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  4. Recombinant Mouse Guanine nucleotide-binding protein subunit alpha-13 (Gna13)

Recombinant Mouse Guanine nucleotide-binding protein subunit alpha-13 (Gna13)

  • 货号：
    CSB-YP009585MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009585MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009585MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009585MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009585MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Gna13; Gna-13Guanine nucleotide-binding protein subunit alpha-13; G alpha-13; G-protein subunit alpha-13
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-377
  • 氨基酸序列
    MADFLPSRSV LSVCFPGCVL TNGEAEQQRK SKEIDKCLSR EKTYVKRLVK ILLLGAGESG KSTFLKQMRI IHGQDFDQRA REEFRPTIYS NVIKGMRVLV DAREKLHIPW GDNKNQLHGD KLMAFDTRAP MAAQGMVETR VFLQYLPAIR ALWEDSGIQN AYDRRREFQL GESVKYFLDN LDKLGVPDYI PSQQDILLAR RPTKGIHEYD FEIKNVPFKM VDVGGQRSER KRWFECFDSV TSILFLVSSS EFDQVLMEDR QTNRLTESLN IFETIVNNRV FSNVSIILFL NKTDLLEEKV QVVSIKDYFL EFEGDPHCLR DVQKFLVECF RGKRRDQQQR PLYHHFTTAI NTENIRLVFR DVKDTILHDN LKQLMLQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). Promotes tumor cell invasion and metastasis by activating Rho/ROCK signaling pathway. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation.
  • 基因功能参考文献：
    1. results define Galpha13 as a switch regulator of myofiber reprogramming, implying that modulations of Galpha13 and its downstream effectors in skeletal muscle are a potential therapeutic approach to treating metabolic diseases. PMID: 28920922
    2. This study demonstrated an almost 100-fold higher agonist sensitivity of the Galpha13 LARG interaction compared to the Galpha13 activation in the absence of LARG. PMID: 28465324
    3. GNA13 loss is associated with GC B-cell persistence, in which impaired apoptosis and ongoing somatic hypermutation may lead to an increased risk of lymphoma development. PMID: 26989201
    4. findings identify a Galpha13-dependent pathway that exerts dual actions in suppressing growth and blocking dissemination of germinal centre B cells that is frequently disrupted in germinal centre B-cell-derived lymphoma PMID: 25274307
    5. Direct Galpha13 binding to ITGB3 cytoplasmic domain regulates integrin outside-in signaling and thrombus formation in platelets. PMID: 24162846
    6. Galpha 13 is activated in sensory neurons mediated by lysophosphatidylinositol. PMID: 23973358
    7. Galpha13 binding to VE-cadherin mediates VE-cadherin internalization and endothelial barrier disruption and inflammation. PMID: 24590762
    8. Endothelial G13 regulates VEGFR-2 promoter activity through NF-kappaB activation. PMID: 23664862
    9. Our data show that the G(12/13) family of heterotrimeric G proteins is centrally involved in pressure overload-induced cardiac remodeling and plays a central role in the transition to heart failure. PMID: 22972902
    10. new insight into the mechanism by which p115RhoGEF is activated by Galpha(13). PMID: 21507947
    11. A functional role for JLP in the gep oncogene-regulated neoplastic signaling pathway. PMID: 21472140
    12. propose here a novel cascade of PAR1-induced Galpha(13)-DVL axis in cancer and beta-catenin stabilization PMID: 20223821
    13. In mouse pancreatic acini Galpha13 links cholecystokinin stimulation to activation of RhoA and Rac1 and amylase secretion. PMID: 19940064
    14. study demonstrates not only a function of integrin alphaIIbbeta3 as a noncanonical Galpha13-coupled receptor but also a new concept of Galpha13-dependent dynamic regulation of RhoA PMID: 20075254
    15. G12/G13 activates Gi-mediated signaling pathways which are sufficient to induce integrin alpha(IIb)beta3 activation PMID: 12183468
    16. Galpha13 is activated by endothelin receptor type B, which binds it via three intracellular loops on EDNRB PMID: 12441350
    17. platelets lacking G(q) and G(13) are completely unresponsive to diffusible stimuli such as ADP, thromboxane A(2), or thrombin. PMID: 15326177
    18. Galpha13 stimulates cell migration through Hax-1 PMID: 15339924
    19. Galpha13-mediated endodermal differentiation in P19 cells requires Ku80 PMID: 15351736
    20. U46619 was unable to induce adhesion of Gq-/- or G13-/- platelets, demonstrating that both signaling pathways are essential for this process to occur. PMID: 15505105
    21. role for the rgRGS domain in the stimulation of the GEF activity of p115RhoGEF by Galpha13 PMID: 15665872
    22. results suggest a critical role for Galpha13 in endothelial cells during vascular development, place Galpha13 as a candidate mediator of PAR1 signaling in this process, and reveal roles for Galpha13 in other cell types in the mammalian embryo PMID: 15919816
    23. the radixin-CaMKII signaling pathway is involved in Galpha(13)-mediated gene transcription, suggesting that radixin could be involved in novel signaling pathway regulated by G(13) protein PMID: 16186118
    24. The study demonstrates a link between Galpha13 signaling that regulates differentiation of F9 cells through primitive to parietal endoderm and a moesin requirement for cell survival. PMID: 16860319
    25. Results suggest a mechanism by which proto-Dbl and its effector pathways are regulated by Galpha(13)-mediated signals. PMID: 17721084
    26. Galpha(13) is haploinsufficient for adult angiogenesis in both the female reproductive system and tumor angiogenesis. PMID: 19654325

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  • 亚细胞定位：
    Membrane; Lipid-anchor. Melanosome. Cytoplasm. Nucleus. Note=Cytoplasmic in adult somatic cells, but mainly nuclear in spermatids in the testes. Translocates from the cytoplasm to the nucleus during spermatogenesis, hence predominantly observed in the cytoplasm of round spermatids but localized in the nuclei of elongating or elongated spermatids and testicular spermatozoa.
  • 蛋白家族：
    G-alpha family, G(12) subfamily
  • 组织特异性：
    Expressed in brain and testis, as well as in kidney and sperm (at protein level).
  • 数据库链接：

    KEGG: mmu:14674

    STRING: 10090.ENSMUSP00000020930

    UniGene: Mm.193925