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  4. Recombinant Mouse Hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs)

Recombinant Mouse Hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs)

  • 货号：
    CSB-YP010329MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010329MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010329MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010329MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010329MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Hgs; Hrs; Hepatocyte growth factor-regulated tyrosine kinase substrate
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-775
  • 氨基酸序列
    MGRGSGTFER LLDKATSQLL LETDWESILQ ICDLIRQGDT QAKYAVNSIK KKVNDKNPHV ALYALEVMES VVKNCGQTVH DEVANKQTME ELKELLKRQV EVNVRNKILY LIQAWAHAFR NEPKYKVVQD TYQIMKVEGH VFPEFKESDA MFAAERAPDW VDAEECHRCR VQFGVVTRKH HCRACGQIFC GKCSSKYSTI PKFGIEKEVR VCEPCYEQLN KKAEGKASST TELPPEYLTS PLSQQSQLPP KRDETALQEE EELQLALALS QSEAEEKERM RQKTTYTAHP KAEPTPLASS APPAGSLYSS PVNSSAPLAE DIDPELARYL NRNYWEKKQE EARKSPTPSA PVPLTEPAAQ PGEGHTAPNS MAEAPLPETD SQPITPCSGP FSEYQNGESE ESHEQFLKAL QNAVSTFVNR MKSNHMRGRS ITNDSAVLSL FQSINTMHPQ LLELLNQLDE RRLYYEGLQD KLAQIRDARG ALSALREEHR EKLRRAAEEA ERQRQIQLAQ KLEIMRQKKQ EYLEVQRQLA IQRLQEQEKE RQMRLEQQKQ TVQMRAQMPA FPLPYAQLQA MPTAGGVLYQ PSGPTSFPAT FSPAGSVEGS PMHGVYMSQP APATGPYPSM PGTTADPSMV SAYMYPTGAP GAQAAPQAQA GPTTSPAYSS YQPTPTPGYQ SVASQAPQSL PAISQPPQTS NIGYMGSQPM SMGYQPYNMQ NLMTALPGQD ASLPAQQPYI PGQQPLYQQM APSTGPPQQQ PPVAQPAPTQ GPPAQGSEAQ LISFD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
  • 基因功能参考文献：
    1. Findings find that Hrs is indispensable for the autophagic clearance of neurodegeneration-related proteins and for the survival of hippocampal neurons in mammals. In particular, the work provides the novel insight that the loss of Hrs results in insufficient autophagic clearance and enhanced ER stress, thereby triggering JNK activation and subsequent apoptotic and necroptotic neuronal cell death. PMID: 27112194
    2. HGS expression in the nervous system is developmentally regulated.HGS is required for motor neuron function. PMID: 26115514
    3. Smooth Muscle Hgs Deficiency Leads to Impaired Esophageal Motility. PMID: 26078721
    4. ESCRT-0 protein hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is targeted to endosomes independently of signal-transducing adaptor molecule (STAM) and the complex formation with STAM promotes its endosomal dissociation. PMID: 25296754
    5. A new mechanism to modify BMP signaling by Hgs during early mouse development. PMID: 21953618
    6. Hrs is a master molecule that controls in part the degradation of STAM1 and the accumulation of ubiquitinated proteins PMID: 15640163
    7. Nedd4-2 induces binding of ENaC to Hrs, which mediates the sorting decision between ENaC degradation and recycling. PMID: 20675381
    8. Peptidergic versus nonpeptidergic specification depends on a balance between HGF-Met signaling and Runx1 extinction/maintenance in primary nociceptive neurons. PMID: 20844136
    9. These data indicated that Hrs is involved in the regulation of antigen-presentation activity through the exosome secretion. PMID: 20673754
    10. Src phosphorylates Hrs in vitro & in vivo. Hrs is phosphorylated in Src-, Yes- & Fyn-negative cells. 10-20% is phosphorylated after EGF stimulation at multiple tyrosines in different parts of Hrs by several kinases downstream of the EGF receptor. PMID: 12180964
    11. Hrs, Eps15, and STAM proteins function in a multivalent complex that sorts ubiquitinated proteins into the multivesicular body pathway. PMID: 12551915
    12. the TSG101 interaction with HRS is a crucial step in endocytic down-regulation of mitogenic signaling and this interaction may have a role in linking the functions of early and late endosomes PMID: 12802020
    13. Our results suggest that GEF-1 may induce MDCK and C3H10T1/2 cells to trans-differentiate into myoblast-like cells. PMID: 15158678
    14. the Hrs hexamer interacts with the membrane and acts as a "master molecule" that presents multiple sites for protein binding PMID: 16615908
    15. In structure-function studies of Cbl and EGFR mutants, the level of Hrs phosphorylation and rapidity of apparent Hrs dephosphorylation correlated directly with EGFR degradation. PMID: 17101784
    16. The restoration of Hrs expression increased cell proliferation and anchorage-independent growth in a mouse embryonic fibroblast line established from a Hrs knockout mouse. PMID: 17545595
    17. These data suggest that Hrs plays an important role in neural cell survival in vivo and provide an animal model for neurodegenerative diseases that are known to be commonly affected by the generation of proteinaceous aggregates. PMID: 19008375

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  • 亚细胞定位：
    Cytoplasm. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Endosome, multivesicular body membrane; Peripheral membrane protein.
  • 组织特异性：
    Ubiquitous expression in adult and fetal tissues with higher expression in testis.
  • 数据库链接：

    KEGG: mmu:15239

    STRING: 10090.ENSMUSP00000026900

    UniGene: Mm.7919