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  4. Recombinant Mouse Histone-lysine N-methyltransferase EZH1 (Ezh1)

Recombinant Mouse Histone-lysine N-methyltransferase EZH1 (Ezh1)

  • 中文名称：
    小鼠Ezh1重组蛋白
  • 货号：
    CSB-YP007912MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ezh1重组蛋白
  • 货号：
    CSB-EP007912MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ezh1重组蛋白
  • 货号：
    CSB-EP007912MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ezh1重组蛋白
  • 货号：
    CSB-BP007912MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ezh1重组蛋白
  • 货号：
    CSB-MP007912MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ezh1
  • Uniprot No.：
  • 别名：
    Ezh1; Enx2Histone-lysine N-methyltransferase EZH1; EC 2.1.1.356; ENX-2; Enhancer of zeste homolog 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-747
  • 氨基酸序列
    MDIASPPTSK CITYWKRKVK SEYMRLRQLK RLQANMGAKA LYVANFAKVQ EKTQILNEEW KKLRVQPVQP MKPVSGHPFL KKCTIESIFP GFDSQDMLMR SLNTVALVPI MYSWSPLQQN FMVEDETVLC NIPYMGDEVK EEDETFIEEL INNYDGKVHG EEEMIPGSVL ISDAVFLELV DALNQYSDEE EDGHNDPSDG KQDDSKEDLP VTRKRKRHAI EGNKKSSKKQ FPNDMIFSAI ASMFPENGVP DDMKERYREL TEMSDPNALP PQCTPNIDGP NAKSVQREQS LHSFHTLFCR RCFKYDCFLH PFHATPNVYK RKNKEIKIEP EPCGTDCFLL LEGAKEYAML HNPRSKCSGR RRRRHPVVSA SCSNASASAM AETKEGDSDR DTGNDWASSS SEANSRCQTP TKQKASPAPA QLCVVEAPSE PVEWTGAEES LFRVFHGTYF NNFCSIARLL GTKTCKQVFQ FAVKESLILK LPTDELMNPA QKKKRKHRLW AAHCRKIQLK KDNNSTQVYN YQPCDHPDRP CDSTCPCIMT QNFCEKFCQC SPDCQNRFPG CRCKTQCNTK QCPCYLAVRE CDPDLCLTCG ASEHWDCKVV SCKNCSIQRG LKKHLLLAPS DVAGWGTFIK ESVQKNEFIS EYCGELISQD EADRRGKVYD KYMSSFLFNL NNDFVVDATR KGNKIRFANH SVNPNCYAKV VMVNGDHRIG IFAKRAIQAG EELFFDYRYS QADALKYVGI ERETDVF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
  • 基因功能参考文献：
    1. The combined loss of Ezh1 and Ezh2 in chondrocytes severely impairs skeletal growth in mice. PMID: 27897169
    2. identification of EZH1 as a repressor of haematopoietic multipotency in the early mammalian embryo PMID: 29342143
    3. Suggest that EZH1 and -2 are novel targets of miR-214-3p, and miR-214-3p might be one potential miRNA for the prevention of cardiac fibrosis. PMID: 27823969
    4. Ezh1 is required for neonatal heart regeneration and development. PMID: 28512107
    5. the expression level of Ezh1 determines the restoration of H3K27 methylation in the absence of the canonical EZH2-PRC2. PMID: 28254491
    6. These results clearly demonstrated an essential role of Ezh1 in the pathogenesis of hematopoietic malignancies induced by Ezh2 insufficiency, and highlighted the differential functions of Ezh1 and Ezh2 in hematopoiesis. PMID: 26219303
    7. Loss of EZH1 is associated with increased liver injury and a blunted regenerative response. PMID: 25477280
    8. Data show that histone lysine methyltransferase Ezh1 promotes toll-like receptor (TLR)-triggered inflammatory cytokine production by suppressing the Toll-interacting protein (Tollip), contributing to full activation of the innate immunity. PMID: 25687760
    9. presence of Ezh1 helps to maintain PRC2 occupancy on its target genes in myoblasts where Jarid2 is not expressed. PMID: 24352422
    10. Deletion of Ezh1 and Ezh2 from skin results in a marked change in fate determination in epidermal progenitor cells, leading to an increase in the number of lineage-committed Merkel cells, specialized subtype of skin cells involved in mechanotransduction. PMID: 23673358
    11. Ezh1 is an important histone methyltransferase for hematopietic stem cell maintenance. PMID: 23122289
    12. Ezh1 has a role in promoting mRNA transcription. PMID: 22196887
    13. while Ezh1/2-null hair follicles (HFs) arrest morphogenesis and degenerate due to defective proliferation and increased apoptosis, epidermis hyperproliferates and survives engraftment PMID: 21317239

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, EZ subfamily
  • 组织特异性：
    Expressed at high levels in kidney, adrenal gland, testis and brain.
  • 数据库链接：

    KEGG: mmu:14055

    STRING: 10090.ENSMUSP00000102906

    UniGene: Mm.5027