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  4. Recombinant Mouse Guanine nucleotide-binding protein G (t) subunit alpha-3 (Gnat3)

Recombinant Mouse Guanine nucleotide-binding protein G (t) subunit alpha-3 (Gnat3)

  • 中文名称：
    小鼠Gnat3重组蛋白
  • 货号：
    CSB-YP662407MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Gnat3重组蛋白
  • 货号：
    CSB-EP662407MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Gnat3重组蛋白
  • 货号：
    CSB-EP662407MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Gnat3重组蛋白
  • 货号：
    CSB-BP662407MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Gnat3重组蛋白
  • 货号：
    CSB-MP662407MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gnat3
  • Uniprot No.：
  • 别名：
    Gnat3Guanine nucleotide-binding protein G(t) subunit alpha-3; Gustducin alpha-3 chain
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-354
  • 氨基酸序列
    GSGISSESK ESARRSKELE KKLQEDAERD ARTVKLLLLG AGESGKSTIV KQMKIIHKNG YSKQECMEFK AVIYSNTLQS ILAIVKAMAT LGIDYVNPRS REDQEQLHSM ANTLEDGDMT PQLAEIIKRL WGDPGIQACF ERASEYQLND SAAYYLNDLD RLTAPGYVPN EQDVLHSRVK TTGIIETQFS FKDLNFRMFD VGGQRSERKK WIHCFEGVTC IIFCAALSAY DMVLVEDEEV NRMHESLHLF NSICNHKYFA TTSIVLFLNK KDLFQEKVAK VHLSICFPEY TGPNTFEDAG NYIKNQFLDL NLKKEDKEIY SHMTCATDTQ NVKFVFDAVT DIIIKENLKD CGLF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.
  • 基因功能参考文献：
    1. The role of alpha-gustducin in the development of obesity and adipocyte metabolism PMID: 26692363
    2. This study provides the first evidence that alpha-gustducin is involved in the octanoylation of ghrelin. PMID: 22768248
    3. Data suggest that proteins of the taste transduction cascade (alpha-gustducin, G-protein gamma13, and phospholipase Cbeta2) are expressed in testis and are in involved in spermatogenesis. PMID: 22266327
    4. The evidence suggests that alpha-gustducin may selectively participate in signal transduction in the axon terminals of rod bipolar cells in the mammalian retina. PMID: 21200350
    5. investigated whether T2R-agonists affect (i) ghrelin release via alpha-gustducin and (ii) food intake and gastric emptying via the release of ghrelin PMID: 21245306
    6. (alpha)--gustducin may be involved in the gurmarin-sensitive sweet reception pathway and may be a key molecule for links between sweet taste receptors and cell type-specific-innervation by their matched fiber class. PMID: 20028519
    7. alpha-transducin has a role in taste receptor cells and taste cell responses that is different from alpha-gustducin PMID: 12379596
    8. In mice vallate papillae, many, but not all, bitter-responsive cells expressed alpha-gustducin. In agreement with this correlation, the incidence of cells responding to bitter stimuli was reduced by 70% in mutant mice lacking alpha-gustducin PMID: 14586025
    9. Alpha-gustducin is involed in bitter, sweet & umami [MSG, monopotassium glutamate & inosine monophosphate] taste. Umami detection involving alpha-gustducin occurs in anteriorly placed taste buds. PMID: 15342734
    10. data indicate that alpha-gustducin participates in the transduction of the sweet taste in general PMID: 16740645
    11. alpha-gustducin expression at different postnatal ages revealed a significant increase in the number of immunolabeled taste buds and alpha-gustducin-positive cells in single taste buds from week 1 to 7. PMID: 16933139
    12. Axonemal-associated localization within the midpiece and principal piece of mouse spermatozoa raises the possibility that this G protein alpha-subunit may process intracellular signals controlling sperm motility. PMID: 17021831
    13. sweet taste transduction in the palate is almost entirely dependent on gustducin PMID: 17229761
    14. Gustducin was expressed in solitary epithelial cells of the mid to upper villus, which were distributed in a regional manner with most occurring within the midjejunum. PMID: 17290008
    15. This study investigated the role of these signaling proteins in the preference for fat, starch, and starch-derived polysaccharides, using Trpm5 knockout and alpha-gustducin knockout mice. PMID: 17652359
    16. Nn important role of alpha-gus is to maintain cAMP levels tonically low to ensure adequate Ca2+ signaling. PMID: 18930056
    17. Report the requirement for Galphagust in gurmarin sensitive sweet taste responses. PMID: 19211717

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    G-alpha family, G(i/o/t/z) subfamily
  • 组织特异性：
    Expressed in taste buds (sensory organs of clustered epithelial cells) of the circumvallate and fungiform papillae of the tongue as well as in palatal taste buds at protein level. Expressed in enteroendocrine cells of the gut, such as in subsets of entero
  • 数据库链接：

    KEGG: mmu:242851

    STRING: 10090.ENSMUSP00000030561

    UniGene: Mm.332230