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  4. Recombinant Mouse Homeobox protein MIXL1 (Mixl1)

Recombinant Mouse Homeobox protein MIXL1 (Mixl1)

  • 中文名称：
    小鼠Mixl1重组蛋白
  • 货号：
    CSB-YP895286MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Mixl1重组蛋白
  • 货号：
    CSB-EP895286MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Mixl1重组蛋白
  • 货号：
    CSB-EP895286MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Mixl1重组蛋白
  • 货号：
    CSB-BP895286MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Mixl1重组蛋白
  • 货号：
    CSB-MP895286MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Mixl1
  • Uniprot No.：
  • 别名：
    Mixl1; Mix; MmlHomeobox protein MIXL1; Homeodomain protein MIX; mMix; MIX1 homeobox-like protein 1; Mix.1 homeobox-like protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-231
  • 氨基酸序列
    MAAAGSQQLQ FAEGAAFPIF PAAHPGGQLL PAMRPASGLP AAPHDSRAPA ATQCFPNRDS SPTAQTPAGL DPPGPSKGSA APSAPQRRKR TSFSSEQLQL LELVFRQTMY PDIHLRERLA ALTLLPESRI QVWFQNRRAK SRRQSGKSFQ PLSSRRGVFL HCPAPGTEAR CLKPQLPLEA DVNHVPDPSM TGGGVCTSGS QSFETYSSLS EDIGSKLDSW EEHIFSALGN F
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression.
  • 基因功能参考文献：
    1. In this study, we demonstrated that forced expression of Mix-like protein 1 (encoded by Mixl1) can be used to guide contribution of mouse embryonic stem cells to endodermal organs after blastocyst injection. PMID: 25192056
    2. Embryonic localization of Mixl1 shows its co-localized with the early hematopoietic marker, Runx1, in the allantois and visceral yolk sac blood islands. PMID: 24632399
    3. At later stages, in contrast with wild-type embryos, E7.5-8.0, Mixl1 knockout (KO) KO/KO embryos lacked morphologically recognizable midline structures such as the node and notochord. PMID: 24596343
    4. These data support the hypothesis that Mixl1 directly regulates Pdgfralpha and Flk1 gene expression. PMID: 22265737
    5. results strongly suggest that Gsc is a direct target gene of Mixl1 during embryogenesis. PMID: 19711456
    6. required for the morphogenesis of axial mesoderm, the heart and the gut during embryogenesis PMID: 12117810
    7. non-cell autonomous role in nascent mesoderm in the formation and/or patterning of adjacent definitive endoderm. PMID: 12619131
    8. Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells PMID: 15673572
    9. all mice reconstituted with Mixl1-transduced bone marrow developed fatal, transplantable acute myeloid leukemia with a mean latency period of 200 days PMID: 17060613
    10. As goosecoid is itself induced in a Foxh1-dependent manner, we propose that Foxh1 initiates positive and negative transcriptional circuits to refine cell fate decisions during gastrulation PMID: 17568773
    11. Overexpression of Mixl1 appears capable of inducing an abnormal self-renewal capacity in granulocytic precursors PMID: 18056627
    12. The studies suggest that high levels of Mixl1 preferentially allocate cells to the endoderm during ESC differentiation. PMID: 19038793

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Paired homeobox family
  • 组织特异性：
    Expressed in the primitive streak of the gastrulating embryo, and marks cells destined to form mesoderm and endoderm. Present in differentiating embryonic stem cells (at protein level).
  • 数据库链接：

    KEGG: mmu:27217

    STRING: 10090.ENSMUSP00000027778

    UniGene: Mm.103647