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  4. Recombinant Mouse Hepatocyte nuclear factor 3-alpha (Foxa1)

Recombinant Mouse Hepatocyte nuclear factor 3-alpha (Foxa1)

  • 货号：
    CSB-YP008794MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP008794MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP008794MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP008794MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP008794MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Foxa1; Hnf3a; Tcf-3a; Tcf3aHepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; Forkhead box protein A1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-468
  • 氨基酸序列
    MLGTVKMEGH ESNDWNSYYA DTQEAYSSVP VSNMNSGLGS MNSMNTYMTM NTMTTSGNMT PASFNMSYAN TGLGAGLSPG AVAGMPGASA GAMNSMTAAG VTAMGTALSP GGMGSMGAQP ATSMNGLGPY AAAMNPCMSP MAYAPSNLGR SRAGGGGDAK TFKRSYPHAK PPYSYISLIT MAIQQAPSKM LTLSEIYQWI MDLFPYYRQN QQRWQNSIRH SLSFNDCFVK VARSPDKPGK GSYWTLHPDS GNMFENGCYL RRQKRFKCEK QPGAGGGSGG GGSKGGPESR KDPSGPGNPS AESPLHRGVH GKASQLEGAP APGPAASPQT LDHSGATATG GASELKSPAS SSAPPISSGP GALASVPPSH PAHGLAPHES QLHLKGDPHY SFNHPFSINN LMSSSEQQHK LDFKAYEQAL QYSPYGATLP ASLPLGSASV ATRSPIEPSA LEPAYYQGVY SRPVLNTS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3'. Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas, lungs and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles. Plays a role in prostate morphogenesis and epithelial cell differentiation. FOXA1 and FOXA2 are essential for hepatic specification. FOXA1 and FOXA2 are required for morphogenesis and cell differentiation during formation of the lung. FOXA1 and FOXA2 are involved in bile duct formation; they positively regulate the binding of glucocorticoid receptor/NR3C1 to the IL6 promoter. FOXA1 and FOXA2 regulate multiple phases of midbrain dopaminergic neuron development; they regulate expression of NEUROG2 at the beginning of mDA neurogenesis and of NR4A2 and EN1 in immature mDA neurons. Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription. Inhibits NKX2-1-mediated transcription from the SFTPC promoter in lung epithel independently from DNA-binding. Involved in regulation of apoptosis. Involved in cell cycle regulation. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; activates the GCG promoter.
  • 基因功能参考文献：
    1. we propose a model in which deletion of Foxa1 from STN neurons evokes a compromised state of the STN, evident by the reduced firing activity of STN neurons and the loss of a significant fraction of STN neurons. PMID: 27934886
    2. this model system will facilitate further in vivo functional studies of Foxa1 or other factors in mammary gland development and tumor formation and progression PMID: 26919034
    3. Loss of Interdependent Binding by the FoxO1 and FoxA1/A2 Forkhead Transcription Factors Culminates in Perturbation of Active Chromatin Marks and Binding of Transcriptional Regulators at Insulin-sensitive Genes. PMID: 26929406
    4. FoxA1, FoxA2, and LIPG control the uptake of extracellular lipids for breast cancer growth. PMID: 27045898
    5. genome-wide binding sites of the forkhead/winged helix transcription factor Foxa1, which functions redundantly with Foxa2 to regulate the differentiation of midbrain dopamine neurons, were characterized. PMID: 25804738
    6. Disruption of Shp in mice alters timing of expression of genes that regulate homocysteine metabolism and the liver responses to ethanol and homocysteine. SHP inhibits the transcriptional activation of Bhmt and cystathionine gamma-lyase by FOXA1. PMID: 25701738
    7. ChIP-exonuclease of the ER pioneer factor FoxA1 identifies protected DNA with a predictable 8 bp overhang from the Forkhead motif, which s term mesas; showed that mesas occur in multiple cellular contexts and exist as single or overlapping motifs. PMID: 24373287
    8. TIP30 is a key regulator for maintaining ER(+) and ER(-)luminal pools in the mammary luminal lineage via FoxA1. PMID: 24853420
    9. Foxa1 recruited Grg3 to the Nanog promoter -2kb upstream region and switched the promoter to an inactive chromatin status represented by typical modifications in histone H3. PMID: 24803390
    10. Results indicate Foxa1 expression is required for the maintenance of prostatic cellular differentiation. PMID: 24840332
    11. FoxA1 is a lineage-specification factor that is induced by IFN-beta and supports the differentiation and suppressive function of FoxA1(+) Treg cells PMID: 24531377
    12. Results suggest that the downregulation of miR-17 expression could lead to FoxA1 overexpression in acute lung injury (ALI). PMID: 24486549
    13. FoxA1 defines prostate-specific androgen receptor recruitment. It is constitutively bound to chromatin and guides AR to specific genomic loci upon hormone exposure. PMID: 24451200
    14. Foxa1 and Foxa2 are required for formation of the intervertebral discs. PMID: 23383217
    15. This study demonistrated that the later role for Foxa genes in regulating the maintenance of dopaminergic phenotype in mDA neurons. PMID: 23637192
    16. Foxa1 and Foxa2, while redundant during development, have evolved divergent roles in the adult liver, ensuring the maintenance of both genes during evolution. PMID: 22737085
    17. Stable expression of FoxA1 promotes pluripotent P19 embryonal carcinoma cells to be neural stem-like cells. PMID: 22783724
