embryos and background-matched embryos that are wild type for Grhl3. PMID: 19824061
    13. formation and maintenance of the epidermal barrier in mice are dependent on a mammalian homolog of grainy head, Grainy head-like 3[Grhl3] PMID: 15831758
    14. Unique role of Grhl3 in neurulation and epidermal morphogenesis. PMID: 16831572
    15. These findings indicate that the Get-1 and LMO4 genes interact functionally to regulate epidermal terminal differentiation. PMID: 16949565
    16. This study provides evidence for a critical role of diminished Grhl3 expression in causing spinal NTDs in the curly tail mouse model. PMID: 17720888
    17. The failure of eyelid closure is due to critical functions of Get1 in promoting F-actin polymerization, filopodia formation, and the cell shape changes that are required for migration of the keratinocytes at the leading edge during eyelid closure. PMID: 18485343
    18. Study demonstrates that the LIM-only domain protein, LMO4 serves as a functional partner of GRHL3 in its established roles, and define a new cooperative role for these factors in another developmental epidermal migration event, eyelid fusion. PMID: 18619436

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Grh/CP2 family, Grainyhead subfamily
  • 数据库链接：

    KEGG: mmu:230824

    UniGene: Mm.331972