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  4. Recombinant Mouse Heparanase (Hpse), partial

Recombinant Mouse Heparanase (Hpse), partial

  • 货号：
    CSB-YP010716MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010716MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010716MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010716MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010716MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Hpse
  • Uniprot No.：
  • 别名：
    Hpse; Hpa; Heparanase; EC 3.2.1.166; Endo-glucoronidase) [Cleaved into: Heparanase 8 kDa subunit; Heparanase 50 kDa subunit]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 表达区域：
    150-535aa
  • 氨基酸序列
    KEFKNSTYSRSSVDMLYSFAKCSGLDLIFGLNALLRTPDLRWNSSNAQLLLDYCSSKGYN ISWELGNEPNSFWKKAHILIDGLQLGEDFVELHKLLQRSAFQNAKLYGPDIGQPRGKTVK LLRSFLKAGGEVIDSLTWHHYYLNGRIATKEDFLSSDVLDTFILSVQKILKVTKEITPGK KVWLGETSSAYGGGAPLLSNTFAAGFMWLDKLGLSAQMGIEVVMRQVFFGAGNYHLVDEN FEPLPDYWLSLLFKKLVGPRVLLSRVKGPDRSKLRVYLHCTNVYHPRYQEGDLTLYVLNL HNVTKHLKVPPPLFRKPVDTYLLKPSGPDGLLSKSVQLNGQILKMVDEQTLPALTEKPLP AGSALSLPAFSYGFFVIRNAKIAACI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.
  • 基因功能参考文献：
    1. elevated expression of heparanase augmented both the innate and adaptive immune system and propagated inflammatory reactions in the murine rheumatoid arthritis model. PMID: 28401953
    2. These results show for the first time that HPSE regulates macrophage polarization as well as renal damage and repair after I/R. HPSE inhibitors could therefore provide a new pharmacologic approach to minimize acute kidney injury and to prevent the chronic profibrotic damages induced by I/R. PMID: 28970256
    3. Study reveals that HPSE stimulates the proliferation of ES cells as well as the expansion of emerging progenitors during neural differentiation. PMID: 27890389
    4. heparanase as a key mediator of macrophage activation and function in tumorigenesis and cross-talk with the tumor microenvironment. PMID: 27849593
    5. Results demonstrate that acute ischemic injury up-regulates renal heparanase expression and suggest that heparanase plays a deleterious role in the development of renal injury and kidney dysfunction. PMID: 28388547
    6. Heparanase has a role in upregulating platelet adhesion activity and thrombogenicity PMID: 27129145
    7. eNOS prevents heparanase induction and the development of proteinuria PMID: 27505185
    8. Data show that heparanase-1 (HPA-1) induced shedding of heparan sulfate chain from syndecan-1 (SDC-1) facilitated the release of vascular endothelial growth factor C (VEGF-C) from SDC-1/VEGF-C complex into the medium of hepatocarcinoma cell. PMID: 28209511
    9. the activation of intestinal heparanase contributes to intestinal injury during early sepsis by facilitating the destruction of mucosal epithelial glycocalyx and promoting inflammatory responses. PMID: 28170292
    10. Heparanase increases the inflammation in AGEs-stimulated macrophages through activating the RAGE-NF-kappaB pathway. Heparanase driven inflammation from AGEs-stimulated macrophages increases the adherence of glomerular endothelial cells (GEnCs) and augments the permeability of GEnCs. PMID: 28081450
    11. heparanase contributes to allergen-induced eosinophil recruitment to the lung. PMID: 26039697
    12. Unfractionated heparin inhibits heparanase, and reverses the activation of NF-kappaB and MAPK P38 signaling pathways to attenuate inflammatory responses induced by sepsis. These results suggest that UFH PMID: 26191183
    13. Results show a critical role for heparanase in regulating the branching and invasive behavior of normal mammary epithelia which could be the result of its mutually reciprocal feedback with MMP-14. PMID: 25735873
    14. Data suggest a potential mechanism of diabetic enteropathy, which is depending remarkably on syndecan-1 (Sdc1) and -beta-D-glucuronidase heparanase (HPSE). PMID: 25702768
    15. upregulated through NF-kB and translocated to the cell surface upon herpes simplex virus-1 infection for the removal of heparan sulfate to facilitate viral release PMID: 25912399
    16. Findings identify a dual function for HPSE in malignant melanoma with a protumorigenic extracellular activity and a tumor-suppressive nuclear action. PMID: 25745999
    17. Studies in heparanase-deficient mice established its contributions to autophagy. PMID: 26249176
    18. Heparanase is suggested to be a prognostic and diagnostic marker for oral premalignant lesions which could have a major impact on future prognosis and diagnosis of SCC of the oral cavity. PMID: 25189888
    19. Novel function of heparanase guides cancer-promoting action of TAM and heparanase expression status may be predictive of treatment potential, targeting TAM/IL-6/STAT3. PMID: 25326645
    20. Under diabetic conditions, latent heparanase, overexpressed by glomerular cells and posttranslationally activated by cathepsin L of tubular origin creates chronic inflammatory conditions and fosters macrophage-mediated renal injury. PMID: 25008182
    21. We found that heparanase induction is associated with decreased levels of CXCL10, suggesting that this chemokine exerts tumor-suppressor properties in myeloma. PMID: 24699306