    18. FOXA1 is direct transcriptional inhibitor of SLUG expression in prostate cancer cells and play a key role in epithelial-mesenchymal transition PMID: 22203039
    19. Forkhead transcription factor FoxA1 regulates sweat secretion through Bestrophin 2 anion channel and Na-K-Cl cotransporter 1. PMID: 22223659
    20. Study uncovered a central role for Foxa1 and Foxa2 in controlling estrogen and androgen signaling through recruitment of ERalpha and AR to their relevant targets in the liver, thereby explaining the sexual dimorphism of liver cancer in mammals. PMID: 22265403
    21. FoxA occupancy of silent genes in differentiated tissues can endow the competence of such genes to be activated aberrantly and may contribute to human disease PMID: 21935353
    22. The FoxA1 is primarily responsible for the chromatin presetting while GR supports chromatin presetting at low hormone concentration and transcriptional induction at high hormone concentration. PMID: 22001115
    23. These studies provided further insights into the gene regulatory interactions among Foxa1, Foxa2 and Shh in ventral midbrain progenitors that contribute to midbrain patterning. PMID: 21093585
    24. A critical involvement of FoxA1 in stimulating neural differentiation of pluripotent stem cells at early stages. PMID: 19916800
    25. Data demonstrate that Foxa1 and Foxa2 play crucial roles in the development and maintenance of beta-cell-specific secretory and metabolic pathways. PMID: 20534694
    26. Foxa1 acts as a pioneer transcription factor in de novo activation of Afp, by exploiting a lack of methylation at juxtaposed transposed elements PMID: 20348100
    27. Data demonstrate that FOXA1 directly regulates HOXB13 in human prostate epithelial cells, and show that this prostate-specific regulatory mechanism is conserved in mice. PMID: 20018680
    28. Stable chromatin binding protects the FoxA DNA-binding domain from acetylation to preserve chromatin binding and remodeling by FoxA factors in the absence of extracellular cues. PMID: 19897491
    29. Identification of genes regulated by HNF-3alpha. PMID: 14521963
    30. expression of genes encoding aquaporins and ion channels is maintained or even increased in Foxa1-deficient mice; identified the first transcription factor whose mutation leads to a defect in renal water homeostasis in vivo PMID: 15252040
    31. HNF-6 inhibits Foxa1 by inhibiting the activity of the TGF-beta signaling pathway. PMID: 15562441
    32. Foxa1 plays unique as well as overlapping with Foxa2 rolres during morphogenesis and in the function of adult organs. PMID: 15567715
    33. Results suggest that Foxa1 and 2 regulate signaling and transcriptional programs required for morphogenesis and cell differentiation during formation of the lung. PMID: 15668254
    34. FoxA1 binding altered the chromatin structure of both the inactive- and the hormone-activated MMTV LTR.The potential function of the FoxA1-mediated expression of the MMTV provirus for maintenance of expression in different tissues is discussed. PMID: 15748903
    35. Foxa1 and Foxa2 (forkhead box proteins A1 and A2) are required in concert for hepatic specification in mouse PMID: 15959514
    36. Foxa1 plays a pivotal role in controlling prostate morphogenesis and cell differentiation. PMID: 15987773
    37. Given that Foxa1 and Foxa2 proteins are expressed differentially in the prostate and epididymis, these data suggest that the Foxa proteins have distinct effects on AR-regulated genes in different male reproductive accessory organs. PMID: 16467259
    38. Foxa1-/- beta-cells exhibit attenuated calcium influx in response to glucose and glyburide, suggesting an insulin secretion defect either at the level or upstream of the ATP-sensitive K+ channel. PMID: 17003337
    39. GST-FOXA1 fusion protein interferes with the formation of NKX2.1 transcriptional complexes by potentially masking the latter's homeodomain DNA binding function PMID: 17220277
    40. Foxa1 and Foxa2 regulate multiple phases of midbrain dopaminergic neuron development in a dosage-dependent manner. PMID: 17596284
    41. This work identifies Foxa1 and Foxa2 as important regulators of Muc2 expression in the intestine. PMID: 18336786
    42. Foxa1 and Foxa2 predominantly occupy a distal enhancer at -6.4 kb relative to the transcriptional start site in the Pdx1 gene PMID: 19141476
    43. FoxA1 and FoxA2 move much more slowly in nuclei than other transcription factor types PMID: 19339686
    44. the results document that the hepatic FOXA family of genes are differentially regulated by amino acid availability PMID: 19415718
    45. Foxa1/2 are required for normal bile duct development through prevention of excess cholangiocyte proliferation PMID: 19436110
    46. Foxa1 and Foxa2 function both upstream of and cooperatively with Lmx1a and Lmx1b in a feedforward loop promoting mesodiencephalic dopaminergic neuron development PMID: 19607821
    47. Foxa1 and Foxa2 control the differentiation of goblet and enteroendocrine L- and D-cells in mice. PMID: 19737569

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Restricted mainly to endoderm-derived tissues (lung, liver, stomach, and small intestine). Expressed in the prostate.
  • 数据库链接：

    KEGG: mmu:15375

    STRING: 10090.ENSMUSP00000041118

    UniGene: Mm.4578