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  4. Recombinant Mouse Homeobox protein ARX (Arx)

Recombinant Mouse Homeobox protein ARX (Arx)

  • 中文名称：
    Recombinant Mouse Homeobox protein ARX(Arx),Yeast
  • 货号：
    CSB-YP002167MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Homeobox protein ARX(Arx),Yeast
  • 货号：
    CSB-EP002167MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Homeobox protein ARX(Arx),Yeast
  • 货号：
    CSB-EP002167MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Homeobox protein ARX(Arx),Yeast
  • 货号：
    CSB-BP002167MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Homeobox protein ARX(Arx),Yeast
  • 货号：
    CSB-MP002167MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Arx
  • Uniprot No.：
  • 别名：
    ArxHomeobox protein ARX; Aristaless-related homeobox
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-564
  • 氨基酸序列
    MSNQYQEEGC SERPECKSKS PTLLSSYCID SILGRRSPCK MRLLGAAQSL PAPLASRADQ EKAMQGSPKS SSAPFEAELH LPPKLRRLYG PGGGRLLQGA AAAAAAAAAA AAAATATGTA GPRGEVPPPP PPAARPGERQ DSAGAVAAAA AAAAWDTLKI SQAPQVSISR SKSYRENGAP FVPPPPALDE LSGPGGVAHP EERLSAASGP GSAPAAGGGT GAEDDEEELL EDEEDEEEEE ELLEDDDEEL LEDDARALLK EPRRCSVATT GTVAAAAAAA AAAVATEGGE LSPKEELLLH PEDAEGKDGE DSVCLSAGSD SEEGLLKRKQ RRYRTTFTSY QLEELERAFQ KTHYPDVFTR EELAMRLDLT EARVQVWFQN RRAKWRKREK AGAQTHPPGL PFPGPLSATH PLSPYLDASP FPPHHPALDS AWTAAAAAAA AAFPSLPPPP GSASLPPSGA PLGLSTFLGA AVFRHPAFIS PAFGRLFSTM APLTSASTAA ALLRQPTPAV EGAVASGALA DPATAAADRR ASSIAALRLK AKEHAAQLTQ LNILPGTSTG KEVC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor required for normal brain development. May be important for maintenance of specific neuronal subtypes in the cerebral cortex and axonal guidance in the floor plate.
  • 基因功能参考文献：
    1. Study characterized the disturbances to cortical interneuron development in mice modeling the two most common ARX polyalanine expansion mutations in human. We found a consistent approximately 40-50% reduction of calbindin-positive interneurons, but not Stt+ or Cr+ interneurons, within the cortex of newborn hemizygous mice for both mutant strains compared to wildtype. PMID: 28627419
    2. This report present comprehensive postnatal data for mice modelling the two most common ARX polyalanine expansion mutation and show that the phenotypic outcomes recapitulate the common features observed in patients harbouring ARX polyalanine expansion mutations. PMID: 28602636
    3. Study provides further support that the role of Arx in interneuron development is to direct appropriate migration of ventral neuronal precursors into the dorsal cortex and that the loss of Arx results in a failure of interneurons to reach the cortex and thus a deficiency in interneurons. PMID: 27287386
    4. ARX positively regulates Wnt/ beta-catenin signaling and the C-terminal domain of ARX interacts with the armadillo repeats in beta-catenin to promote Wnt/beta-catenin signaling. In addition, we found BCL9 and P300 also interact with ARX to modulate Wnt/beta-catenin signaling. PMID: 28103279
    5. Arx and Dnmt1 that are sufficient for achieving targeted generation of beta cells from adult pancreatic alpha cells. PMID: 28215845
    6. We propose a core pathway of transcription regulators, including Hdac4, involved in chromatin condensation and transcriptional repression, and one of its targets, the transcription factor Twist1, as potential drivers of the ID and infantile spasms in patients with ARX polyalanine expansion mutations PMID: 27798109
    7. an expansion of 7 alanines in the first polyalanine tract of both human ARX and mouse Arx altered enteroendocrine differentiation; conclude ARX/Arx is required for the specification of a subset of enteroendocrine cells in both humans and mice PMID: 25171319
    8. Results support a model where ARX regulates the expansion of cortical progenitor cells through repression of Cdkn1c PMID: 23968833
    9. Our data therefore suggest that strategies aiming at inhibiting the expression of Arx, or its molecular targets/co-factors, may pave new avenues for the treatment of diabetes. PMID: 24204325
    10. through interactions with FoxA2, Arx positively regulates Shh expression in the floor plate, and Shh signaling in turn activates Nkx2.2, which suppresses Arx expression. PMID: 24968361
    11. ARX in the therian mammal ancestor already played a role in male reproduction before it was recruited to the X chromosome in eutherians. PMID: 24307230
    12. Loss of Lmo1 repression may be one of the causes accounting for the phenotypic differences identified between the Arx((GCG)7)and Arx(432-455dup24) mouse models. PMID: 24122442
    13. Mice with an expansion mutation in the first polyalanine tract of Arx exhibit impaired alpha-cell specification and maintenance. PMID: 24236044
