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  4. Recombinant Mouse Histone-lysine N-methyltransferase SETD7 (Setd7)

Recombinant Mouse Histone-lysine N-methyltransferase SETD7 (Setd7)

  • 货号：
    CSB-YP845030MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP845030MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP845030MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP845030MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP845030MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Setd7; Kiaa1717; Set7; Set9; Histone-lysine N-methyltransferase SETD7; EC 2.1.1.-; Histone H3-K4 methyltransferase SETD7; H3-K4-HMTase SETD7; SET domain-containing protein 7; SET7/9
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-366
  • 氨基酸序列
    MDSDDEVVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK FFFFDGSTLE GYYVDDALQG QGVYTYEDGG VLQGTYVDGE LNGPAQEYDS DGRLIFKGQY KDNNRHGVCW IHYPDGGSLV GEVNEDGEMT GEKIAYVYPD QRTALYGKFI DGEMLEGKLA TLMATEEGRP HFEVTSGSSV YHFDKSTSSC ISSDALLPDP YESERVYVAD SLISSAGEGL FSKVAVGPNT VMSFYNGVRI THQEVDSRDW ALNGNTLSLD EETVIDVPEP YNHVSKYCAS LGHKANHSFT PNCVYDLFVH PRFGPIKCIR TLRAVEAEEE LTVAYGYDHS PPGKSGPEAP EWYQVELKAF QATQQK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.
  • 基因功能参考文献：
    1. Murine peritoneal fibrosis was induced by intraperitoneal injection of methylglyoxal (MGO). SET7/9 expression was elevated in MGO-injected mice. PMID: 29723250
    2. spinal SET7/9 may contribute to the maintenance of CIBP PMID: 28557056
    3. the methyltransferase Set9 potentiates TGF-beta signaling by targeting Smad7, an inhibitory downstream effector. PMID: 27292644
    4. SETD7 plays an important role in intestinal epithelial cell (IEC) biology during infection. PMID: 27598373
    5. We observe Set7-mediated modification of serum response factor (SRF) and mono-methylation of histone H4 lysine 4 (H3K4me1) regulate gene expression. We conclude the broad substrate specificity of Set7 serves to control key transcriptional networks in embryonic stem cells. PMID: 27439711
    6. SETD7 is required for Wnt-driven intestinal tumorigenesis and regeneration. SETD7-dependent methylation of YAP facilitates Wnt-induced nuclear accumulation of beta-catenin. PMID: 27046831
    7. SET7/9 regulates Nos2 expression through methylation of H3K4 in beta cells. PMID: 25995453
    8. Data indicate that the ability of transcription factor Pdx1 to regulate genes in beta cells is partially dependent upon its methylation by methyltransferase Set7/9. PMID: 25713082
    9. study found that Set7 is a modifier of the Hippo pathway. Mice that lack Set7 have a larger progenitor compartment in the intestine, coinciding with increased expression of Yes-associated protein target genes. PMID: 23850191
    10. The response to hyperglycemia in vascular endothelial cells involves Set7 mediated changes in chromatin remodeling and gene expression. PMID: 22403242
    11. Set7/9-mediated methylation of p53 does not represent a major regulatory event and does not appreciably control p53 activity in vivo. PMID: 21855805
    12. Set7/9 is dispensable for p53 function in the mouse. PMID: 21855806
    13. Define a biological function for Set7 in muscle differentiation and provide a molecular mechanism by which Set7 modulates myogenic transcription factors during muscle differentiation. PMID: 21859860
    14. Pdx1-dependent Set7/9 expression may be crucial to enhancing chromatin accessibility and transcription of beta-cell genes. PMID: 21099283
    15. Methylation of p53 by Set7 mediates p53 acetylation and activity in vivo. PMID: 18280244
    16. a novel role for SET7/9 in inflammation and diabetes. PMID: 18650421
    17. Data conclude that Set7/9 is required for normal beta-cell function, likely through the maintenance of euchromatin structure at genes necessary for glucose-stimulated insulin secretion. PMID: 18984737
    18. ASCOM-MLL3 and ASCOM-MLL4 likely function as crucial but redundant H3K4MT complexes for PPARgamma-dependent adipogenesis PMID: 19047629

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, SET7 subfamily
  • 数据库链接：

    KEGG: mmu:73251

    STRING: 10090.ENSMUSP00000043492

    UniGene: Mm.192111