    22. Because HS with unsubstituted glucosamine residues accumulates in heparanase-expressing breast cancer cells, we assumed that these HS structures are resistant to heparanase and can therefore be utilized as a heparanase inhibitor. PMID: 24753252
    23. Our findings indicated cell-autonomous HPSE1 modulates clonogenicity, proliferative potential and migration of BM-MSCs and suggested the HS-GAGs may contribute to the niche microenvironment of BM-MSCs PMID: 24624965
    24. Involvement of heparanase in the pathogenesis of psoriasis and a role for the enzyme in facilitating abnormal interactions between immune and epithelial cell subsets of the affected skin. PMID: 24169805
    25. Findings indicate an important role for heparanase in immunity and identify the enzyme as a potential target for regulation of an immune response. PMID: 24532362
    26. Heparanase has roles in lymphangiogenesis and tumor invasion in pancreatic neuroendocrine tumors PMID: 23644656
    27. Cleavage of macromolecular heparin by heparanase accelerates the release of heparin and chymase from extracellular matrices. PMID: 24344642
    28. AGE-induced macrophage migration is dependent on HPA involving RAGE-HPA-PI3K/AKT pathway. Nonenzymatic activity of HPA may play a key role in AGE-induced macrophage migration associated with inflammation in diabetic vascular complication. PMID: 23442498
    29. heparanase is important for mouse palate formation because it mediates degradation of the ECM of palatal shelves PMID: 23509775
    30. Characterization of heparanase-induced phosphatidylinositol 3-kinase-AKT activation and its integrin dependence. PMID: 23504323
    31. Heparanase activates macrophages, resulting in TLR2/3 mediated induction of cytokine expression associated with plaque progression toward vulnerability. PMID: 23162016
    32. heparanase acts as a negative modulator of AgRP signaling at MC4R, through cleavage of heparan sulfate chains presumably linked to syndecan-3 PMID: 22479599
    33. Together with CCR7 and its ligands, and probably MMP-14, heparanase controls DC trafficking. PMID: 22590508
    34. Cerebral ischemia markedly increased heparanase levels in endothelial cells and then in astrocytes. PMID: 22169133
    35. autoimmune destruction of islets in NOD mice was associated with production of catalytically active heparanase PMID: 22182841
    36. Crucial role of heparanase in the pathogenesis of diabetic nephropathy(DN) and its potential as a highly relevant target for therapeutic interventions in patients with DN. PMID: 22106160
    37. Downregulating heparanase-1 with siRNA inhibits the invasiveness of mouse hepatoma cells. PMID: 20800024
    38. These findings reveal a novel function of heparanase in maintaining mast cell homeostasis PMID: 21575986
    39. Heparanase-mediated loss of nuclear syndecan-1 enhances histone acetyltransferase (HAT) activity to promote expression of genes that drive an aggressive tumor phenotype PMID: 21757697
    40. SST0001, a chemically modified heparin, inhibits myeloma growth and angiogenesis via disruption of the heparanase/syndecan-1 axis PMID: 21257720
    41. heparanase generates a vicious cycle that powers colitis and the associated tumorigenesis: heparanase, acting synergistically with the intestinal flora, stimulates macrophage activation PMID: 21490396
    42. Chronic skin barrier disruption activates heparanase and induces gene expression changes, leading to increased growth factor interaction between epidermis and dermis, and facilitating various cutaneous changes, including wrinkle formation. PMID: 20100191
    43. heparanase induces upregulation of Th2 cytokines, resulting in inhibition of the inflammatory lesion of experimental autoimmune encephalitis PMID: 20399501
    44. A full-length heparanase gene was cloned from a mouse embryo cDNA library and determined to encode a protein of 535 amino acids. The full-length mouse gene was stably expressed in NS0 myeloma cells. PMID: 12460766
    45. expression of heparanase mRNA and VEGF, and vessel formation in mouse fractured bone PMID: 14618334
    46. HPSE-1 likely plays important roles in regulating the in vivo growth and progression of melanoma PMID: 15645118
    47. heparanase is actively involved in embryo implantation. PMID: 15749484
    48. heparanase may have a role in lymph node metastatic dissemination PMID: 15799822
    49. By monitoring in vivo activation of luciferase gene driven by the heparanase promoter, we demonstrate activation of heparanase transcription at an early stage of DTH. PMID: 16384929
    50. HPSE is involved in the pathogenesis of proteinuria in overt diabetic nephropathy by degradation of heparan sulfates PMID: 17051139

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  • 亚细胞定位：
    Lysosome membrane; Peripheral membrane protein. Secreted. Nucleus.
  • 蛋白家族：
    Glycosyl hydrolase 79 family
  • 组织特异性：
    Expressed in skin, mainly in the stratum granulosum and the first layer of the stratum corneum in the upper part of the epidermis. Also detected in hair follicles and in sebaceous glands.
  • 数据库链接：

    KEGG: mmu:15442

    STRING: 10090.ENSMUSP00000044072

    UniGene: Mm.265786