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  4. Recombinant Mouse Histidine triad nucleotide-binding protein 1 (Hint1)

Recombinant Mouse Histidine triad nucleotide-binding protein 1 (Hint1)

  • 中文名称：
    小鼠Hint1重组蛋白
  • 货号：
    CSB-YP302540MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Hint1重组蛋白
  • 货号：
    CSB-EP302540MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Hint1重组蛋白
  • 货号：
    CSB-EP302540MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Hint1重组蛋白
  • 货号：
    CSB-BP302540MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Hint1重组蛋白
  • 货号：
    CSB-MP302540MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Hint1
  • Uniprot No.：
  • 别名：
    Hint1; Hint; Pkci; Pkci1; Prkcnh1; Histidine triad nucleotide-binding protein 1; EC 3.-.-.-; Adenosine 5'-monophosphoramidase; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1; PKCI-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-126
  • 氨基酸序列
    ADEIAKAQV AQPGGDTIFG KIIRKEIPAK IIFEDDRCLA FHDISPQAPT HFLVIPKKHI SQISVADDDD ESLLGHLMIV GKKCAADLGL KRGYRMVVNE GADGGQSVYH IHLHVLGGRQ MNWPPG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. Also exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RANGAP1 and RGS17.
  • 基因功能参考文献：
    1. The absence of Hint1 gene enhances supraspinal nociceptive sensitivity in mice, including thermal, mechanical and inflammatory hyperalgesia. PMID: 27547499
    2. It was observed that HINT1 protein binds to GPCRs and NMDAR NR1 subunits in a calcium-independent manner, whereas sigma1R binding to these proteins increases in the presence of calcium. PMID: 26461475
    3. Results suggest that Hint1 deficiency leads to an increased protein level of PKC gamma in the brain and a compromised activation response of PKC gamma upon stimulation PMID: 26133792
    4. HINT1 is involved in the behavioral abnormalities induced by social isolation and exerts distinct roles in different encephalic regions. PMID: 26300541
    5. These results further support a role for HINT1 in nicotine-mediated behaviors and suggest that alterations in the gene may have differential effects on phenotype in males and females. PMID: 22827509
    6. Activated mu-opioid receptors stimulate the production of nitric oxide (NO), and the redox zinc switch RGSZ2 converts this signal into free zinc ions that are required to recruit the redox sensor PKCgamma to HINT1 proteins. PMID: 25557043
    7. lipidomic phenotype of the Hint1(-/-) mouse PMID: 25193995
    8. these mice do not provide a disease model or a means for investigating the basis of HINT1-associated neuropathy and neuromyotonia PMID: 24918641
    9. our data support a role for the HINT1 gene in mediating behaviors associated with nicotine reward and physical nicotine withdrawal, and provide insight into the role of HINT1 in nicotine dependence-like behaviors. PMID: 23810802
    10. Thus the study demonstrates a novel function of HINT1 that involves the regulation of store-operated channel-mediated Ca(2+) entry pathway PMID: 23576580
    11. Results indicate a genetic association between HINT1 variants and ND, and indicate that nicotine-induced modulation of HINT1 level may be involved in mechanisms of excess smoking. PMID: 20514075
    12. HINT1 forms homodimers in cells and that these dimers act in the cross-regulation between NMDARs and MORs. PMID: 21153910
    13. Data show that through HINT1, the MOR facilitates the cross-talk of two NO- and zinc-regulated signal-transduction pathways, PKC/Src and Raf-1/ERK1/2, implicated in the negative control of morphine effects. PMID: 21235400
    14. This study indicated a role for Hint1 and PKCgamma in modulating anxiety-related and stress-coping behaviour in mice. PMID: 21316396
    15. Hint1 protein influences the course of hepatic ischemia/reperfusion injury, and its ablation in Kupffer cells may limit the extent of the injury. PMID: 21140474
    16. widely expressed during development; pronounced expression in neuronal ganglia, epithelia, hearts, and testes from embryonic day 15 onward; deficient mice had normal life span & were apparently healthy; hint may not be a key regulator of Cdk7 activity PMID: 12748294
    17. PKCI may normally play a tumor-suppressor role PMID: 12810953
    18. deletion of Hint1 in mice enhances both spontaneous tumor development and susceptibility to tumor induction by DMBA PMID: 16186798
    19. These results demonstrate an important role of PKCI/HINT1 in modulating the behavioral response to AMPH. PMID: 17203012
    20. HINT1 immunoreactivity is primarily located in neurons and neuronal processes PMID: 18270824
    21. These results suggest that the PKCI/HINT 1 gene could be important for the mood regulation function in the CNS. PMID: 19912621

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    HINT family
  • 数据库链接：

    KEGG: mmu:15254

    STRING: 10090.ENSMUSP00000020504

    UniGene: Mm.425