    14. ARX was expressed in the coelomic epithelial cells. PMID: 23840809
    15. This study demonistrated that arx is regi=uired for specification of the zona incerta and reticular nucleus of the thalamus in mice. PMID: 24487799
    16. nuclear import of Arx via NLS1 contributes to its transcriptional function. PMID: 23771350
    17. Pancreatic alpha-cell specific deletion of mouse Arx leads to alpha-cell identity loss. PMID: 23785486
    18. Findings indicate that the function of aristaless-related homeobox (ARX) is one of the key modifiers for hyperplasia of islet alpha-like cells in the absence of proglucagon-derived peptides. PMID: 23671715
    19. a novel and conserved role of Arx in mammalian endocrine cell development PMID: 22387004
    20. these finding demonstrate that, downstream of Neurog3, the specification of a subset of enteroendocrine subtypes relies on both Arx and Pax4, while others depend only on Arx or Pax4. PMID: 22570716
    21. study found that although Arx(E)(expanded poly-A tract) functions normally in the dorsal brain, its function in subpallial-derived populations of neurons is compromised; contrasting functions are associated with misregulation of Arx targets through the loss of the ability of Arx(E) to interact with the Arx cofactor Tle PMID: 22108177
    22. Nkx2.2 acts by reinforcing the transcriptional networks initiated by Pax4 and Arx in early committed beta- and alpha-cell, respectively. PMID: 21880149
    23. Results identified multiple new candidate targets for Arx and should help to better understand the pathophysiological mechanisms of intellectual disability and epilepsy associated with ARX mutations. PMID: 21966449
    24. these experiments identify novel genetic interactions between Nkx2.2 and Arx within the endocrine progenitor cells that ensure the correct specification and regulation of endocrine hormone-producing cells PMID: 21856296
    25. Isl-1 represents the first known activator of Arx transcription in alpha-cells, here established to be acting through the conserved Re1 and Re2 control domains PMID: 21388963
    26. The identification of the lineage determination gene aristaless-related homeobox (Arx), as methylated and repressed in beta cells, and hypomethylated and expressed in alpha cells and Dnmt1-deficient beta cells. PMID: 21497756
    27. Ablation of the alpha-cell transcription factor, Arx, resulting in a complete loss of the glucagon-producing pancreatic alpha-cell. PMID: 20592160
    28. ARX plays a role in development of forebrain and testes in mice PMID: 12379852
    29. Arx is a marker of adult neuronal stem cells and forebrain GABAergic neurons. PMID: 15376319
    30. role in regulating the expression of putative instructive signals produced in the olfactory bulb for the proper innervation of olfactory sensory axons PMID: 15677725
    31. Arx and Pax4 act as transcriptional repressors that control the expression level of one another, thereby mediating proper endocrine fate allocation in the pancreas. PMID: 15930104
    32. Activin A has opposite effects on glucagon and arx gene expression in alpha-cells compared with beta-cells, a finding that may have relevance during pancreatic endocrine lineage specification and physiological function of the adult islets PMID: 16988001
    33. In adult mice, the misexpression of Arx, rather than its overexpression, promotes a conversion of beta cells into glucagon- or PP-producing cells in vivo. PMID: 17404619
    34. critical functions of Arx in promoting neural migration and regulating basal ganglia differentiation in mice PMID: 17460091
    35. Arx acts as a novel positive regulator of embryonic myogenesis by synergizing with Mef2C and MyoD and by establishing an activating loop with Myogenin PMID: 17932502
    36. Arx directly repressed three of the identified transcription factors: Lmo1, Ebf3 and Shox2. PMID: 18799476
    37. Although Arx is necessary for the Dlx-dependent promotion of interneuron migration, it is not required for the GABAergic cell fate commitment mediated by Dlx factors. PMID: 18923043
    38. Arx(-/y);Dlx5/6(CIG) (male) mice exhibit a variety of seizure types beginning in early-life, including seizures that behaviourally and electroencephalographically resembles infantile spasms, and show evolution through development. PMID: 19439424
    39. Using an in vitro nuclear import assay, the study shows that import of Arx via nuclear localization sequence2 can be mediated by importin beta1, importin 9, or importin 13, with binding being strongest to importin beta1. PMID: 19494118

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Paired homeobox family, Bicoid subfamily
  • 数据库链接：

    KEGG: mmu:11878

    STRING: 10090.ENSMUSP00000049039

    UniGene: Mm.275547